Development and evaluation of the magnetic particle-based chemiluminescence immunoassay for rapid and quantitative detection of Aflatoxin B1 in foodstuff

Abstract

Herein, a simple and cost-effective chemiluminescence immunoassay combined with the magnetic particles (MPCLIA) is presented for the rapid detection and analysis of Aflatoxin B1, which is a potent carcinogen found in the environment. Several physicochemical factors that potentially influence the assay performance of the proposed method were investigated in detail, including concentrations of FITC-labelled antibody, AFB1-alkaline phosphatase and magnetic particles concentration. Under the optimized experiment condition, the method showed: (i) a low limit detection of 0.05 ng/mL; (ii) satisfactory recoveries in real grain and oil samples ranging from 78% to 109%, which was well within the requirement of residue analysis, and (iii) excellent sensitivity, wide dynamic range and easy to separate in the washing process, which was superior to enzyme-linked immunosorbent assay (ELISA) method. In addition, the device used is of low cost. Moreover, the MPCLIA results for the real samples were in good agreement with that obtained by the high-performance liquid chromatography. These results suggest that the proposed MPCLIA method has potential application for screening other toxic mycotoxins occur in the food industry or other applied fields.

Recent Progress on Techniques in the Detection of Aflatoxin B1 in Edible Oil: A Mini Review

“…Recently, different nanomaterials, such as carbon and metal, have been used to modify the active surfaces of macroelectrodes and microelectrodes to design electrochemical biosensors for the detection of AFB 1 [ 193 , 194 , 195 ]. This is because new biosensors directly use nanomaterials or other materials containing nanoparticles that show significant characteristics, such as high sensitivity and specificity for detecting targets, reliability, and consistency of products [ 181 , 196 , 197 ]. Nanomaterials significantly increase the effective surface area of biosensors and further improve the analytical performance [ 60 , 194 ].…”

Rapid determination of aflatoxin B1 by an automated immunomagnetic bead purification sample pretreatment method combined with high‐performance liquid chromatography

“…The production of the anti‐AFB1 antibody immobilized onto immunomagnetic beads (AFB1‐IMBs) was performed according to the previous study with few modifications [32]. Typically, 20 mg/mL magnetic beads were rinsed with MES buffer (0.1 M, pH 6.0) for three times.…”

In-Line Technologies for the Analysis of Important Milk Parameters during the Milking Process: A Review

“…On the other hand, there are recent publications aimed at automated systems for the detection of aflatoxins. For example, Rhemrev et al dealt with the development of a reusable immunoaffinity cartridge where the antibody was coupled to a pressure-resistant polymer, making the cartridge usable in a commercially available automated system coupling the cleanup cartridge to the liquid chromatography system for analysis with fluorescence detection [85]. On the other hand, this study did not deal with the in-line detection of aflatoxins in milk, but in several plant products.…”