This research studied the characteristics of bioplastic from large-leafed mangrove (Bruguiera gymnorrizha) starch. This research was arranged used Randomized Block Design (RBD) model, with different starch concentration (0.5%, 1%, 1.5% and 2%) as treatment. The parameters observed were mechanical properties (tensile strength and percent elongation), thickness, water uptake and biodegradation test. The result showed that the starch concentration was not significant, (P>0.05) affected tensile strength and water resistance. Different between treatments was observed as for elongation, thickness and biodegradation test significant (P<0.05). The results obtained from the bioplastic research of large-leafed mangrove starch for tensile strength ranged from 24.59 MPa – 32.91 MPa, percent elongation 2.93% – 4.88%, thickness 0.05 mm – 0,11 mm, water resistance 108.06% – 111.09% and biodegradation test with percent weight loss 17.91% – 54.40% with the highest degradation rate 18.13 – 3.62 mg /15 days burial. The best treatment was obtained by using 1,5% starch, 4 g chitosan and 15% glycerol or equal to starch : chitosan 1.5 g : 4 g and 0.9 mL glycerol.

Kualitas bekasam dapat ditingkatkan dengan menggunakan starter bakteri asam laktat (BAL) untuk mengontrol proses fermentasi. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan starter Lactobacillus acidophilus pada proses fermentasi bekasam terhadap karakteristik mikrobiologi, kimia dan sensoris, serta mengkaji sifat fungsional (kandungan lovastatin) dari bekasam ikan seluang (Rasbora argyrotaenia) yang telah diberi perlakuan penambahan starter L. acidophilus. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) dengan empat taraf perlakuan (0, 10^5, 10^7, 10^9 CFU/mL). Masing-masing perlakuan dilakukan pengulangan sebanyak 3 kali, dengan ulangan sebagai kelompok. Parameter yang diamati meliputi analisis total BAL, kimia, dan sensoris mutu hedonik. Hasil penelitian menunjukan bahwa perbedaan konsentrasi penambahan starter L. acidophilus dalam pembuatan bekasam ikan seluang berpengaruh nyata terhadap kadar protein, N-amino dan lovastatin bekasam. Starter L. acidophillus bisa ditambahkan dalam pembuatan bekasam sampai dengan konsentrasi 10^7 CFU/mL untuk meningkatkan kandungan lovastatin.

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Resistance has occurred if an antibiotic cannot kill pathogenic bacteria therefore an antibiotic therapeutic effect cannot be achieved. Broad and free use of antibiotics effected in health problems, one of the health problems is bacteria are experienced the resistance such as Methicillin-Resistant Staphylococcus aureus (MRSA). Therefore an antibiotic alternative is needed from natural ingredients have the potential as anti-MRSA such as bacteriocin. This research was conducted to determine the antibacterial activity of bacteriocin from Pediococcus acidilactici of breast milk isolate (ASI) against MRSA. Bacteriocin isolation was carried out by growing of P.acidilactici on the media of Rogosa Sharpe (MRS) broth. Antibacterial activity test was carried out by diffusion method are to see the inhibition zone and dilution to determine the value of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The test results shown that bacteriocin P.acidilactici strains A11 and C12 strains had antibacterial activity against MRSA. The A11 strain inhibition zone is 15 mm, MIC and MBC values are 25%. While the C12 strain inhibition zone produced 18 mm, the value of MIC and MBC was 12.5%.

The purpose of the research was to know physic and chemical characteristics of siamese gouramy fish sauce using three different fermentation processes. This research method used a faktorial randomized block design (FRBD) with two treatment factor and done with three replications. The factor treatment were consists of a type of fermentation (spontaneous, pedioccus halophilus, enzyme papain) and fermentation time (30. 60. 90 day). This research shows that the different of fermentation type and fermentation time had significant effect on yield, pH, ash content, protein content, tvb and interaction between fermentation type and fermentation time had significant effect on chrome. The best product is papain enzyme addition and long fermentation during 90 days that produce the product had yield (58.8%), pH (6.04), ash content (23.82%), protein content (2.46 mg/mL), tvb (94.58 mg/100mg), viskositas (0.65 dPas), lightness (26.6), chrome (2.17), and hue (41)

ABSTRAKPenelitian ini bertujuan untuk menentukan waktu optimum produksi enzim protease dan amilase isolat bakteri asal terasi ikan teri Stolephorus sp. Penelitian ini dilakukan dalam dua tahap yaitu pengukuran pertumbuhan isolat bakteri dan penentuan waktu optimum produksi enzim protease dan amilase. Pengujian yang dilakukan meliputi uji aktivitas enzim protease dan amilase dan  kadar protein dari tiap-tiap enzim tersebut. Data hasil pengujian dianalisis secara deskriptif. Ada 4 isolat bakteri, 2 isolat merupakan bakteri penghasil protease yaitu isolat P2 dan P4, dan 2 isolat yang merupakan bakteri penghasil amilase yaitu A2 dan A4. Aktivitas protease optimum terjadi pada jam ke-36 untuk isolat P2 sebesar 0,073 U/mL dengan aktivitas spesifik sebesar 1,632 U/mg dan isolat P4 yaitu 0,057 U/mL dengan aktivitas spesifik 4,91U/mg. Aktivitas amilase optimum terjadi pada jam ke-36 untuk isolat A2 sebesar 0,360 U/mL dengan aktivitas spesifik sebesar 7,73 U/mg dan aktivitas amilase optimum pada isolat A4 sebesar 0,239 U/mL dengan aktivitas spesifik 5,24 U/mg. ABSTRACTThe purpose of this research was to know the optimization of incubation time in the production of protease and amylase by bacterial isolates of anchovy Stolephorus sp. paste origin. This research was conducted in two stages, namely the growth of bacterial isolates measurement and determination the optimum time production of the enzyme protease and amylase. Testing conducted include the test proteases and amylase enzyme activity and protein levels of each of these enzymes. The data will be analyzed test results are descriptive. There are 4 bacterial isolates, where 2 isolates, which is a protease-producing bacteria isolates that is P2 and P4, and 2 isolates, which is the amylase-producing bacteria that is A2 and A4. The activity of the protease optimum occurs at to-36 hours to isolate P2 of 0.073 U/mL with a specific activity of 1.632 U/mg and isolates P4 that is 0.057 U/mL with a specific activity of 4.91 U/mg. Whereas amylase activity, optimum occurs at to-36ohours for A2 isolates of 0.360 U/mL with a specific activity of 7.73 U/mg and activity of amylase optimum on A4 isolates of 0.239 U/mL with a specific activity of  5.224 U/mg.

Water lettuce (Pistia stratiotes) is macrophyte plant that grows in nearly all tropical fresh waterways and floats on the water surface. The purpose of this study was to quantify the phytochemical constituents and vitamins which contribute to antioxidant activity of this plant. N-hexane, ethyl acetate, and methanol extracts of water lettuce leaf were screened for the presence of phenols and tannins following Folin-Ciocalteu method. The vitamin C and E content of fresh leaves were determined using HPLC. The results show that the phenolic content of methanol extract was the highest with the value of 109.05 ppm while the ethyl acetate extract exhibits the highest tannins (138.86 ppm). Total contents of 0.52 mg and 3.36 mg were respectively obtained for vitamin C and vitamin E in 100 g of the sample. These results indicate the natural presence of antioxidants in the extract.