Development of a Magnetic Nanoparticles-Based Screen-Printed Electrodes (MNPs-SPEs) Biosensor for the Quantification of Ochratoxin A in Cereal and Feed Samples

Zhang, Xian; Wang, Zuohuan; Xie, Hui; Sun, Renjie; Cao, Tong; Paudyal, Narayan; Fang, Weihuan; Song, Houhui

doi:10.3390/toxins10080317

Cited by 23 publications

(14 citation statements)

References 44 publications

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“…Highly sensitive assays for the detection of AFB 1 , OTA, ZEN, or FB 1 have been established by other researchers [ 39 , 40 , 41 , 42 , 43 , 44 ], but for the detection of co-existing mycotoxins, multiple quantitative test assays, such as the microarray immunoassay described herein, will be more efficient at satisfying the need of detection. The assay we devised can detect four different mycotoxins in one single run, which is a pivotal advantage of this new method.…”

Section: Discussionmentioning

confidence: 99%

Antibody Microarray Immunoassay for Simultaneous Quantification of Multiple Mycotoxins in Corn Samples

Zhang

Wang

Fang

et al. 2018

Toxins

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We developed and tested a prototype of an antibody microarray immunoassay for simultaneous quantitative detection of four typical mycotoxins (aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1) in corn samples. The test kit consisted of a nitrocellulose membrane layered with immobilized monoclonal antibodies against mycotoxins. During the assay, the mycotoxin-protein conjugates were biotinylated. The signal detection was enhanced by a combination of the biotin-streptavidin system and enhanced chemiluminescence (ECL). This improved the sensitivity of the assay. Under the optimized conditions, four calibration curves with goodness of fit (R2 > 0.98) were plotted. The results showed that the detection limits for aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1 were 0.21, 0.19, 0.09, and 0.24 ng/mL, with detection ranges of 0.47–55.69, 0.48–127.11, 0.22–31.36, and 0.56–92.57 ng/mL, respectively. The limit of detection (LOD) of this antibody microarray for aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1 in corn was 5.25, 4.75, 2.25, and 6 μg/kg, respectively. The recovery rates from the spiked samples were between 79.2% and 113.4%, with coefficient of variation <10%. The results of the analysis of commercial samples for mycotoxins using this new assay and the liquid chromatography-tandem mass spectrometry (LC-MS/MS) were comparable and in good agreement. This assay could also be modified for the simultaneous detection of other multiple mycotoxins, as well as low-weight analytes, hazardous to human health.

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Section: Discussionmentioning

confidence: 99%

Antibody Microarray Immunoassay for Simultaneous Quantification of Multiple Mycotoxins in Corn Samples

Zhang

Wang

Fang

et al. 2018

Toxins

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“…Apart from the popular optical and electrochemical sensors, other interesting options based on m-NPs have been applied for food analysis. The main interest of these alternative sensors lies in the use of a different transduction principles (i.e., quartz crystal microbalance (QCM) [80,81], magnetic relaxation switching (MRS) [82][83][84], photoelectrochemistry [85,86]) or/and their particular arrangement (i.e., portable and low-cost sensors based on screen-printed electrode (ScPE) [73,85,[87][88][89][90][91][92], lateral flow strip (LFS) [93][94][95][96], microfluidic platforms [86,[97][98][99], and smartphone analysis [95,100,101]). Some selected examples of these kinds of devices are included in Table 6 [80,81,85,86,94,95,98,100,101].…”

Section: Other Types Of Sensorsmentioning

confidence: 99%

“…However, due to their particular arrangement and interesting advantages, a separate description is included here. Among the different strategies, the use of ScPE in combination with m-NPs has been the most popular for the analysis of food [73,83,[87][88][89][90][91][92]. ScPEs are planar systems with the three electrodes of the electrochemical cell arranged on a plastic, ceramic, textile or paper substrate.…”

Section: Other Types Of Sensorsmentioning

confidence: 99%

“…The minimal sample consumption of these devices, together with other advantages such as a mass-scale production, simplicity, selectivity, disposable use and flexibility, justifies their popularity [104]. In combination with m-NPs, ScPE has been used for the analysis of allergens [73], β-agonists [87], mycotoxins [92], bacteria [88], pesticides [89,90], polybrominated diphenyl ethers [91], and biogenic amines in seafood [73], meat [87,91], cereals [92], beverages [88,89], vegetables [90], milk [91], fish [91] and eggs [91]. For this purpose, functionalised m-NPs are coated with a suitable substrate and trapped at the ScPE surface with a magnet at the backside.…”

Section: Other Types Of Sensorsmentioning

confidence: 99%

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Recent Applications of Magnetic Nanoparticles in Food Analysis

et al. 2020

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Nanotechnology has become a topic of interest due to the outstanding advantages that the use of nanomaterials offers in many fields. Among them, magnetic nanoparticles (m-NPs) have been one of the most widely applied in recent years. In addition to the unique features of nanomaterials in general, which exclusively appear at nanoscale, these present magnetic or paramagnetic properties that result of great interest in many applications. In particular, in the area of food analysis the use of these nanomaterials has undergone a considerable increase since they can be easily separated from the matrix in sorbent-based extractions, providing a considerable simplification of the procedures. This allows reducing cost and giving fast responses, which is essential in the food trade to guarantee consumer safety. These materials can also be easily tunable, providing higher selectivity. Moreover, their particular electrical, thermal and optical characteristics allow enhancing sensor signals, increasing the sensitivity of the approaches based on this type of device. The aim of this review article is to summarise the most remarkable applications of m-NPs in food analysis in the last five years (2016–2020) showing a general view of the use of such materials in the field.

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“…S. aureus can adapt to grow in various foods and cause food poisoning by secreting enterotoxins [1,2]. Staphylococcal Enterotoxins (SEs) are proteins with 220 240 amino acids and have similar molecular weights (25)(26)(27)(28)(29)(30). sive equipment, and trained staff make them unsuitable.…”

Section: Introductionmentioning

confidence: 99%

Immunofluorescence Assay Using Monoclonal and Polyclonal Antibodies for Detection of Staphylococcal Enterotoxins A in Milk

Godjevargova¹,

Becheva²,

Ivanov³

et al. 2019

TOBIOTJ

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Objectives:Staphylococcus aureus is a Gram-positive microorganism. S. aureus can grow in various foods and cause food poisoning by secreting enterotoxins. The most common enterotoxins involved in food poisoning are staphylococcal enterotoxin A and staphylococcal enterotoxin B, but Staphylococcal Enterotoxin A (SEA) is predominant. The main types of food contaminated with SEs are meat and meat products, poultry and eggs, milk and dairy products. The aim of this study was to develop a rapid and sensitive fluorescence immunoassay for detection of staphylococcal enterotoxin A in milk. Methods:Monoclonal and polyclonal antibodies for SEA were produced and characterized. Competitive fluorescence immunoassay based on Magnetic Nanoparticles (MNPs) was performed and optimized. MNPs were used as a solid carrier of the antibodies. The first step of the assay was immunoreaction between the immobilized antibody onto MNPs and SEA in milk sample. Then the fluorescein-SEA conjugate was added to the sample. Thus, competitive immunoreaction between MNP-mAb/MNP-pAb with SEA and SEA-FITC was performed. These immuno-complexes were separated by a magnetic separator and the obtained supernatants were analyzed. The fluorescent signal from the excess of conjugated SEA was proportional to the SEA contained in the milk. The assay duration was only 30 min. Results:The fluorescence immunoassays performed with polyclonal antibody had linear ranges from 5 pg/mL to 100 ng/mL SEA in a buffer, and from 50 pg/mL to 50 ng/mL SEA in spiked milk samples. While the same assays performed with monoclonal antibody had linear ranges from 1 pg/mL to 20 ng/mL SEA in buffer, and from 10 pg/mL to 10 ng/mL SEA in spiked milk samples. The detection limits of the developed immunoassays performed in milk were: 48 pg/mL with polyclonal antibody and 9 pg/mL with monoclonal antibody. Conclusion:A rapid and sensitive fluorescence immunoassay based on magnetic nanoparticles with a polyclonal and monoclonal antibody for determination of staphylococcal enterotoxin A in milk was developed.

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Development of a Magnetic Nanoparticles-Based Screen-Printed Electrodes (MNPs-SPEs) Biosensor for the Quantification of Ochratoxin A in Cereal and Feed Samples

Cited by 23 publications

References 44 publications

Antibody Microarray Immunoassay for Simultaneous Quantification of Multiple Mycotoxins in Corn Samples

Antibody Microarray Immunoassay for Simultaneous Quantification of Multiple Mycotoxins in Corn Samples

Recent Applications of Magnetic Nanoparticles in Food Analysis

Immunofluorescence Assay Using Monoclonal and Polyclonal Antibodies for Detection of Staphylococcal Enterotoxins A in Milk

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