Ultraviolet Hyperspectral Interferometric Microscopy

Ojaghi, Ashkan; Fay, Meredith E.; Lam, Wilbur A.; Robles, Francisco E.

doi:10.1038/s41598-018-28208-0

Cited by 35 publications

(39 citation statements)

References 19 publications

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“…There is an increasing interest in analyzing both the absorptive and dispersive optical properties of biological samples in order to gain a more detailed understanding of their physical and chemical composition for basic research and the study of disease [1][2][3][4][5][6]. (Dispersion refers to changes in the real part of the refractive index as a function of wavelength).…”

Section: Introductionmentioning

confidence: 99%

“…In this work we present, to the best of our knowledge, the first study of the unique absorptive and dispersive signatures of several physiologically important biomolecules in the deep-UV region of the spectrum. Our approach is based on a recently developed ultraviolet hyperspectral interferometry (UHI) setup [1], which enables deep-UV, wide-band, highresolution, and high sensitivity spectroscopic measurements of the refractive index and molar extinction coefficient of biomolecules. UHI microscopy was developed to provide quantitative spectral information of endogenous molecules with subcellular spatial resolution and sensitivity to nanometer-scaled structures for label-free molecular imaging of live cells and thin tissue samples [1].…”

Section: Introductionmentioning

confidence: 99%

“…Our approach is based on a recently developed ultraviolet hyperspectral interferometry (UHI) setup [1], which enables deep-UV, wide-band, highresolution, and high sensitivity spectroscopic measurements of the refractive index and molar extinction coefficient of biomolecules. UHI microscopy was developed to provide quantitative spectral information of endogenous molecules with subcellular spatial resolution and sensitivity to nanometer-scaled structures for label-free molecular imaging of live cells and thin tissue samples [1]. In this work we analyze the deep-UV absorptive and dispersive properties of biomolecules that play an important role in the structure and function of biological systems to gain a better understanding of their spectral properties.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Deep UV dispersion and absorption spectroscopy of biomolecules

Soltani

Ojaghi

Robles

2019

Biomed. Opt. Express

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Owing to the high precision and sensitivity of optical systems, there is an increasing demand for optical methods that quantitatively characterize the physical and chemical properties of biological samples. Information extracted from such quantitative methods, through phase and/or amplitude variations of light, can be crucial in the diagnosis, treatment and study of disease. In this work we apply a recently developed quantitative method, called ultraviolet hyperspectral interferometry (UHI), to characterize the dispersion and absorbing properties of various important biomolecules. Our system consists of (1) a broadband light source that spans from the deep-UV to the visible region of the spectrum, and (2) a Mach-Zehnder interferometer to gain access to complex optical properties. We apply this method to characterize (and tabulate) the dispersive and absorptive properties of hemoglobin, beta nicotinamide adenine dinucleotide (NAD), flavin adenine dinucleotide (FAD), elastin, collagen, cytochrome c, tryptophan and DNA. Our results shed new light on the complex properties of important biomolecules.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Deep UV dispersion and absorption spectroscopy of biomolecules

Soltani

Ojaghi

Robles

2019

Biomed. Opt. Express

Self Cite

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“…Since the technology is fairly new, there are no explicit quantum‐enhanced imaging experiments yet. Nevertheless, there are plenty of experimental applications of low light imaging at high quality level …”

Section: Quantum Imaging Device Developmentmentioning

confidence: 99%

“…Nevertheless, there are plenty of experimental applications of low light imaging at high quality level. [177][178][179]…”

Section: Scientific Complementary Metal-oxide Semiconductormentioning

confidence: 99%

Perspectives for Applications of Quantum Imaging

Basset

Setzpfandt

Steinlechner

et al. 2019

Laser & Photonics Reviews

110

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Quantum imaging is a multifaceted field of research that promises highly efficient imaging in extreme spectral ranges as well as ultralow‐light microscopy. Since the first proof‐of‐concept experiments over 30 years ago, the field has evolved from highly fascinating academic research to the verge of demonstrating practical technological enhancements in imaging and microscopy. Here, the aim is to give researchers from outside the quantum optical community, in particular those applying imaging technology, an overview of several promising quantum imaging approaches and evaluate both the quantum benefit and the prospects for practical usage in the near future. Several use case scenarios are discussed and a careful analysis of related technology requirements and necessary developments toward practical and commercial application is provided.

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Low‐coherent optical diffraction tomography by angle‐scanning illumination

Lee

Shin

Yaqoob

et al. 2019

Journal of Biophotonics

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We propose and experimentally demonstrate temporally low-coherent optical diffraction tomography (ODT) based on angle-scanning Mach–Zehnder interferometry. Using a digital micromirror device based on diffractive tilting, we successfully maintain full-field interference of incoherent light during every scan sequence. The ODT reconstruction principles for temporally incoherent illuminations are thoroughly reviewed and developed. Several limitations of incoherent illumination are also discussed, such as the nondispersive assumption, optical sectioning capacity, and illumination angle limitation. Using the proposed setup and reconstruction algorithms, we successfully demonstrate low-coherent ODT imaging of microspheres, human red blood cells, and eukaryotic cells.

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Ultraviolet Hyperspectral Interferometric Microscopy

Cited by 35 publications

References 19 publications

Deep UV dispersion and absorption spectroscopy of biomolecules

Deep UV dispersion and absorption spectroscopy of biomolecules

Perspectives for Applications of Quantum Imaging

Low‐coherent optical diffraction tomography by angle‐scanning illumination

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