PURPOSE:To evaluate the effects of copaiba oil on the correction of abdominal defect treated with the use of polypropylene/ polyglecaprone mesh in rats. METHODS:A defect in the abdominal wall was created and corrected with polypropylene/polyglecaprone mesh in 36 rats. They were randomly distributed into three groups: control, copaiba by oral administration (gavage) and copaiba oil dip in the mesh. Euthanasia was performed after seven, 14 and 21 post-operative days. The healing process was analyzed regarding the meshes and macroscopic and microscopic aspects. RESULTS:All animals had abdominal adhesions, which were smaller in the copaiba (gavage) group (p<0.05). In microscopy, all animals had an acute inflammation stage and the inflammatory response was best characterized by foreign body-type granulomas around the mesh fragments, which was not found in the mesh fragments within the copaiba dip group. There was a greater area of necrosis and fibrosis in the copaiba dip group compared to the control group (p<0.05). The copaiba (gavage) group had a greater quantity of collagen fibers compared to the control group. CONCLUSION:Copaiba oil administered by gavage decreased the amount of abdominal adhesions, besides accelerating the process of collagen fibers formation, without damages within the early stages of healing. However, when used by dip directly on the mesh, it had corrosive effects compromising the healing process of the abdominal wall.

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Background: The use of meshes in hernia surgical repair promoted revolution in the surgical area; however, some difficulties had come, such as a large area of fibrosis, greater postoperative pain and risk of infection. The search for new substances that minimize these effects should be encouraged. Medicinal plants stand out due possible active ingredients that can act on these problems. Aim: To check the copaiba oil influence in the repair of abdominal defects in rats corrected with Vicryl(c) mesh. Method: Twenty-four Wistar rats were submitted to an abdominal defect and corrected with Vicryl(c) mesh. They were distributed into two groups: control and copaíba via gavage, administered for seven days after surgery. The analysis of the animals took place on 8, 15 and 22 postoperative days. It analyzed the amount of adhesions and microscopic analysis of the mesh. Results: There was no statistical difference regarding the amount of adhesions. All animals had signs of acute inflammation. In the control group, there were fewer macrophages in animals of the 8th compared to other days and greater amount of necrosis on day 8 than on day 22. In the copaiba group, the number of gigantocytes increased compared to the days analyzed. Conclusion: Copaiba oil showed an improvement in the inflammatory response accelerating its beginning; however, did not affect the amount of abdominal adhesions or collagen fibers.

Neuroendocrine neoplasms (NENs) are a heterogeneous group of tumors with distinct morphological and biological manifestations, the liver being the main organ affected by its metastases. However, primary hepatic involvement is quite rare. Hepatic NENs can have a variety of radiological presentation forms and can therefore mimic other lesions, making their diagnosis challenging. Nonetheless, certain imaging aspects allow NENs to be included among the main differential diagnoses of hepatic lesions and can guide the search for an extrahepatic primary site when the probable diagnosis is metastases.

PURPOSE:To evaluate the effect of fasting on gastric emptying in mice. METHODS:Twenty-eight mice were distributed into three study groups: a normal group (N=4): normal standard animals; a total fasting group (N=12): subjected to food and water deprivation and a partial fasting group (N=12): subjected to food deprivation only.The fasting groups were subdivided into three subgroups of four animals each, according to the date of euthanasia: 24, 48 and 72 hours.Was analyzed: the gastric volume, degree of the gastric wall distention and the presence of food debris in gastrointestinal tract. RESULTS:The mean gastric volume was 1601 mm 3 in the normal group, 847 mm 3 in total fasting group and 997 mm 3 in partial fastinggroup. There was difference between the fasting groups in any analyzed period (p<0.05). Regarding the presence of food debris in the gastrointestinal tract and the degree of distension of the stomach, there was no difference between the groups that underwent total or partial fasting (p>0.05). CONCLUSION:Total fasting or only-solids deprivation does not induce gastric emptying in mice.

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Introduction: taking into consideration the technical complexity involved in liver transplantation, the anesthetic-surgical team training, through experimental surgery on animals, has been considered a fundamental step before clinical application. Objective: to describe the technique modification of liver harvesting in orthotopic transplantation in pigs. Methods: seven sows Sus escrofa were used. The animals were previously anesthetized and placed in trans-operative monitoring; followed by the harvest of the organ by means of a different sequence than the reported by the literature, in which the liver vessels are handled in a clockwise direction. Results: the average time of hepatic harvesting was 84.4 minutes and the time of each anatomical structure dissection was considered satisfactory. Conclusion: the technique of liver harvesting in orthotopic transplantation in pigs described is viable, effective and reproducible, enabling the training of professionals and academics.

In the Amazon rainforest region, Andiroba oil is an herbal oil that is widely used by the local population to treat several inflammatory diseases. The objective of this study was to test Andiroba oil as a mesh coating or by oral administration in a rat model of excisional abdominal wall defect as an alternative to modulate inflammatory response without impairing the ventral hernia repair. Thirty six animals were distributed into three groups (N=12). (1) In the control group (CONT), ventral hernia repair was done using polypropylene/polyglecaprone mesh. (2) In the Andiroba oil gavage group (AndG), animals were treated with the meshes and 0.63 ml/kg of Andiroba oil was given by oral administration for 7 days prior to the mesh placement. (3) In the Andiroba oil submersion group (AndS), animals were treated with meshes that were previously submersed in Andiroba oil. At the 7th, 14th, and 21st days, macroscopic and microscopic analyses were done. AndG had fewer adhesions, necrosis, and lymphocytes, as well as similar collagen fiber formation and fibrosis areas as CONT. AndS showed a higher number of macrophages, fibrosis area, and less collagen fiber formation. Oral administration of Andiroba oil modulated inflammatory response, reduced abdominal adhesion formation, and did not impair tissue healing.