Fabrication of Electrochemical Biosensor Using Zinc Oxide Nanoflowers for the Detection of Uric Acid

Abstract

A label-free electrochemical biosensor has been developed using Zinc Oxide nano owers (ZnONFs) for the detection of Uric acid. ZnONFs have been synthesized by hydrothermal process and characterized with several techniques such as Ultraviolet-Visible spectroscopy, Fourier Transform Infrared Spectroscopy (FT-IR) study, X-ray diffraction study, Raman spectroscopy, Scanning Electron Microscopy and High-Resolution Transmission Electron Microscopy (HR-TEM) and electrochemical analyser to con rms the formation of nano owers and fabrication of electrode and bioelectrodes for uric acid detection. Pure and uniform needle owers and deposited onto Indium Tin Oxide (ITO) substrate through electrophoretic deposition technique. Further, electrochemical studies have been performed with immobilized enzymatic bioelectrode followed by various uric acid concentrations. It has been found that the fabricated biosensor shows high sensitivity (10.38 µA/ mg/mL /cm2) and a limit of detection of 0.13 mg/mL in the range of 0.005 to 1.0 mg/mL. This study demonstrates the potential use of ZnONFs for the construction of overly sensitive biosensors for Uric acid detection.

Uricase Crowding via Polyelectrolyte Layers Coacervation for Carbon Fiber-Based Electrochemical Detection of Uric Acid

“…The obtained linear range, sensitivity, limit of detection, and Michaelis–Menten constant values of the fabricated sensor show its competitiveness with analogous enzymatic uric acid electrochemical sensors based on screen-printed electrodes or metal substrates [ 29 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 ], the main characteristics of which are listed in Table 2 , as well as non-enzymatic sensors [ 56 ]. Unfortunately, sensor performance data for the electrodes modified with polyelectrolyte multilayers with alternating uricase layers [ 54 ] were insufficient for a direct comparison with the sensor in this work, but our fabricated sensor benefits from a lower consumption of uricase enzyme (one layer in this work vs. ten layers in [ 54 ]) and a less expensive substrate (CFE vs. Pt).…”

Surface modified ZnONFs with Oleic acid to fabricate nanobiosensors for uric acid detection

“…Subsequently, 1 g of zinc oxide nanoflower powder (powder A) [32] was added to the solution, and the mixture was allowed to react for one hour at 60 °C. Afterwards, the particles were separated using centrifugal force and thoroughly washed with toluene, rinsed three times.…”

Amelioration in nanobiosensors for the control of plant diseases: current status and future challenges

“…It is thus important to note that the majority of crop losses are due to biotic and abiotic stresses. It is thought that 20%-40% of crop losses are due to biotic factors including pest attack, pathogen invasion, herbicides, and insect attack (Dutta et al, 2022;Munir et al, 2023). Therefore, a major challenge is the management of plant diseases against biotic factors.…”

“…Moreover, nanoparticles can ameliorate plant genetic health and make plants tolerant to drought, heat, and salt stress which might be due to the height-surface-to-volume ratio of nanoparticles that increases their reactivity and possible biochemical activity (Khan and Rizvi, 2014;El Moneim et al, 2021). Nanomaterials are useful for researchers and scientists because of their ability as carriers, and their small size, ease of transport, and active surface area (Hazarika et al, 2021;Dutta et al, 2022). Ligand-assisted nanoclusters can be used to detect contaminants in agricultural products and offer a range of applications that leverage their unique properties.…”