We studied the effect of inhibition of mitochondrial voltage-dependent anion channels with DIDS on radiosensitivity and mitochondrial status of K562 leukemic cells. The number of apoptotic and necrotic cells, mitochondrial transmembrane potential, and mitochondrial mass were evaluated after irradiation of cells in doses of 4 and 12 Gy in the presence and absence of the inhibitor. Inhibition of mitochondrial voltage-dependent anion channels increased radiosensitivity of K562 cells by 50-70% and decreased both mitochondrial transmembrane potential and mitochondrial mass. Inhibitors of voltage-dependent anion channels are promising agents capable of improving the effectiveness of cancer radiotherapy.
The article is concerned with the study of main biological properties of bacteria B. trematum species. In this work the research results of their tinctorial, cultural and biochemical properties are shown, with the view to grounding for setup scheme of isolation and bacteriological test of stated microorganism. According to obtained data bacteria of the given species represents gram-negative, coccoid bacillus that are able to grow both on usual and differentially diagnostic mediums. Bacteria B. trematum grow in temperature range of 17-420С, optimal temperature of cultivation is 37˚С. It should be noticed that the most relevant medium for cultivation is bordetellagar It was established that bacteria grow on the sodium chloride mediums in the range of 3-5%. It was found that studied culture of bacteria B. trematum shows проявляет asugarlytic properties, has positive reaction on catalase, negative reaction on cytochrome oxidase and DNAse. Also during the research it was established that bacteria B. trematum doesn’t utilize urea and citrate, has weak proteolytic activity and doesn’t utilize a number of amino acids. Similar results were obtained when using the set Api 20 E and NEFERMtest 24. Additionally it was established that B. trematum doesn’t reconstruct nitrates to nitrites, doesn’t enzyme β-galactosidase, adrinin hydrolase, triptophane dyaminaze, lysine decarboxylase, ornithine decarboxylase and urea doesn’t utilize citrates, doesn’t produce H2S, indole and acetone, doesn’t oxidate sucrose, melibios, amigdalin . Research results represented in the article provide the basis of isolation scheme and identification of bacteria of given species.
Статья посвящена разработке дифференциально-диагностической среды для идентификации бактерий вида Bordetella petrii. Разработанная селективная среда позволяет выявить бактерии B. petrii в течении 48-72 часов. Разработанные питательные среды специфичны по отношению к бактериям-ассоциантам других видов и родов. Оптимальным составом среды является (на литр): глюконат натрия -20 г, гидролизат казеина-3 г, дрожжевой экстракт-1 г, калий фосфорнокислый однозамещенный -1г, бромтимоловый синий-0,08 г, агар бактериологический-15 г, цефалексин-0,02 г. Показатель чувствительности среды составляет около 100 микробных клеток.
The article is devoted to the study of proteomes of poorly studied representatives of genus Bordetella. According to the results of the study, molecular masses of and protein masses of bacteria of genus Bordetella were extracted. In the NCBI system, in accordance with the data obtained by comparing genomes of Bordetella genus, an in-silico analysis of the correspondence of annotated proteomes was performed and the idea was identified by proteome of these species.
The history of study of Aeromonas genus bacteria, which is more than 100 years, continues active development nowadays. The article presents molecular genetic characteristics of Aeromonas hydrophila F-43 UGSKhA bacteriophage. Genome-wide sequencing of the bacteriophage was carried out to determine potential genetic pathogenicity loci and it was found that the size of the bacteriophage genome was 36801 bp. while potential loci of pathogenicity were not revealed. According to results of bioinformational (proteomic) analysis on sequencing data of F43-UGSKhA bacteriophage, 46 potential proteins with a molecular weight of 4.6-137.6 kDa that have their localization in the phage genome were identified. Phylogenetic position of F43-UGSKhA bacteriophage was determined in the group annotated in the NCBI system. Annotated bacteriophage Aeh1, which is active against bacteria Aeromonas hydrophila, is the closest bacteriophage in phylogenetic position of complete genome and most potential phage proteins. As a result of the studies, a linear bacteriophage DNA map was compiled. In accordance with major analogues, gene expression products were determined. Also, gene products were identified that do not have clearly defined functional characteristics, the so-called hypothetical proteins that do not have an analogy in the annotated genomes of bacteriophages that are active against the studied bacterial type. Bioinformation analysis of correspondence of open reading frames (ORF) with sequencing data of the studied bacteriophage is presented. According to the results of studies, no pathogenicity loci were detected in the genome of F-43 of UGSKhA Aeromonas hydrophila bacteriophage.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.