Bioprospecting of bacterial symbionts of sponge Spongia officinalis from Savu Sea, Indonesia for antibacterial potential against multidrug-resistant bacteria

Abstract

Abstract. Prastiyanto ME, Kartika AI, Darmawati S, Radjasa OK. 2022. Bioprospecting of bacterial symbionts of sponge Spongia officinalis from Savu Sea, East Nusa Tenggara, Indonesia for antibacterial potential against multidrug-resistant bacteria. Biodiversitas 23: 1118-1124. Marine sponge Spongia sp. has been reported to have potential as an antibacterial agent. However, less information on the potential of bacterial symbionts of Spongia sp. as an antibacterial agent has been documented. The present investigation involves isolating bacterial symbionts of sponge Spongia officinalis and the characterization of antibacterial potential against multidrug-resistant bacteria isolated from clinical specimens. Spongia officinalis was collected from Savu Sea, East Nusa Tenggara, Indonesia and its symbionts were isolated with Zobell marine agar media. The overlay method was used to screen the antibacterial activity against selected six MDR bacteria. Antibacterial activity was determined by measuring the diameter of the inhibition zone. Identification of active bacterial symbionts was carried out based on the 16S rRNA gene sequencing. The results revealed that four out of 10 symbionts showed antibacterial activity against MDR bacteria with an inhibition index ranging from 4.8 to 12.6 mm. Prastiyanto-1A and Prastiyanto-1E isolates demonstrated antibacterial activity against ESBL- Escherichia coli and ESBL + CRE- Klebsiella pneumoniae subsp. pneumoniae, Pratiyanto-2A isolate showed antibacterial activity against MRSA, while Prastiyanto-4A isolates showed antibacterial activity against CRPA. The selected four isolates were identified as Bacillus subtilis, Bacillus mojavensis and Bacillus simplex using 16S rRNA gene sequencing and BLASTn analysis. These results provide information about the potential of bacterial symbionts of S. officinalis as natural antibacterial sources against MDR bacteria.

A Mini Review on Analysis of Potential Antibacterial Activity of Symbiotic Bacteria from Indonesian Freshwater Sponge: An Unexplored and A Hidden Potency

“…Bacillus is one of the most versatile bacteria in biosynthesis since it can produce a broad variety of bioactive compounds such as non-ribosomal peptide synthase (NRPS) and Polyketide synthase (PKS) (Prastiyanto et al 2022). Furthermore, bacillus could also produce compounds with anti-bacterial properties.…”

“…Besides providing energy for a certain host sponge, e.g., photosynthetic cyanobacteria, other microorganisms contribute to sponges' defense mechanism through the production of a bioactive compound or secondary metabolites (Unson et al 1994;Schmidt et al 2000), which has been researched intensively for a decade as antibacterial potency of bacterial symbionts from sponges. In Indonesia, several bioprospecting studies have been conducted on screening these bacteria symbionts in sponges for antibacterial potency, particularly from marine sponges e.g., Xestospongia testudinaria from Papua (Cita et al 2017), Aaptos suberitoides, Agelas nakamurai and other eight marine species from Sulawesi (Riyanti et al 2020), Spongia officinalis from Nusa Tenggara (Prastiyanto et al 2022) and Jaspis sp. from Enggano (Sipriyadi et al 2022).…”

Isolation and Molecular Identification of Endophytic Fungi Associated with Brown Algae for Inhibiting Escherichia coli ESBL

“…is isolate was identi ed and its susceptibility pattern was obtained using the Vitek®MS (bioMerieux) test (Prastiyanto et al, 2022). e test bacteria were rst rejuvenated in nutrient agar (NA) slant (Merck, UK) and incubated for 24 hours at 37 C. Subsequently, 100 µL of bacterial culture which had been incubated at 37 C for 24 hours was added to 20 mL of new NA media.…”

Antibacteria Activity Peel and Seed Extracts of Rambutan (Nephelium lappaceum L.) Against MDR Bacteria Causing Urinary Tract Infections

“…Therefore, numerous options are required to treat cases of resistant bacteria, such as the use of natural ingredients. Antibacterial agents can be obtained from various sources, for example, Latex (Prastiyanto et al, 2020b), Mushrooms (Prastiyanto et al, 2020a), marine bacteria (Prastiyanto et al, 2022c(Prastiyanto et al, , 2022b(Prastiyanto et al, , 2023, and plant (Prastiyanto et al, 2021a(Prastiyanto et al, , 2021b(Prastiyanto et al, , 2022aPrastiyanto, 2021). One of the natural ingredients that can be used as antibacterial agents is the rambutan (Nephelium lappaceum L.).…”