Viabilidade e atividade antioxidante de sementes de genótipos de feijão-miúdo submetidos ao estresse salino

Several factors affect the quality of soybean seeds, including the mineral nutrition of plants. Manganese (Mn) is an important nutrient because it has as one of its functions the enzyme activation. The aim of this study was to evaluate enzyme activity in seeds of soybean cultivars produced with foliar application of different doses of Mn. The experiment was conducted at the Universidade Federal de Lavras (UFLA) (Federal University of Lavras) in randomized blocks with three replications and 4 x 4 x 2 factorial arrangement consisting of four soybean cultivars, two conventional and its genetically modified RR derived (BRS Celeste and BRS Baliza RR; BRSGO Jataí and BRS Silvânia RR), four doses of Mn via foliar application (0; 200; 400 and 600 g Mn ha -1 ) and two stages of application (R 1 or R 3 ). In the seeds, the expressions of the enzymes esterase (EST), malate dehydrogenase (MDH), alcohol dehydrogenase (ADH), superoxide dismutase (SOD), peroxidase (PRX) and isocitratelyase (ICL) were determined. For evaluation of physiological quality, the germination test and emergence speed index (ESI) were performed. The Mn content in the seeds was also determined. The expression of the enzymes EST, SOD, PRX and ICL in soybean seeds are affected by foliar application of Mn, regardless of the stages of application. In the seeds of the cultivars that showed better physiological quality, Celeste and Baliza RR, greater expressions of the enzymes ADH and ICL and lower expressions of MDH and of PRX were observed.Index terms: Enzymes, Glycine max, quality, micronutrients. RESUMODiversos fatores afetam a qualidade das sementes de soja, dentre eles a nutrição mineral das plantas. O manganês (Mn) é um nutriente importante por ter como uma de suas funções a ativação enzimática. O objetivo, neste trabalho, foi avaliar a atividade enzimática em sementes de cultivares de soja, produzidas com aplicação foliar de diferentes doses de Mn. O experimento foi conduzido na Universidade Federal de Lavras (UFLA), em blocos casualizados, com três repetições e esquema fatorial 4 x 4 x 2, sendo quatro cultivares de soja, duas convencionais e suas derivadas transgênicas RR (BRS Celeste e BRS Baliza RR; BRSGO Jataí e BRS Silvânia RR), quatro doses de Mn via foliar (0; 200; 400 e 600 g Mn ha -1 ) e dois estádios de aplicação (R 1 ou R 3 ). Nas sementes, foram determinadas as expressões das enzimas esterase (EST), malato desidrogenase (MDH), álcool desidrogenase (ADH), superóxido dismutase (SOD), peroxidase (PRX) e isocitrato liase (ICL). Para avaliação da qualidade fisiológica foram realizados os testes de Germinação e de Índice de velocidade de emergência (IVE). Determinou-se, também, o teor de Mn nas sementes. A expressão das enzimas EST, SOD, PRX e ICL em sementes de soja são influenciadas pela aplicação de Mn via foliar, independente do estádio de aplicação. Nas sementes das cultivares, as quais apresentaram melhor qualidade fisiológica, Celeste e Baliza RR, foram constatadas maiores expressões das enzimas ADH e ICL e menores expressões de ...

show abstract

“…•-) into H 2 O 2 and O 2 (Deuner et al, 2011) and the peroxidases (PRX) are specialized in removal of H 2 O 2 (Moller, Jensen;Hansson, 2007).…”

Section: Introductionmentioning

confidence: 99%

Enzyme activity in soybean seeds produced under foliar application of manganese

et al. 2014

View full text Add to dashboard Cite

show abstract

“…In general, there was an increase in APX and CAT activity in all varieties when subjected to 100 mM NaCl. Deuner et al (2011) observed that seeds of cowpea genotypes maintain high germination and APX and CAT activity until the NaCl concentration of 100 mM. The variety RB867515 showed higher action of these enzymes in the repair of damages caused by salinity, due to the significant increase in APX and CAT activity, in comparison to the results for RB92579 and RB962962.…”

Section: Bmentioning

confidence: 83%

Enzymatic activity of three sugarcane varieties under salt stress

Carvalho

Corrêa

Carvalho

et al. 2016

Rev. bras. eng. agríc. ambient.

View full text Add to dashboard Cite

A B S T R A C TSugarcane is a species of great economic importance to Brazil. The study of enzymatic activity has helped to identify varieties of sugarcane tolerant to salt stress, for planting in Brazilian semiarid regions, where there is the occurrence of saline soils. The objective of this study was to evaluate the enzymatic activity of three varieties of sugarcane subjected to stress caused by salinity. The experiment was conducted in a greenhouse at the Federal Rural University of Pernambuco, in a completely randomized design with four replicates. The leaf blade samples were collected for analysis of soluble proteins and determination of the activity of catalase and ascorbate peroxidase. The protein content was higher for RB962962 and lower for the varieties RB92579 and RB867515. However, in RB867515, there was an increase in the activity of ascorbate peroxidase and catalase, while the varieties RB92579 and RB962962 showed a reduction in the activity of these enzymes. These results indicate that the variety RB867515 has a more efficient mechanism in plant protection against the action of reactive oxygen species.Atividade enzimática de três variedades de cana-de-açúcar sob estresse salino R E S U M O A cana-de-açúcar é uma espécie de grande importância econômica para o Brasil. O estudo da atividade enzimática tem ajudado a identificar variedades de cana-de-açúcar tolerantes ao estresse salino para plantio nas regiões semiáridas brasileiras nas quais ocorre incidência de solos salinizados. Objetivou-se, neste estudo, avaliar a atividade enzimática de três variedades de cana-de-açúcar submetidas ao estresse causado pela salinidade. O experimento foi conduzido em casa de vegetação na Universidade Federal Rural de Pernambuco. Foi feito um delineamento inteiramente casualizado, com quatro repetições. Foram coletadas amostras do limbo foliar para análise das proteínas solúveis e determinação da atividade das enzimas catálase e ascorbato peroxidase. O teor de proteínas foi maior para a variedade RB962962 and menor para as variedades RB92579 e RB867515; entretanto se percebeu, na variedade RB867515, aumento da atividade da ascorbato peroxidase e da catálase enquanto que nas variedades RB92579 e RB962962 ocorreu uma redução da atividade dessas enzimas cujos resultados indicam que a variedade RB867515 apresenta um mecanismo mais eficiente na proteção da planta contra a ação de espécies reativas de oxigênio.

show abstract

“…At the end of the germination test we collected samples of 400 mg of fresh plant material, which were macerated with 10% of polyvinylpolypyrrolidone, to prevent oxidation, after which were homogenized with 1.5 mL of an extraction buffer, pH 7.8, comprising potassium phosphate 100 mM, ethylenediaminetetraacetic acid (EDTA) 0.1 mM, and ascorbic acid 20 mM. The material was centrifuged at 12,000 g for 20 min at 4°C, an adaptation of the methodology described by Deuner et al (2011). The supernatant was collected to determine the activity of enzymes and to quantify the proteins by the Bradford method (1976).…”

Section: Methodsmentioning

confidence: 99%

“…•− into H 2 O 2 and O 2 ; catalase (CAT); and ascorbate peroxidase (APX), which can cleave H 2 O 2 into H 2 O and O 2 (Deuner et al 2011). However, this regulation can be ineffective if the stress is severe enough to considerably increase the production of ROS, which can cause a cascade of events: peroxidation of lipids, degradation of membranes, and cell death (Pacheco et al 2007).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Salt stress: antioxidant activity as a physiological adaptation of onion cultivars

et al. 2013

View full text Add to dashboard Cite

The germination and development of several plant species can be limited when those plants are grown in soils with high salinity, which reduces seedling viability and vigor, as well as activating the antioxidant defense system. The aim of this study was to evaluate germination, initial growth and activity of antioxidant enzymes (superoxide dismutase, catalase and ascorbate peroxidase) in seedlings of three onion cultivars (Madrugada, Fepagro 27 and Petroline) exposed to different concentrations of NaCl (0, 40, 80, 120 and 160 mM). Seedlings were evaluated for viability, vigor and antioxidant enzyme activity. The experimental procedures were completely randomized in 3 × 5 factorial design, with each treatment performed in triplicate, at a significance level of 5%. For all cultivars, viability and vigor decreased in parallel with increasing NaCl concentrations, whereas antioxidant enzyme activity increased, and one cultivar (Madrugada) showed less salt tolerance than did the others. We conclude that high NaCl concentrations have a negative effect in the physiological quality of onion seeds, resulting in lower seedling growth rates and increased antioxidant enzyme activity, where Fepagro 27 and Petroline cultivars were more tolerant to salt stress than 'Madrugada' .

show abstract

Viabilidade e atividade antioxidante de sementes de genótipos de feijão-miúdo submetidos ao estresse salino

Cited by 48 publications

References 31 publications

Enzyme activity in soybean seeds produced under foliar application of manganese

Enzyme activity in soybean seeds produced under foliar application of manganese

Enzymatic activity of three sugarcane varieties under salt stress

Salt stress: antioxidant activity as a physiological adaptation of onion cultivars

Contact Info

Product

Resources

About