Molecular genetic characteristics of F-43 UGSKHA Aeromonas hydrophila bacteriophage

Abstract

The history of study of Aeromonas genus bacteria, which is more than 100 years, continues active development nowadays. The article presents molecular genetic characteristics of Aeromonas hydrophila F-43 UGSKhA bacteriophage. Genome-wide sequencing of the bacteriophage was carried out to determine potential genetic pathogenicity loci and it was found that the size of the bacteriophage genome was 36801 bp. while potential loci of pathogenicity were not revealed. According to results of bioinformational (proteomic) analysis on sequencing data of F43-UGSKhA bacteriophage, 46 potential proteins with a molecular weight of 4.6-137.6 kDa that have their localization in the phage genome were identified. Phylogenetic position of F43-UGSKhA bacteriophage was determined in the group annotated in the NCBI system. Annotated bacteriophage Aeh1, which is active against bacteria Aeromonas hydrophila, is the closest bacteriophage in phylogenetic position of complete genome and most potential phage proteins. As a result of the studies, a linear bacteriophage DNA map was compiled. In accordance with major analogues, gene expression products were determined. Also, gene products were identified that do not have clearly defined functional characteristics, the so-called hypothetical proteins that do not have an analogy in the annotated genomes of bacteriophages that are active against the studied bacterial type. Bioinformation analysis of correspondence of open reading frames (ORF) with sequencing data of the studied bacteriophage is presented. According to the results of studies, no pathogenicity loci were detected in the genome of F-43 of UGSKhA Aeromonas hydrophila bacteriophage.