A label-free electrochemical biosensor has been developed using Zinc Oxide nano owers (ZnONFs) for the detection of Uric acid. ZnONFs have been synthesized by hydrothermal process and characterized with several techniques such as Ultraviolet-Visible spectroscopy, Fourier Transform Infrared Spectroscopy (FT-IR) study, X-ray diffraction study, Raman spectroscopy, Scanning Electron Microscopy and High-Resolution Transmission Electron Microscopy (HR-TEM) and electrochemical analyser to con rms the formation of nano owers and fabrication of electrode and bioelectrodes for uric acid detection. Pure and uniform needle owers and deposited onto Indium Tin Oxide (ITO) substrate through electrophoretic deposition technique. Further, electrochemical studies have been performed with immobilized enzymatic bioelectrode followed by various uric acid concentrations. It has been found that the fabricated biosensor shows high sensitivity (10.38 µA/ mg/mL /cm2) and a limit of detection of 0.13 mg/mL in the range of 0.005 to 1.0 mg/mL. This study demonstrates the potential use of ZnONFs for the construction of overly sensitive biosensors for Uric acid detection.