Seeds extract of three Artocarpus species: Their in-vitro antibacterial activities against multidrug-resistant (MDR) Escherichia coli isolates from urinary tract infections (UTIs)

Abstract

Abstract. Prastiyanto ME. 2021. Seeds extract of three Artocarpus species: Their in-vitro antibacterial activities against multidrug-resistant (MDR) Escherichia coli isolates from urinary tract infections (UTIs). Biodiversitas 22: 4362-4368. Multidrug-resistant (MDR)-E. coli is a major cause and has become a very serious problem in urinary tract infections (UTIs). As a result, it requires an antibacterial agent derived from biological materials. It has been reported that the seeds of three species of Artocarpus (A. heterophyllous, A. champeden, and A. camansi) have antibacterial properties against Methicillin-Resistant Staphylococcus aureus (MRSA). However, there are three other Artocarpus species in Indonesia, i.e., keledang (A. lanceipolius), tarra (A. elasticus), and terap (A. Odoratissimus) whose antibacterial property has not been investigated. To minimize the research gap, this study aims to determine the antibacterial activity of seed extracts of A. lanceipolius, A. elasticus, and A. odoratissimus against MDR-E. coli isolates of UTIs. Antibacterial activity was evaluated using the agar well diffusion assay. The microdilution method was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values. The results revealed that the seed extracts of A. lanceipolius, A. elasticus, and A. odoratissimus have the potential as antibacterial agents against MDR-E. coli isolate of UTIs. A. elasticus seed extract shows the widest zone of inhibition in the range of 7.0-13.3 mm and the smallest MIC and MBC values ??of 6.25-12.5 mg/mL and 12.5-25 mg/mL, respectively. In conclusion, A. lanceipolius, A. elasticus, and A. odoratissimus seed extracts have the potential to be developed as antibacterial agents against UTI-causing MDR-E. coli. Further in vivo research and determining the mode of action of antibacterial activity are needed.

Bioprospecting of bacterial symbionts of sponge Spongia officinalis from Savu Sea, Indonesia for antibacterial potential against multidrug-resistant bacteria

“…Natural anti-MDR bacterial agents can be obtained from fruits (Prastiyanto et al 2020d), seeds (Prastiyanto et al 2020a;Prastiyanto 2021), Latex (Prastiyanto et al 2020c) lactic acid bacteria (Lestari et al 2019), mushroom (Prastiyanto et al 2020b(Prastiyanto et al , 2016, and bacterial isolates from marine organisms (Al-dhabi et al 2020) . In recent years, studies of marine bioactive compounds have yielded many drug candidates (Webster and Taylor 2012).…”

Antibacterial activity of seed kernel extracts of seven mangoes (Mangifera indica) cultivars native to Indonesia against MDR-Pseudomonas aeruginosa isolated from wounds

“…The colonies were non-lactose fermenters selected using positive catalase and oxidase testing. Vitek®MS was used for bacterial identification and susceptibility testing (Prastiyanto 2021). The following antibiotics used as positive controls were AMP (Ampicillin), TZP (Piperacillin-tazobactam), SAM (Ampicillin-sulbactam), CAZ (Ceftazidime), CFZ (Cefazolin), FEP (Cefepime), CRO (Ceftriaxone), ATM (Aztreonam), TGC (Tigecycline), MCA (Amikacin), CIP (Ciproflox), MEM (Meropenem), and GEN (Gentamicin).…”

“…Agar well diffusion assay was used to evaluate the antibacterial activity of mango seed extracts against MDR-P. aeruginosa (Prastiyanto 2021). MDR-P aeroginusa isolates from patient wounds were cultured on a blood agar plate and then incubated for 24 hours at 35 ± 2C.…”

“…Therefore, alternative antibacterial sources from natural ingredients are needed. Natural antibacterial sources comprise marine microorganisms (Prastiyanto et al 2022), lactic acid bacteria (Lestari et al 2019), mushrooms (Prastiyanto et al 2020a), fruit (Prasnto et al 2020c, seeds (Prastiyanto 2021), and latex (Prastiyanto et al 2020b). Plants are the most common and potential antibacterial agents from natural sources of antibacterial agents (Prastiyanto et al 2021), one of which is mango.…”

Antibacterial Activity of Ketapang (Terminalia cattapa L.) Leaf Extract Against Staphylococcus aureus and Pseudomonas aeruginosa Isolates of Diabetic Wounds

“…The antibacterial activity test using agar well diffusion assay [10] was conducted by 100 µL bacterial culture equal to the McFarland standard of 0.5 innoculated on the surface of Muller Hilton Agar (MHA/OXOID) media through the dense streak using the sterilized ocular inoculation. After set aside for 10 min, starting from the initial inoculation, then a well was made in each agar plate using the sterilized steel Cork Borer (diameter 0.6 cm).…”