Two Examples of the -D-/-D- Genotype in an American Family

Abstract

Broom for confirmation. In view of the nearly equal titres for both L. icterohaemorrhagiae and L. canicola, a crossabsorption test was carried out. Dr. Broom's result is shown in Table II, clearly demonstrating L. canicola as the infecting organism. In addition, agglutination reactions against L. icterohaemorrhagiae and L. canicola were performed on blood samples from the patient's wife and eight children, and from three of his workmates. All results were negative. TABLE II.-Results of Cross-absorption Test Reciprocal of Titres L. icterohaemorrhagiae L. canzicola Unabsorbed .. ..

FURTHER INSTANCES OF POSSIBLE MUTATIONS AFFECTING THE Rh BLOOD GROUP SYSTEM

“…described the first example of blood failing S to react with any Rh test sera except those containing anti-D (antiRho) , similar observations have been reported by WALLER et al (1953( ), LEVINE et al (1954, BUCHANAN and MCINTYRE (1955), BUCHANAN (1956), andREAD et al (1961). Instances of blood failing to react with any anti-E (anti-rh") and anti-e (anti-hr") test sera have also been found (GUNSON andDONOHUE, 1957 andTATE et al, 1960) and, furthermore, in 1961, V o s et al described the blood of an Australian woman, which was proved by a number of test sera to lack all the known Rh antigens.…”

Determination of homozygote vs heterogote of blood group antigens with protein A.

“…It is now known that the Fc portion of the IgG molecule is the binding site and pure protein A is able to bind two molecules of IgG per molecule (Forsgren andSjoquist, 1966 : Kronvall andFrommel, 1970). however, the reaction is specific for subclass 1, 2 and 4 of human IgG (Hjelm et al, 1975: Sjoquist et al, 1972 et al, 1950 ;Waller et al, 1953). The traps-effect is similar in nature except that the competing Rh genes are located on the other autosome, traps to the gene being investigated.…”

A Serological Study of -D- Heterozygotes in the Same Family

“…Other families with -D-/-D-individuals have been described by Waller, Sanger and Bobbitt [18], Buchanan and McIntyre [2], Read, Brown, Linins, McIntyre and Moore [11], Yokoyama, Solomon, Kuniyuki and Stroop [20] and Ose and Bush [8]. These families were as certained because of transfusion problems with one of their -D-/-Dmembers.…”