The Effects of Papain Enzyme Supplement in Feed on Protein Digestibility, Growth and Survival Rate in Sangkuriang Catfish (Clarias sp)

Abstract

The study aimed to assess the effects of Papain Enzyme Supplement in Feed on Protein Digestibity, Growth and Survival Rate of Sangkuriang Catfish (Clarias sp). Fingerlings used in the study were acquired from the Center for Freshwater Hatchery and Aquaculture, Muntilan, Central Java, Indonesia. The fingerlings had average weight of 3.43±0.50 g/fish. Completely Randomized Design was used in the experiments with 5 (five) treatment and 3 (three) repetitions. The experimental feed contained 31% of protein and 252.06 Kcal/g of energy. Various doses of the addition of papain enzyme were incorporated into the feed, those doses were A (0 g/kg feed), B (2 g/kg feed), C (4 g/kg feed), D (6 g/kg feed) and E (8 g/kg feed). Parameters of digestibility of protein (ADCP), relative growth rate (RGR), efficiency of feed utilization (EFU), feed conversion ratio (FCR), protein efficiency ratio (PER), survival rate (SR) and water quality were evaluated. The papain enzyme supplement significantly (P<0.01) influenced on ADCp, RGR, EFU, FCR, and PER, otherwise SR of catfish was insignificantly affected by the papain enzyme supplement. The best treatment result was the addition of 6/kg feed papain enzyme (treatment D). The study concluded that the optimum dose of the papain enzyme supplement for ADCp, EFU, FCR, PER and RGR were 5.65, 5.62, 6.0, 5.66, 6.0 g/kg feed respectively. Water quality during study was still in favorable condition for nurturing Sangkuriang catfish (Clarias sp).

Effect of flour-based feed of Tenebrio molitor l. on fresh water lobster (Cherax quadricarinatus von Martens) seed growth

“…Theoretically, a fairly good FCR value ranges from 0.8-1.6 [13]. Compared with other research, the FCR obtained from C. quadricarinatus with the addition of papain enzyme in commercial feed was 1.76 [14]. This figure is lower than the FCR results of this study.…”

“…However, after the ANOVA test, there was no significant effect (p= 0.089). Based on other research, adding papain enzyme in commercial feed recorded the highest FUE value of 75.09% [14]. Other research with similar treatment recorded the highest FUE value of 26.11 (±1.24)% [2].…”

Effects of Yeast on the Growth Performance of Sangkuriang Catfish Fingerlings (Clarias gariepinus var. Sangkuriang)

“…Sangkuriang) is highly related to feeding availability. Intensive aquaculture feed is the most significant share of cost production, around 50-60% (Rachmawati & Samidjan, 2018). Due to the inefficiency of feed to support growth, the enrichment of the commercial feed with yeast (Saccharomyces cerevisiae) is urgently needed.…”

Intake and digestibility of two diets for nine‐banded armadillos (Dasypus novemcinctus: Linnaeus, 1758) kept under human care

“…Papain is a rich antioxidant, anti-inflammatory, antibacterial, anthelmintic, antipyretic, and immunostimulant with hepatoprotective properties that help to improve cholesterol levels (Cherno et al, 2018;Oloruntola et al, 2020). Studies in aquatic animals demonstrate that including papain improves WG, performance, and diet digestibility (Rachmawati, Prihanto, Setyobudi et al, 2018;Rachmawati & Samidjan, 2018). Oloruntola et al (2020) observed that diets with papaya for broilers promoted the maintenance of normal intestinal microbiota, reduced serum cholesterol and alanine transaminase levels, and increased antioxidant enzyme effects, thereby improving animal performance and health status.…”