Viabilidade Do Pólen Em Variedades De Laranja Doce

Domingues, Edson Tobias; Neto, Augusto Tulmann; Sobrinho, Joaquim Teófilo

doi:10.1590/s0103-90161999000200002

Cited by 10 publications

(8 citation statements)

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“…There are several methods for the evaluation of pollen viability, e.g., evaluation by acetic carmine staining (Domingues et al 1999), incubation in Baker solution (Oliveira et al 2001) or the germination test in culture medium Raseira 2006, Pio et al 2007). The method of assessing the viability of pollen through germination in culture medium in vitro is practical and accurate (Einhardt et al 2006).…”

Section: Introductionmentioning

confidence: 99%

In vitro viability and preservation of pollen grain of kiwi (Actinidia chinensis var. deliciosa (A. Chev.) A. Chev)

Borghezan

Clauman

Steinmacher³

et al. 2011

Crop Breed. Appl. Biotechnol.

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-Kiwi is a dioecious plant species, requiring cross pollination for fruit production. The objective of this study was to evaluate the in vitro viability and shelf life of pollen grains of two kiwi varieties. Flowers of the cultivars Matua and Tomuri were collected and the pollen germinated in vitro, in culture medium containing agar (1 %), sucrose (0, 5, 10, 20 and 40 %) and boric acid (0 and 50 mg L -1 H 3 BO 3 ). Pollen grains were stored in a BOD incubator (25.0 °C), refrigerator (4.0 °C), freezer (-18.0 °C) and in liquid N 2 (-196.0 °C), and evaluated after 0, 40, 120, 240 and 365

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Section: Introductionmentioning

confidence: 99%

In vitro viability and preservation of pollen grain of kiwi (Actinidia chinensis var. deliciosa (A. Chev.) A. Chev)

Borghezan

Clauman

Steinmacher³

et al. 2011

Crop Breed. Appl. Biotechnol.

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“…Entretanto, de acordo com outros autores, esses testes estão associados apenas à detecção de substâncias constituintes dos grãos de pólen, como amido e lipídios, que podem estar presentes tanto em grãos de pólen maduros como nos abortados (King 1960, Beyhaut 1988, Rodriguez-Riano & Dafni 2000. Corantes, como o carmim acético e a solução de Alexander, são também utilizados como indicativos de viabilidade polínica (Mulugeta et al 1994, Domingues et al 1999, Rigamoto & Tyagi 2002, porém para Báez et al (2002) e Pline et al (2002) estes testes refletem somente a integridade de estruturas celulares, como núcleo e membrana plasmática.…”

Section: Introductionunclassified

Viabilidade polínica de Carica papaya L.: uma comparação metodológica

Munhoz¹,

Luz²,

Filho³

et al. 2008

Rev. bras. Bot.

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-(Pollen viability of Carica papaya L.: a methodological comparison). Pollen viability of Carica papaya L. 'Sunrise Solo' cultivar was estimated using in vitro germination and pollen stain tests. Two culture media described in the literature as efficient for pollen in vitro germination of C. papaya were used. Germination was higher in the media without essential elements but with a higher concentration of agar (65% compared with 51.5%). These results were compared with viability estimates based on staining pollen. The five stains used to test for pollen viability were: 2,3,5-triphenyl tetrazolium chloride -TTC, Alexander's stain, acetocarmine, iodine-potassium iodide and Sudan IV. Pollen viability estimated with TTC was 67.5% and similar to germination results providing a reliable estimate of in vitro germination. The four other stains overestimated pollen viability (> 90%), and their use should be restricted to determining cell constituents, and pollen grain integrity.

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“…Cavalcante et al (2000) found variances of 9 to 98% in the estimation of the viability of the pollen of tangeleiros, using propionic carmine as dye. Domingues et al (1999) found viability of up to 78.2% in pollen in the 'Common Caipira' variety of oranges. Brugnara et al (2011) reported values varying from 42% to 70% of viability in mandarins (Citrus sp.)…”

Section: Couto (2002)mentioning

confidence: 96%

BIOLOGY OF POLLINATION OF Citrus sinensis VARIETY ‘PERA RIO’

2017

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ABSTRACT-Aspects related to the floral biology of Citrus sinensis 'Pera Rio' variety were studied in the present work aiming to obtain information about the pollination ecology in the local agriculture. Studies of flowering, anthesis, pollen / ovule ratio, stigmatic receptivity, pollen viability, nectar characterization and floral visitors were carried out. From the data studied, the following information was obtained: C. sinensis variety 'Pera Rio' flourishes in two annual periods (dry and rainy), the anthesis occurs from 9:00 AM with duration of 24 hours, during which period stigma receptivity, pollen viability and nectar secretion are highly significant, demonstrating that the species also possesses characteristics of allogenic plants. Apis mellifera was a floral visitor, with a greater number of individuals with a Relative Frequency (RF) of 51.1%. Melipona scutellaris obtained second place in visits with RR = 23.6%; Followed by Trigona spinipes with FR = 17.7%. The two seasons (dry and rainy) presented high similarity (Morisita index = 0.64). C. sinensis has floral biology favorable to cross pollination and bees A. mellifera and M. scutellaris are potential pollinators of this fruit in the conditions in the region of the Recôncavo Baiano. The reproductive system of C. sinensis is mixed, being favorable to entomophilic pollination. Index terms: Apoidea, citricultura; pollination; bee population, orange. BIOLOGIA DA POLINIZAÇÃO DE Citrus sinensis VARIEDADE 'PERA RIO'RESUMO-Aspectos relacionados à biologia floral de Citrus sinensis var Pera Rio foram estudados no presente trabalho, com o objetivo de obter informações da ecologia da polinização na agricultura local. Foram realizados estudos da floração, antese, razão pólen/óvulo, receptividade estigmática, viabilidade polínica, caracterização do néctar e visitantes florais. A partir dos dados estudados, foram obtidas as seguintes informações: C. sinensis var Pera Rio floresce em dois períodos anuais (seco e chuvoso), a antese ocorre a partir das 9:00 da manhã com duração de 24 horas, período pelo qual a receptividade do estigma, viabilidade polínica e a secreção de néctar são altamente significativos demonstrando que a espécie também possui características de plantas alogâmicas. Apis mellifera se destacou como visitante floral, com um maior número de indivíduos com Frequência Relativa (FR) de 51,1%. Melipona scutellaris obteve segundo lugar nas visitas com FR = 23,6%; seguida por Trigona spinipes com FR = 17,7%. As duas estações (seca e chuvosa) apresentaram similaridade alta (índice de Morisita = 0,64). C. sinensis possui biologia floral favorável à polinização cruzada e as abelhas A. mellifera e M. scutellaris são potenciais polinizadores dessa fruteira nas condições do Recôncavo Baiano. O sistema reprodutivo de C. sinensis é misto, sendo favorável à polinização entomófila. Termos para indexação: Apoidea, citricultura; polinização; população de abelhas, laranja.

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Viabilidade Do Pólen Em Variedades De Laranja Doce

Cited by 10 publications

References 1 publication

In vitro viability and preservation of pollen grain of kiwi (Actinidia chinensis var. deliciosa (A. Chev.) A. Chev)

In vitro viability and preservation of pollen grain of kiwi (Actinidia chinensis var. deliciosa (A. Chev.) A. Chev)

Viabilidade polínica de Carica papaya L.: uma comparação metodológica

BIOLOGY OF POLLINATION OF Citrus sinensis VARIETY ‘PERA RIO’

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