The use of TDZ for the efficient in vitro regeneration and organogenesis of strawberry and blueberry cultivars

Cappelletti, Roberto; Sabbadini, Silvia; Mezzetti, Bruno

doi:10.1016/j.scienta.2016.05.016

Cited by 61 publications

(34 citation statements)

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“…micropropagation of different genotypes of Vaccinium species by several researchers (Cappelletti et al, 2016;Scalzo et al, 2016). For the shoot initiation, the aforesaid media with full strength were found to be appropriate and all gave successful results.…”

Section: Discussionmentioning

confidence: 99%

In vitro production protocol of Vaccinium uliginosum L. (bog bilberry)growing in the Turkish flora

Cüce¹,

Sökmen²

2017

Turk J Agric For

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Section: Discussionmentioning

confidence: 99%

In vitro production protocol of Vaccinium uliginosum L. (bog bilberry)growing in the Turkish flora

Cüce¹,

Sökmen²

2017

Turk J Agric For

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“…For V. corymbosum 'Oskar', V. angustifolium 'Emil' and 'Putte', and V. corymbosum × V. angustifolium 'Northblue' establishment, 2 mg L −1 (9.12 µM) ZEA was used [20]. In highbush blueberry 'Duke' propagation, ZEA at 2 mg L −1 (9.12 µM) was superior to 2iP or TDZ [21].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Establishment of ‘Delite’ Rabbiteye Blueberry Microshoots

Schuchovski

Biasi

2019

Horticulturae

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Micropropagation is an important technique for clonal mass propagation and a tool for in vitro studies. One of the first steps to overcome in this process is the establishment of new explants in vitro. ‘Delite’ rabbiteye blueberry was cultured in vitro with four cytokinins (zeatin (ZEA), 6-(γ-γ-dimethylallylamino)-purine (2iP), 6-benzylaminopurine (BAP), and kinetin (KIN)) at eight concentrations (0, 2.5, 5, 10, 20, 30, 40, and 50 µM). Additionally, nine combinations of nitrogen salts were tested, using Woody Plant Medium (WPM) and a modified WPM as the basic medium. ZEA and 2iP showed better responses, but ZEA was superior at lower (2.5 µM) concentrations (89.7% survival, 81.3% shoot formation, 1.3 shoots, 13.8 mm shoot length, 10.0 leaves). BAP and KIN showed very low responses. In the combinations of salts with modified WPM, no differences were observed. However, the original WPM with treatments of 0.5 × NH4NO3 and 1 × Ca(NO3)2, 0.5 × NH4NO3 and 0.5 × Ca(NO3)2, and the modified WPM alone showed the lowest rates of survival and shoot formation and the shortest shoot lengths. The highest shoot lengths were observed in treatments with the original WPM, 1.5 × NH4NO3 and 0.5 × Ca(NO3)2, and 1.5 × NH4NO3 and 1.5 × Ca(NO3)2. This initial study with ‘Delite’ can be the basis for further experiments with different combinations of salts, 2iP, and ZEA.

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“…It is a derivative of N , N ‐diphenylurea (DPU) and has adenine‐like cytokinin‐like activity. In agricultural production, it is primarily used to cultivate plant tissue (promote bud regeneration), promote cotton defoliation, increase disease resistance, and delay the decline of fruits, vegetables and fresh cut flowers . TDZ can promote fruit enlargement or increase crop yield.…”

Section: Introductionmentioning

confidence: 99%

Dissipation and the effects of thidiazuron on antioxidant enzyme activity and malondialdehyde content in strawberry

et al. 2019

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BACKGROUND: Increasing numbers of fruit swelling agents have been used to improve the fruit rate and production yield of strawberries in recent years. The abuse of fruit swelling agents could lead to an increase in the deformation rate and abnormal coloration of strawberry and a decrease in quality at harvest. Therefore, understanding the harmful effects of fruit swelling agents on strawberry will provide guidance for their reasonable use. RESULTS: The residual determination method for measuring thidiazuron (TDZ) in strawberry was developed and validated by liquid chromatography and tandem mass spectrometry (LC-MS/MS). The recoveries of TDZ in strawberry were 97.9-108.5% with relative standard deviations of 0.9% to 5.3%. The dissipation rates of TDZ were different in strawberries cultivated under field and indoor conditions due to the differences in temperature and humidity. The ascorbic acid content increased when TDZ was applied at 2 mg kg −1 . The SOD (superoxide dismutase), POD (peroxidase) and CAT (catalase) activities of strawberry tended to decrease and subsequently increase following the application of TDZ, and the opposite changes occurred on the malondialdehyde (MDA) content of TDZ-treated strawberry. CONCLUSIONS: The analytical method for measuring TDZ in strawberry that was developed was suitable for dissipation studies on this compound. Antioxidant enzyme activities and the MDA content of strawberry were altered, and some reverse effects, such as membrane damage, were inhibited when TDZ was applied. The data obtained in this study might provide suggestions to reduce the adverse effects of TDZ on strawberry and may help to guide the safe and proper use of TDZ in strawberry. Industry 1.7 μg kg −1 , respectively. After 5 days, the residue levels decreased to 1.0 μg kg −1 in both locations. All the dissipation data were substantially less than 50 μg kg −1 [maximum residue limit (MRL) set by China). The half-lives of TDZ in strawberry were between 5.2 J Sci Food Agric 2019; 99: 4331-4337

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The use of TDZ for the efficient in vitro regeneration and organogenesis of strawberry and blueberry cultivars

Cited by 61 publications

References 50 publications

In vitro production protocol of Vaccinium uliginosum L. (bog bilberry)growing in the Turkish flora

In vitro production protocol of Vaccinium uliginosum L. (bog bilberry)growing in the Turkish flora

In Vitro Establishment of ‘Delite’ Rabbiteye Blueberry Microshoots

Dissipation and the effects of thidiazuron on antioxidant enzyme activity and malondialdehyde content in strawberry

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