Molecular-genetic characteristics of Bacillus cereus FBC-28 UGSKHA bacilliophage

Feoktistova, N.A.; Vasilyev, D.А.; Mastilenko, A.V.; Suldina, E.V.

doi:10.18286/1816-4501-2018-2-216-222

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“…По литературным данным бактерии Bacillus cereus входят в состав основных контаминатов животного и растительного пищевого сырья, так как являются почвенными спорообразующими сапрофитами и при несоблюдении санитарного-гигиенических параметров переработки, высокой температурной устойчивости и солетолерантности могут стать причиной пищевых отравлений и при употреблении готовых кулинарных блюд [6][7][8][9].…”

Section: Introductionunclassified

Results of proteomic analysis of bacillus cereus FBc - 28 UGSKHA bacteriophage

Feoktistova¹,

Merchina²,

Mastilenko³

2018

Vestnik of Ulyanovsk SAA

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The article presents results of the proteomic analysis of Bacillus cereus FBc-28 UGSKhA bacteriophage (study of the quantitative composition, isoelectric point of proteins, molecular weight) isolated from environmental objects (soil test), which is of interest as a component of an experimental phage biological compound for food treatment to reduce quantitative parametres of microbial contamination. The resources of SnapGeneViewerv.4.1.7 and ExPasy (https:// web.expasy.org). were used in the experiments. As a result of the research, data of proteomic analysis were obtained on the basis of a previously performed sequence. It was established that the qualitative composition of the proteins of FBc-28 UGSKhA bacteriophage corresponds to those of the annotated analogs, has a clear homology of the nucleotide and amino acid sets. When analyzing the proteome of FBc-28 UGSKhA bacteriophage and, accordingly, the sequencing of its nucleic acid, 60 proteins with molecular masses from 5.7 to 132.3 kDa were detected. The histogram of the distribution of the protein composition of Bacillus cereus of FBc-28 UGSKhA at the isoelectric point (pI) captures the values in a wide range of 4.2-12.1. The obtained data on the proteome of FBc-28 UGSKhA bacteriophage specific for Bacillus cereus brings us closer to the development of phage preparations of a new generation intended to decontaminate food raw materials, which have high specificity and a wide range of lytic action within the bacterial species, and complying with biosafety standards.

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Section: Introductionunclassified