Recent Progress on Techniques in the Detection of Aflatoxin B1 in Edible Oil: A Mini Review

Yin, Shipeng; Niu, Liqiong; Liu, Yuanfa

doi:10.3390/molecules27196141

Cited by 16 publications

(5 citation statements)

References 266 publications

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“…Long-term exposure to aflatoxins can also result in chronic health effects, such as liver damage, for both cows and humans [8]. In humans, the breakdown of aflatoxins can contribute to the development of chronic diseases or severe cancers, leading to increased morbidity and mortality rates [9].…”

Section: Introductionmentioning

confidence: 99%

Aflatoxins Contamination in Animal Feeds and Fresh Milk in Kondoa District, Tanzania

Robert,

Nyamete,

Kabululu

et al. 2023

EJNFS

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The occurrence of aflatoxins in animal feeds and milk is common around the World as most livestock keepers are not aware of aflatoxins. Aims: This study was conducted to assess storage and feeding practices, and assessment of aflatoxin B1 and M1 contamination in animal feeds and fresh milk samples in Kondoa. Study Design: The study involved a survey using semi-structured questionnaires and Laboratory analysis of aflatoxin B1 and aflatoxin M1 in animal feeds and fresh milk samples collected from among smallholder dairy farmers. Methodology: Purposive selection of 120 respondents among smallholder dairy farmers in six wards purposively selected due to having larger number of dairy cattle was conducted. Likewise, 20 samples of animal feeds and 25 composite milk samples from among 120 interviewed farmers were analysed for aflatoxin B1 and M1 using High-Performance Liquid Chromatography (HPLC). Results: The results indicates that, aflatoxin B1 in feeds was 55%, with mean concentration of 8.6 ±5.9 μg/kg ranging from the lowest limit of detection (LOD) to 21.1 μg/kg with 5% samples above the maximum level stipulated by the Tanzania Bureau of Standards (TBS) and European Union (EU) of 20 μg/kg. Likewise, about 44% of the milk samples revealed to contain traces of aflatoxin M1 with a mean amount of 0.07 ±0.08 μg/L, ranging from the limit of detection (<LOD) to 0.23 μg/L, and 12% exceeding the TBS and EU standards of 0.05 μg/kg. Conclusion: There is need to scale up aflatoxin awareness campaigns and regular monitoring throughout the food system in Tanzania. GRAPHICAL ABSTRACT:

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Section: Introductionmentioning

confidence: 99%

Aflatoxins Contamination in Animal Feeds and Fresh Milk in Kondoa District, Tanzania

Robert,

Nyamete,

Kabululu

et al. 2023

EJNFS

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“…Antibodies are widely used in immunosensors because they can detect a variety of protein analytes with high sensitivity and selectivity. However, antibodies have some disadvantages, such as high synthesis cost, difficulty in production, low stability, and the possibility of aggregation, degradation, and denaturation when immobilized on the electrode [203].…”

Section: Impedimetric Detection Of Proteins Using Antibodiesmentioning

confidence: 99%

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Sitkov,

Ryabko,

Moshnikov

et al. 2024

Micromachines

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Impedimetric biosensors represent a powerful and promising tool for studying and monitoring biological processes associated with proteins and can contribute to the development of new approaches in the diagnosis and treatment of diseases. The basic principles, analytical methods, and applications of hybrid impedimetric biosensors for express protein detection in biological fluids are described. The advantages of this type of biosensors, such as simplicity and speed of operation, sensitivity and selectivity of analysis, cost-effectiveness, and an ability to be integrated into hybrid microfluidic systems, are demonstrated. Current challenges and development prospects in this area are analyzed. They include (a) the selection of materials for electrodes and formation of nanostructures on their surface; (b) the development of efficient methods for biorecognition elements’ deposition on the electrodes’ surface, providing the specificity and sensitivity of biosensing; (c) the reducing of nonspecific binding and interference, which could affect specificity; (d) adapting biosensors to real samples and conditions of operation; (e) expanding the range of detected proteins; and, finally, (f) the development of biosensor integration into large microanalytical system technologies. This review could be useful for researchers working in the field of impedimetric biosensors for protein detection, as well as for those interested in the application of this type of biosensor in biomedical diagnostics.

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“…Aflatoxin is the most harmful to human beings in food-borne mycotoxins, which can enter the human body through milk, oil crops and so on. Due to the potential for degradation of aflatoxins and the interaction of the resulting toxic compounds with food components, chronic disease or severe cancer may result, increasing morbidity and mortality rate [18]. Therefore, it is necessary to detect aflatoxin in food.…”

Section: Application Of Genetic Engineering In Detection Of Aflatoxinmentioning

confidence: 99%

Progress in the Application of Genetic Engineering in Life Detection

Wang¹

2023

HSET

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Since December 2019, the outbreak of SARS-CoV-2 has become a major public health challenge, which has a significant impact on the life, health and production of people around the world. Aflatoxin and kanamycin, which can enter the human body through food, and fluoride, which is widely present in the daily environment, are strong carcinogens. Therefore, the detection with high sensitivity, low detection limit and simple operation is needed for those pathogenic bacteria and compounds that endanger human health in life. However, the traditional laboratory operation has some defects, such as the need for expensive instruments, cumbersome operation and a certain demand for professionals. The rapid development of genetic engineering provides an excellent opportunity for the innovation of detection methods. In this paper, the contribution of genetic engineering in detection is briefly reviewed.

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Recent Progress on Techniques in the Detection of Aflatoxin B1 in Edible Oil: A Mini Review

Cited by 16 publications

References 266 publications

Aflatoxins Contamination in Animal Feeds and Fresh Milk in Kondoa District, Tanzania

Aflatoxins Contamination in Animal Feeds and Fresh Milk in Kondoa District, Tanzania

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Progress in the Application of Genetic Engineering in Life Detection

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