The antibacterial activities of bacteriocin Pediococcus acidilactici of breast milk isolate to against methicillin-resistant Staphylococcus aureus

Abstract

Resistance has occurred if an antibiotic cannot kill pathogenic bacteria therefore an antibiotic therapeutic effect cannot be achieved. Broad and free use of antibiotics effected in health problems, one of the health problems is bacteria are experienced the resistance such as Methicillin-Resistant Staphylococcus aureus (MRSA). Therefore an antibiotic alternative is needed from natural ingredients have the potential as anti-MRSA such as bacteriocin. This research was conducted to determine the antibacterial activity of bacteriocin from Pediococcus acidilactici of breast milk isolate (ASI) against MRSA. Bacteriocin isolation was carried out by growing of P.acidilactici on the media of Rogosa Sharpe (MRS) broth. Antibacterial activity test was carried out by diffusion method are to see the inhibition zone and dilution to determine the value of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The test results shown that bacteriocin P.acidilactici strains A11 and C12 strains had antibacterial activity against MRSA. The A11 strain inhibition zone is 15 mm, MIC and MBC values are 25%. While the C12 strain inhibition zone produced 18 mm, the value of MIC and MBC was 12.5%.

Bioprospecting of bacterial symbionts of sponge Spongia officinalis from Savu Sea, Indonesia for antibacterial potential against multidrug-resistant bacteria

“…Natural anti-MDR bacterial agents can be obtained from fruits (Prastiyanto et al 2020d), seeds (Prastiyanto et al 2020a;Prastiyanto 2021), Latex (Prastiyanto et al 2020c) lactic acid bacteria (Lestari et al 2019), mushroom (Prastiyanto et al 2020b(Prastiyanto et al , 2016, and bacterial isolates from marine organisms (Al-dhabi et al 2020) . In recent years, studies of marine bioactive compounds have yielded many drug candidates (Webster and Taylor 2012).…”

Antituberculosis agents of Lactobacillus plantarum and Pediococcus acidilactici Lactic Acid Bacteria in Breast milk isolates against Mycobacterium tuberculosis

“…L. plantarum and P. Acicilactici bacteria isolates were obtained from breast milk 14 . The isolates were then rejuvenated in the medium of MRS agar with 1% CaCO 3 and incubated at 35±2ºC for 2-3 days under anaerobic conditions.…”

Seeds extract of three Artocarpus species: Their in-vitro antibacterial activities against multidrug-resistant (MDR) Escherichia coli isolates from urinary tract infections (UTIs)

“…Antibacterial agents derived from biological sources include mushrooms (Prastiyanto et al 2016;2020b), lactic acid bacteria (Lestari et al 2019), latex (Prastiyanto et al 2020c), fruits (Prastiyanto et al 2020d), and seeds (Prastiyanto et al 2020a). Many studies on antimicrobial agents report the importance of plants as alternative antimicrobial agents (Prastiyanto et al 2021a;Prastiyanto et al 2021b).…”