Dissipation and the effects of thidiazuron on antioxidant enzyme activity and malondialdehyde content in strawberry

Abstract

BACKGROUND: Increasing numbers of fruit swelling agents have been used to improve the fruit rate and production yield of strawberries in recent years. The abuse of fruit swelling agents could lead to an increase in the deformation rate and abnormal coloration of strawberry and a decrease in quality at harvest. Therefore, understanding the harmful effects of fruit swelling agents on strawberry will provide guidance for their reasonable use. RESULTS: The residual determination method for measuring thidiazuron (TDZ) in strawberry was developed and validated by liquid chromatography and tandem mass spectrometry (LC-MS/MS). The recoveries of TDZ in strawberry were 97.9-108.5% with relative standard deviations of 0.9% to 5.3%. The dissipation rates of TDZ were different in strawberries cultivated under field and indoor conditions due to the differences in temperature and humidity. The ascorbic acid content increased when TDZ was applied at 2 mg kg −1 . The SOD (superoxide dismutase), POD (peroxidase) and CAT (catalase) activities of strawberry tended to decrease and subsequently increase following the application of TDZ, and the opposite changes occurred on the malondialdehyde (MDA) content of TDZ-treated strawberry. CONCLUSIONS: The analytical method for measuring TDZ in strawberry that was developed was suitable for dissipation studies on this compound. Antioxidant enzyme activities and the MDA content of strawberry were altered, and some reverse effects, such as membrane damage, were inhibited when TDZ was applied. The data obtained in this study might provide suggestions to reduce the adverse effects of TDZ on strawberry and may help to guide the safe and proper use of TDZ in strawberry. Industry 1.7 μg kg −1 , respectively. After 5 days, the residue levels decreased to 1.0 μg kg −1 in both locations. All the dissipation data were substantially less than 50 μg kg −1 [maximum residue limit (MRL) set by China). The half-lives of TDZ in strawberry were between 5.2 J Sci Food Agric 2019; 99: 4331-4337

Enhancing fruit set, yield and quality of LeConte pear trees by preharvest foliar spray of some plant growth regulators

“…On the other hand, nitrophenolate sodium increased peel color of pear fruits. Such findings for GA, TDZ or nitrophenolate are in agreement with (Li et al, 2019;Yang et al, 2019;Attia, 2017;Hamed, 2018).…”

“…Moreover, TDZ application protects chlorophyll degradation, promotes cell division and elongation (Guo et al, 2011) which reflects on physical fruit quality. The positive role of TDZ on physical properties was previously reported (Carra et al, 2021;Li et al, 2019;Yang et al, 2019). Furthermore, preharvest application of nitrophenolate sodium enhanced physical characteristics of pear fruits.…”

Effect of Chitosan Coatings with Cinnamon Essential Oil on Postharvest Quality of Mangoes

“…The MDA content was measured as described by Yang et al [ 23 ]. One gram of mango tissue was homogenized with 5 mL of 10% trichloroacetic acid and then centrifuged for 20 min at 10,000× g .…”

Biochemical compounds and stress markers in lettuce upon exposure to pathogenic Botrytis cinerea and fungicides inhibiting oxidative phosphorylation

“…Our findings show, however, that higher activity of antioxidant enzymes is not constant during the dissipation of fungicides. Yang et al ( 2019 ) demonstrated increased catalase activity during the dissipation of thidiazuronon in strawberries, whereas increased activity of peroxidase under biotic stress conditions may be the result of cell wall lignification (Debona et al 2012 ). In contrast to increased POD activity after the use of imidacloprid (Sharma et al 2017 ), the present study shows a moderate defence role of POD from the very beginning of biotic ( B. cinerea ) and abiotic stress (fungicides used to inhibit oxidative phosphorylation).…”

“…Accordingly, they are not the first line of antioxidant defence. Stajner et al (2003) determined a lower SOD activity in lettuce treated with alachlor and metalachlor, whereas Yang et al (2019) could observe a relatively high SOD activity during the dissipation of thidiazuron. This shows that the effects of pesticides on antioxidant enzymes depend on an active ingredient's mode of action, plant species, and type of plant organs.…”