Low‐coherent optical diffraction tomography by angle‐scanning illumination

Lee, KyeoReh; Shin, Seungwoo; Yaqoob, Zahid; So, Peter T. C.; Park, Yong Keun

doi:10.1002/jbio.201800289

Cited by 21 publications

(12 citation statements)

References 92 publications

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“…In 1969, Emil Wolf developed the theory, known as optical diffraction tomography (ODT), to reconstruct the object using the scattered fields [106]. ODT depends on either scanning the illumination angles or sample rotations to reconstruct the 3D object information [107][108][109]. The procedures of obtaining reconstructed tomograms with illumination rotation and cell rotation are illustrated in Fig.…”

Section: Tomographic Methods Based On Qpimentioning

confidence: 99%

Spatial light interference microscopy: principle and applications to biomedicine

2021

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In this paper, we review spatial light interference microscopy (SLIM), a commonpath, phase-shifting interferometer, built onto a phase-contrast microscope, with white-light illumination. As one of the most sensitive quantitative phase imaging (QPI) methods, SLIM allows for speckle-free phase reconstruction with sub-nanometer path-length stability. We first review image formation in QPI, scattering, holography, and microcopy. Then, we outline SLIM imaging from theory to instrumentation. Zernike's phase-contrast microscopy, phase retrieval in SLIM, and halo removal algorithms are discussed. Next, we discuss the requirements for operation, with a focus on software developed in-house for SLIM that high-throughput acquisition, whole slide scanning, mosaic tile registration, and imaging with a color camera. Lastly, we review the applications of SLIM in basic science and clinical studies. SLIM can study cell dynamics, cell growth and proliferation, cell migration, and mass transport, etc. In clinical settings, SLIM can assist with cancer studies, reproductive technology, and blood testing, etc. Finally, we review an emerging trend, where SLIM imaging in conjunction with artificial intelligence (AI) brings computational specificity and, in turn, offers new solutions to outstanding challenges in cell biology and pathology.

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Section: Tomographic Methods Based On Qpimentioning

confidence: 99%

Spatial light interference microscopy: principle and applications to biomedicine

2021

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“…In particular, phase-contrast tomography (PCT) probes the sample from different directions to achieve resolving capability along the optical axis and to measure the sample refractive index 3D distribution 16 – 22 . Multi-angle illumination can be realized by moving the light source with respect to the object 16 , 17 . Recently, in-flow cytotomography has been demonstrated by inducing controlled sample rotations in microfluidic streams, which is advantageous to maximize the throughput 22 .…”

Section: Introductionmentioning

confidence: 99%

Dehydration of plant cells shoves nuclei rotation allowing for 3D phase-contrast tomography

et al. 2021

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Single-cell phase-contrast tomography promises to become decisive for studying 3D intracellular structures in biology. It involves probing cells with light at wide angles, which unfortunately requires complex systems. Here we show an intriguing concept based on an inherent natural process for plants biology, i.e., dehydration, allowing us to easily obtain 3D-tomography of onion-epidermal cells’ nuclei. In fact, the loss of water reduces the turgor pressure and we recognize it induces significant rotation of cells’ nuclei. Thanks to the holographic focusing flexibility and an ad-hoc angles’ tracking algorithm, we combine different phase-contrast views of the nuclei to retrieve their 3D refractive index distribution. Nucleolus identification capability and a strategy for measuring morphology, dry mass, biovolume, and refractive index statistics are reported and discussed. This new concept could revolutionize the investigation in plant biology by enabling dynamic 3D quantitative and label-free analysis at sub-nuclear level using a conventional holographic setup.

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“…[26] On the other hand, red blood cells (RBCs) have also been interests for many researchers. [6,[27][28][29] Furthermore, the quantitative imaging capability of QPI and its synergistic applications in combination with artificial intelligence (AI) became one of the hot topics. [30][31][32][33][34][35][36] One of the important but not fully explored applications of QPI is the study of diatoms.…”

Section: Methods Detailsmentioning

confidence: 99%

Label-free imaging and analysis of subcellular parts of a living diatom cylindrotheca sp. using optical diffraction tomography

et al. 2020

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Optical diffraction tomography is an emerging label-free microscopic technique with its capability of label-free, quantitative, and rapid imaging of biological samples. In this work, we present the imaging and analysis of a living diatom Cylindrotheca sp. in seawater without using any pretreatment such as fluorescence staining. The 3D refractive index (RI) of a living diatom cell was measured, to which quantitative image analysis was perform to investigate subcellular parts of the diatom. Each part of the cell was well distinguished as RI values and distributions. From the analysis, RI values of frustules, protoplasm, vacuole, and chloroplast were estimated to be in the range of 1.

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Low‐coherent optical diffraction tomography by angle‐scanning illumination

Cited by 21 publications

References 92 publications

Spatial light interference microscopy: principle and applications to biomedicine

Spatial light interference microscopy: principle and applications to biomedicine

Dehydration of plant cells shoves nuclei rotation allowing for 3D phase-contrast tomography

Label-free imaging and analysis of subcellular parts of a living diatom cylindrotheca sp. using optical diffraction tomography

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