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Due to the similar electrochemical properties to graphene oxide (GO), graphene quantum dots (GQDs) are considered as a highly potential candidate for designing an electrochemical biosensor. In this report, GQDs were synthesized having an average diameter of 7 nm and utilized for the fabrication of an electrochemical immunosensor for the detection of food toxin, aflatoxin B (AFB). An electrophoretic deposition technique was utilized to deposit the chemically synthesized GQDs onto indium tin oxide (ITO)-coated glass substrate. Further, the monoclonal antibodies of AFB were covalently immobilized onto deposited electrode GQDs/ITO using EDC-NHS as a crosslinker. The structural and morphological studies of GQDs and conjugated anti-AFB with GQDs have been investigated using UV-visible spectroscopy, photoluminescence spectroscopy, Raman spectroscopy, transmission electron microscopy, scanning electron microscopy techniques, etc. The electrochemical impedance spectroscopy and cyclic voltammetry measurements were carried out for electrical characterization and biosensing studies. This simple monodisperse GQDs-based platform yields heterogeneous electron transfer (97.63 × 10 cm s), the time constant (0.005 s) resulting in improved biosensing performance. The electrochemical immunosensor shows high sensitivity 213.88 Ω (ng mL) cm. The limit of detection for standard samples and contaminated maize samples was found to be 0.03 ng mL and 0.05 ng g, respectively, which is lower than the maximum acceptable limit according to the European Union. This result indicates its potential application for aflatoxin B detection in food contents. Graphical abstract ᅟ.
Decoration of graphene quantum dots (GQDs) on molybdenum disulfide (MoS 2 ) nanosheets serves as an active electrode material which enhances the electrochemical performance of the analyte detection system. Herein, ionic surfactant cetyltrimethylammonium bromide (CTAB)-exfoliated MoS 2 nanosheets decorated with GQD material are used to construct an electrochemical biosensor for aflatoxin B1 (AFB1) detection. An antibody of AFB1 (aAFB1) was immobilized on the electrophoretically deposited MoS 2 @GQDs film on the indium tin oxide (ITO)-coated glass surface using a crosslinker for the fabrication of the biosensor. The immunosensing study investigated by the electrochemical method revealed a signal response in the range of 0.1 to 3.0 ng/mL AFB1 concentration with a detection limit of 0.09 ng/mL. Also, electrochemical parameters such as diffusion coefficient and heterogeneous electron transfer (HET) were calculated and found to be 1.67 × 10 −5 cm 2 /s and 2 × 10 −5 cm/s, respectively. The effective conjugation of MoS 2 @GQDs that provides abundant exposed edge sites, large surface area, improved electrical conductivity, and electrocatalytic activity has led to an excellent biosensing performance with enhanced electrochemical parameters. Validation of the fabricated immunosensor was performed in a spiked maize sample, and a good percentage of recoveries within an acceptable range were obtained (80.2 to 98.3%).
An electrochemical biosensor was developed to determine formaldehyde (HCHO) adulteration commonly found in food. The current responses of various electrodes based on multiwalled carbon nanotubes (CNTs) and synthesized nanocomposite (CNT-Fe 3 O 4) were measured using cyclic voltammetry. The nanocomposite based biosensor shows comparatively high sensitivity (527 lA mg/L-1 cm-2), low detection limit (0.05 mg/L) in linear detection range 0.05-0.5 mg/L for formaldehyde detection using formaldehyde dehydrogenase (FDH) enzyme. In real sample analysis, the low obtained RSD values (less than 1.79) and good recovery rates (more than 90%) signify an efficient and precise sensor for the selective quantification of formaldehyde in orange juice. The developed biosensor has future implications for determining formaldehyde adulteration in citrus fruit juices and other liquid foods in agri-food chain to further resolve global food safety concerns, control unethical business practices of adulteration and reduce the widespread food borne illness outbreaks.
This work reports the development of an electrical and optical biosensing for label-free detection of Aflatoxin B 1 (AFB 1) using gold (Au) nanobipyramids (NBPs). AuNBPs were synthesized through a two-step seed-mediated growth process followed by an exchange of capping agent from surfactant to lipoic acid. Pure and monodispersed AuNBPs of 70 nm base length were obtained and deposited on indium tin oxide (ITO)-coated glass substrate modified with self-assembled (3-Aminopropyl) triethoxysilane (APTES) film. The characterization of the obtained surfaces using spectroscopy, microscopy and diffractometry confirms the formation of AuNBPs, the conjugation to ITO electrode substrate and the immobilization of anti-AFB 1 antibodies. AuNBPs modified ITO substrates were used for both electrochemical and Surface Plasmon Resonance biosensing studies. Localized Surface Plasmon Resonance (LSPR) local field enhancement was demonstrated. SPR based AFB 1 detection was found to be linear in the 0.1 to 500 nM range with a limit of detection of 0.4 nM, whereas, impedimetric AFB 1 detection was shown to be linear in the 0.1 to 25 nM range with a limit of detection of 0.1 nM. The practical utility of the impedimetric sensor was tested in spiked maize samples and 95-100% recovery percentage were found together with low relative standard deviation, proof of the robustness of this AFB 1 sensor.
For the management and prevention of many chronic and acute diseases, the rapid quantification of toxicity in food and feed products have become a significant concern. Technology advancements in the area of biosensors, bioelectronics, miniaturization techniques, and microfluidics have shown a significant impact than conventional methods which have given a boost to improve the sensing performance towards food analyte detection. In this article, recent literature of Aflatoxin B 1 (AFB 1 ), worldwide permissible limits, major outbreaks and severe impact on healthy life have been discussed. An improvement achieved in detection range, limit of detection, shelf-life of the biosensor by integrated dimensional nanomaterials such as zero-dimension, one-dimension and two-dimension for AFB 1 detection using electrical and optical transduction mechanism has been summarized. A critical overview of the latest trends using paper-based and micro-spotted array integrated with the anisotropic shape of nanomaterials, portable microfluidic devices have also been described together with future perspectives for further advancements.
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