We studied the effect of inhibition of mitochondrial voltage-dependent anion channels with DIDS on radiosensitivity and mitochondrial status of K562 leukemic cells. The number of apoptotic and necrotic cells, mitochondrial transmembrane potential, and mitochondrial mass were evaluated after irradiation of cells in doses of 4 and 12 Gy in the presence and absence of the inhibitor. Inhibition of mitochondrial voltage-dependent anion channels increased radiosensitivity of K562 cells by 50-70% and decreased both mitochondrial transmembrane potential and mitochondrial mass. Inhibitors of voltage-dependent anion channels are promising agents capable of improving the effectiveness of cancer radiotherapy.
The article is concerned with the study of main biological properties of bacteria B. trematum species. In this work the research results of their tinctorial, cultural and biochemical properties are shown, with the view to grounding for setup scheme of isolation and bacteriological test of stated microorganism. According to obtained data bacteria of the given species represents gram-negative, coccoid bacillus that are able to grow both on usual and differentially diagnostic mediums. Bacteria B. trematum grow in temperature range of 17-420С, optimal temperature of cultivation is 37˚С. It should be noticed that the most relevant medium for cultivation is bordetellagar It was established that bacteria grow on the sodium chloride mediums in the range of 3-5%. It was found that studied culture of bacteria B. trematum shows проявляет asugarlytic properties, has positive reaction on catalase, negative reaction on cytochrome oxidase and DNAse. Also during the research it was established that bacteria B. trematum doesn’t utilize urea and citrate, has weak proteolytic activity and doesn’t utilize a number of amino acids. Similar results were obtained when using the set Api 20 E and NEFERMtest 24. Additionally it was established that B. trematum doesn’t reconstruct nitrates to nitrites, doesn’t enzyme β-galactosidase, adrinin hydrolase, triptophane dyaminaze, lysine decarboxylase, ornithine decarboxylase and urea doesn’t utilize citrates, doesn’t produce H2S, indole and acetone, doesn’t oxidate sucrose, melibios, amigdalin . Research results represented in the article provide the basis of isolation scheme and identification of bacteria of given species.