The chemiluminescence immunoassay for aflatoxin B1 based on functionalized magnetic nanoparticles with two strategies of antigen probe immobilization

Abstract

A rapid and sensitive chemiluminescence immunoassay (CLIA) based on magnetic nanoparticles (MNPs) was developed to detect aflatoxin B1 (AFB1), which is a potent carcinogen in nature. We prepared monodisperse MNPs (300 nm in diameter) according to the solvothermal synthesis reaction and the MNPs were coated with silica by the Stöber method. Triethox was used as a one-step carboxylation reagent, and 3-aminopropyltriethoxysilane (APTES) an amination reagent, to modify the MNPs. We prepared two types of solid phase antigens using the above synthesized functionalized MNPs coupled with the later prepared AFB1-oxime active ester and the purchased BSA-AFB1 respectively. 2',6'-dimethylcarbonylphenyl-10-sulfopropylacridinium-9-carboxylate 4'-N-hydroxysuccinimide (4'-NHS) ester (NSP-DMAE-NHS), as a novel luminescent reagent, was used to label anti-AFB1 antibodies. The two CLIA calibration curves based on the two types of solid phase antigens were obtained and compared. The acquired limit of detection (LOD) was about 0.001 ng/mL for the two functionalized MNPs-based immunoassays, and the half maximal inhibitory concentration (IC ) was 0.51 ng/mL for the MNPs-AFB1-based method and 0.72 ng/mL for the MNPs-BSA-AFB1-based method.

Development and evaluation of the magnetic particle-based chemiluminescence immunoassay for rapid and quantitative detection of Aflatoxin B1 in foodstuff

“…Finally, the product was dried in a vacuum at 50°C. The AFB1-ALP was synthesized by the activated ester method (Yao, Wang, & Fang, 2017). Briefly, the AFB1 hapten was dissolved in 1 mL DMF, followed by adding 4 mg DCC and 4 mg NHS.…”

Time-Resolved Fluorescence Immunochromatography Assay (TRFICA) for Aflatoxin: Aiming at Increasing Strip Method Sensitivity

“…However, more and more producers want to use on-site assay methods for monitoring and ensuring their products' quality. Immunoassays have been developed especially for this aim (Kolosova et al, 2006;Tang et al, 2010;Masinde et al, 2013;Lin et al, 2017;Yao et al, 2017;Pan et al, 2018;Wang et al, 2018;Bhardwaj et al, 2019). Among the developed immunoassays, membrane-based strip assay for aflatoxin determination becomes more and more popular, although it was found to have lower sensitivity than ELISA (Wang et al, 2011;Anfossi et al, 2013).…”

Development of a Magnetic Nanoparticles-Based Screen-Printed Electrodes (MNPs-SPEs) Biosensor for the Quantification of Ochratoxin A in Cereal and Feed Samples

“…In recent years, magnetic nanoparticles (MNPs), owing to their uniform diameter particles that can be homogenously distributed in colloidal suspension, have been used widely in biomedical and food-safety assays [ 18 , 19 , 20 ]. When the magnetic nanoparticles (MNPs) are used for the preparation of immunomagnetic beads, a covalent bond binds the nanoparticle with the antibody to form a complex.…”