Fasting does not induce gastric emptying in rats

Brito, Marcus Vinicius Henriques; Yasojima, Edson Yuzur; Teixeira, Renan Kleber Costa; Houat, Abdallah de Paula; Yamaki, Vítor Nagai; Costa, Felipe Lobato da Silva

doi:10.1590/s0102-865020150030000001

Cited by 7 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This could apply to the very early stages of formulation development, where the design is optimized according to the stability, release, and behavior in cell culture. In addition, different in vivo settings, even though assayed in a same animal model, such as administration route (oral gavage, intestinal injection, or in situ intestinal loop), use of anesthesia, fasting time, and the employment of grid floors to avoid coprophagy,168–170 may highly influence the actual in vivo conditions for delivery. Last but not least, already established differences in length, morphology, pH, fluid content and composition, motility, and histology between animal models and humans171–176 could be translated into experimental guidelines.…”

Section: Challenges To the Clinical Translation Of Oral Drug Deliverymentioning

confidence: 99%

Oral Delivery of Biologics for Precision Medicine

2019

View full text Add to dashboard Cite

Precision medicine has led to the identification of new biological drugs and targets for personalized treatments. A limitation of biological drugs relies on their difficulties for overcoming biological barriers. However, recent developments on drug delivery are opening new avenues that may soon make feasible the oral administration of biologics. In article number 1901935, María José Alonso and co‐workers provide an overview of the current situation, future prospects, barriers to clinical translation, and identified opportunities for advancement in this field.

show abstract

Section: Challenges To the Clinical Translation Of Oral Drug Deliverymentioning

confidence: 99%

Oral Delivery of Biologics for Precision Medicine

2019

View full text Add to dashboard Cite

show abstract

“…Although Britto et all believe that total fasting or only-solids deprivation does not induce gastric emptying in mice, a preoperative total fasting for 6-8 hours could reduce this risk. [9] Cannibalism immediately after surgery is another issue to be considered. The rats need to be separated a few days after the operation until the wound is closed.…”

Section: Discussionmentioning

confidence: 99%

Complications after sciatic nerve defect repair in rats

Andrei¹,

Marin²,

Giuglea³

2020

roneuro

View full text Add to dashboard Cite

Experimental microsurgery is a provocative field with great rewards. Nerve microsurgery is particularly challenging because the results of the operation can only be tardily observed. During this time frame, multiple complications can appear to the laboratory rats, which can influence the final results. For this reason, when experimenting with Wistar rats, one must be familiarized with the possible complications in order to know the suitable solutions for all the issues. Lack of information and henceforth lack of action might result in compromising the final data.

show abstract

“…Fasting prior or post-surgery was not performed in any of the groups, since mice are unable to vomit and to prevent from potentially lethal hypoglycemia. [22] All study mice were weighed before the surgical intervention.…”

Section: Surgical Proceduresmentioning

confidence: 99%

Experimental Intestinal Stenosis Alters Crohn’s Disease-Like Intestinal Inflammation in Ileitis-Prone Mice

et al. 2021

View full text Add to dashboard Cite

Background: Clinical observations indicate that mechanical factors contribute to the expression or recurrence of Crohn's disease. We investigated whether the creation of an intestinal stenosis could alter the severity of the expected Crohn-like ileitis, in a Crohn's disease animal model, the TNF Δare/+ mouse. Methods: Thirty-six, 6-weeks-old TNF Δare/+ mice, were divided into 3 intervention groups: triple suture, single suture and sham. In the terminal ileum, in the first group, a triple suture stenosis was created, whereas, in the second, a loose suture was placed. Same triple-suture stenosis was performed on twelve wild type mice. All animals were sacrificed at 6 weeks post-operatively and the ileum parts were evaluated histopathologically. A summative total ileitis score was applied in each sample using a bespoke semiquantitative histological scoring system for the Crohn-like changes. Results: The triple suture stenosis induced significant muscular hypertrophy proximal to interventional site which was more prominent in TNF Δare/+ than wild type mice. In triple suture group, the total ileitis score was significantly increased proximal to the intervention as compared to the single suture ( P : 0.004) and the sham groups ( P : 0.013). The total ileitis score distally, was unaffected, regardless of the experimental intervention. Intestinal stenosis did not induce intestinal inflammation in WT mice. Conclusions: The creation of a stenosis in the terminal ileum of TNF Δare/+ mice alters Crohn-like inflammation. We assume that mechanical forces, such as intraluminal pressure, may contribute as important co-factors to the pathophysiology of Crohn's disease in genetically predisposed subjects.

show abstract

Fasting does not induce gastric emptying in rats

Cited by 7 publications

References 20 publications

Oral Delivery of Biologics for Precision Medicine

Oral Delivery of Biologics for Precision Medicine

Complications after sciatic nerve defect repair in rats

Experimental Intestinal Stenosis Alters Crohn’s Disease-Like Intestinal Inflammation in Ileitis-Prone Mice

Contact Info

Product

Resources

About