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The study aimed to assess the effects of Papain Enzyme Supplement in Feed on Protein Digestibity, Growth and Survival Rate of  Sangkuriang Catfish (Clarias sp).  Fingerlings used in the study were acquired from the Center for Freshwater Hatchery and Aquaculture, Muntilan, Central Java, Indonesia. The fingerlings had average weight of 3.43±0.50 g/fish. Completely Randomized Design was used in the experiments with 5 (five) treatment and 3 (three) repetitions. The experimental feed contained 31% of protein and 252.06 Kcal/g of energy. Various doses of the addition of papain enzyme were incorporated into the feed, those doses were A (0 g/kg feed), B (2 g/kg feed), C (4 g/kg feed), D (6 g/kg feed) and E (8 g/kg feed).  Parameters of digestibility of protein (ADCP), relative growth rate (RGR), efficiency of feed utilization (EFU), feed conversion ratio (FCR), protein efficiency ratio (PER), survival rate (SR) and water quality were evaluated.  The papain enzyme supplement significantly (P<0.01) influenced on ADCp, RGR, EFU, FCR, and PER, otherwise SR of catfish was insignificantly affected by the papain enzyme supplement. The best treatment result was the addition of 6/kg feed papain enzyme (treatment D). The study concluded that the optimum dose of the papain enzyme supplement for ADCp, EFU, FCR, PER and RGR were 5.65, 5.62, 6.0, 5.66, 6.0  g/kg feed respectively. Water quality during study was still in favorable condition for nurturing Sangkuriang catfish (Clarias sp).

A new culture system is introduced to maximize diet usage for growth of Catfish (Pangasius hypothalamus). The new culture system is by incorporating Saccharomyces cerevisiae into the feed. It aims to increase feed efficiency; in turn it increases the growth of fish. The objective of the research is to know the effects of S. cereviceae incorporated diet on growth performance, apparent digestibility coefficient of protein and survival rate of catfish (P. hypothalamus). The average wight of fish used in the study were 4.02 ± 0.22 g. The study used experimental method with completely random design, with 4 (four) treatments and each treatment had 4 (four) replications. The treatments were 4 different dosages of S. cerevisiae addition in the diet, i.e; 0 g per kg feed (A), 0.5 g per kg feed (B), 1 g per kg feed (C) and 2 g per kg feed (D). The results show that the incorporated of S. cerevisiae in feed has significant effect (P<0.05) on the specific growth rate (SGR), apparent digestibility coefficient of protein (ADC P) and survival rate (SR) of the catfish (P. hypothalamus) fingerlings. The optimum dosages of S. cerevisiae for SGR, ADC P and SR of the Catfish (P. hypothalamus) fingerlings ranged from 1 to 1.08 g/kg feed.

This study aims to examine the effect of phytase enzyme in the diet on digestibility of growth of white shrimp (Litopenaeus vannamei) raised in intensive system culture. Research on the suplementation of phytase enzymes in L. vannamei diet is very important to hydrolyze phytic acid in vegetable ingredients as a source of diet protein so as to increase the efficiency of diet utilization to support growth of vaname shrimp. The shrimp used in the research had average weight of 1,19±0,06 g/shrimp.  The diet contained 38% of protein and 3200 Kcal DE/kg with various doses of phytase enzyme suplementation, namely: A (0 U/kg diet), B (500 U/kg diet), C (1000 U/kg diet) and D (1500 U/kg diet).  Observed parameters were digestibility of phosphore (ADCF), disgetibility of protein (ADCP), efficiency of diet utilitzation (EFU), protein efficiency ratio (PER), diet convesion ratio (FCR) relative growth rate (RGR), and survival rate (SR).  The suplementation of phytase enzyme in the diet significantly influenced (P<0.01) on ADCF, ADCP, EFU, PER, FCR, RGR; however, it did not significantly influence on SR of white shrimp.  The best treatment result was the suplementation of 1000 U/kg diet phytase enzyme (treatment C). The optimum dose of phytase enzyme in the diet was 867, 920, 923, 915, 1000 and 1000 U/kg of diet respectively for ADCF, ADCP, EFU, PER, FCR, RGR. Based on those result the suplementation of phytase enzyme can increase the growth of white shrimp.

The success of the intensive culture of Sangkuriang catfish (Clarias gariepinus var. Sangkuriang) highly depends on the availability of feed. The feed is the most significant share of cost production hamper (50-60%). Therefore, it is suggested that feed utilization is inefficient. One of the solutions is to enrich the commercial feed with yeast (Saccharomyces cerevisiae). The research aimed to identify the impacts of yeast (S. cerevisiae) enhanced feed on feed efficiency, growth, and survival rate of Sangkuriang catfish fingerlings. The treatments research was yeast (S. cerevisiae) enrichment in the commercial feed at the various dosages: 0 %/kg feed (A), 3 %/kg feed (B), 6 %/kg feed (C), 9 %/kg feed (D), and 12 %/kg feed (E). The yeast (S. cerevisiae) enrichment in the commercial feed increased feed efficiency and survival rate of Sangkuriang catfish fingerlings. The optimum dosage of S. cerevisiae for apparent digestibility coefficients for protein (ADCp), efficiency feed utilization (EFU), protein efficiency ratio (PER), and relative growth rate (RGR) ranged from 6.10% to 6.51%/kg feed.

Pakan alami belum dapat digantikan oleh pakan buatan dalam kegiatan pembenihan. Thalassiosira sp. merupakan salah satu pakan alami sebagai pakan larva udang. Salah satu faktor yang mempengaruhi pertumbuhan pakan alami fitoplankton adalah mikronutrien. Mikronutrien tidak dapat dimanfaatkan oleh fitoplankton apabila tidak didukung adanya chelator. Chelator berfungsi untuk mensintesa mikronutrien di dalam media kultur agar bisa dimanfaatkan untuk proses metabolisme sel mikroalga. Tujuan dari penelitian ini adalah untuk mengkaji pengaruh rasio chelator dan metal pada media walne terhadap pola pertumbuhan dan kandungan protein Thalassiosira sp. Metode penelitian eksperimental menggunakan Rancangan Acak Lengkap (RAL) dengan 5 perlakuan 4 ulangan yaitu perlakuan A (tanpa chelator dan metal), perlakuan B (rasio chelator dan metal = 1:1), perlakuan C (rasio chelator dan metal = 2:1), perlakuan D (rasio chelator dan metal = 3:1) dan perlakuan E (rasio chelator dan metal = 4:1). Hasil penelitian menunjukkan bahwa rasio chelator dan metal berpengaruh nyata (P<0,05) terhadap nilai kepadatan akhir dan tidak berpengaruh nyata (P>0,05) terhadap nilai waktu lag phase, konstanta pertumbuhan spesifik (SGR) dan puncak populasi. Nilai kepadatan akhir tertinggi pada perlakuan C dengan nilai 50.000 sel/ml. Nilai waktu lag phase, konstanta pertumbuhan spesifik (SGR) dan puncak populasi berturut-turut berkisar antara -2,68 hingga (-2,73) hari, 0,354 hingga 0,362 dan 115.883 hingga 122.500 sel/ml. Kandungan protein tertinggi pada perlakuan C sebesar 52,37%. Perlakuan rasio chelator dan metal 2:1 berpengaruh terhadap pola pertumbuhan dan kandungan nutrisi Thalassiosira sp.Kata kunci: Chelator, metal, pola pertumbuhan, Thalassiosira sp.

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The objectives to study the effect of Biodiversity of Phytoplankton on the productivity in Polyculture Milkfish and White shrimp vanname Pond Culture waters, Kandang Panjang Pekalongan Region, Centre of Java. Methodology The sampling have tried station T1,T2,T3,T4 were in pond culture of polyculture waters to identify a phyto planton in polyculture pond Kandang Panjang waters. The sampling was carryout between Pebruary to Mei 2018. The result study showed that the value of the diversity indices in T1 treatment obtained the value of diversity indices (H’ = 1.093), T2 obtained diversity values (H’ = 1,072), T3 found (H’ = 1,804) and T4 showed (H’ = 1,907). The similliarity indexs are T1 (values, E = 0.765), values (E = 0.606), T3 ((E = 0.785) and T4 (E = 0.895).The dominance indices values phyto plankton showed T1 (value D = 0.725), T2 (D = 0.606,), T3(D = 00.595) and T4 (D = 00.578).The diversity indices showed higher vlues at T4 showed variety of diversity indices (H’ = 1,907).sampling site as compared to other three (T1,T2, and T3).During experiment showed the water quality of pond culture polyculture milkfish and white shrimp vanname was in viable condition.