Synergistic anticancer effects of phycocyanin and Citrullus colocynthis extract against WiDr, HCT-15 and HCT-116 colon cancer cell lines

Hamdan, Noor T.; Jwad, Balkeas Abd Ali Abd Aun; Jasim, Saade Abdalkareem

doi:10.1016/j.genrep.2020.100972

Cited by 14 publications

(8 citation statements)

References 16 publications

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“…Caspase-3 has attracted much attention for its role as the executor of cell apoptosis. The results of this study showed that the expression levels of Bax and caspase-3 increased while the expression level of Bcl-2 decreased [38].…”

Section: Discussionmentioning

confidence: 56%

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Anti-Cancer Properties of Coix Seed Oil against HT-29 Colon Cells through Regulation of the PI3K/AKT Signaling Pathway

Ding

et al. 2021

Foods

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This study aims to observe the effects of coix seed oil (CSO) on HT-29 cells and investigate its possible regulation mechanism of the PI3K/Akt signaling pathway. Fatty acid analysis showed that coix seed oil mainly contains oleic acid (50.54%), linoleic acid (33.76%), palmitic acid (11.74%), and stearic acid (2.45%). Fourier transform infrared results found that the fatty acid functional groups present in the oil matched well with the vegetable oil band. The results from CCK-8 assays showed that CSO dose-dependently and time-dependently inhibited the viability of HT-29 cells in vitro. CSO inhibited cell viability, with IC50 values of 5.30 mg/mL for HT-29 obtained after 24 h treatment. Morphological changes were observed by apoptotic body/cell nucleus DNA (Hoechst 33258) staining using inverted and fluorescence microscopy. Moreover, flow cytometry analysis was used to evaluate the cell cycle and cell apoptosis. It showed that CSO induced cell apoptosis and cycle arrest in the G2 phase. Quantitative real-time PCR and Western blotting revealed that CSO induced cell apoptosis by downregulating the PI3K/AKT signaling pathway. Additionally, CSO can cause apoptosis in cancer cells by activating caspase-3, up-regulating Bax, and down-regulating Bcl-2. In conclusion, the results revealed that CSO induced G2 arrest and apoptosis of HT-29 cells by regulating the PI3K/AKT signaling pathway.

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Section: Discussionmentioning

confidence: 56%

“…PI3K, Forward The data were analyzed using the 2 −∆∆Ct method. Using β-actin as a reference, the relative mRNA expression level in the sample was calculated, and the results were characterized by the ratio of gene expression to the blank [38].…”

Section: Real-time Pcr (Rt-pcr)mentioning

confidence: 99%

Anti-Cancer Properties of Coix Seed Oil against HT-29 Colon Cells through Regulation of the PI3K/AKT Signaling Pathway

Ding

et al. 2021

Foods

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“…Citrullus colocynthis has an anticancer effect, antioxidant and anti metastatic effect [44]. The expression of bax gene in Citrullus colocynthis and the combination of extract of phycocyanin has higher inhibitory effect on cancer cells [45] but in my research its was found that ethanolic leaf extract showed anicancerous effect on cancerous cell [46,47] and also has found that Citrullus lanatus showed inhibited growth on human leukemia cancer cells [48,49] but in my research we have used another herbal plants Citrullus colocynthis was taken has plant sampling and the study proves that Citrullus colocynthis also has anticancer effects. Through this methanolic extract of Artemisia absinthium it was found the anticancer effect on colorectal cancer HCT-116 cell line.…”

Section: Discussionmentioning

confidence: 99%

Induction of Bax and Activation of Caspases by Hydro Ethanolic Leaf Extract of Citrullus colocynthis (L)-mediated Apoptosis in Breast Cancer Cell Line (MCF-7)

Bharath

Priya

Jayaraman

et al. 2021

JPRI

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Cancer is the most deadly disease that causes death among the world. It is caused by genome and epigenome abnormalities. Herbal plants are used for treatment of cancer. Citrullus colocynthis is valuable medicinal plants used for treatment of cancer. The plant as antidiabetic, anticancer activity, and antioxidant activity.The aim of the study is to find induction of bax and activation of caspases by hydro ethanolic leaf extract of Citrullus colocynthis (L)-in Human breast cancer cells (MCF-7). Materials and methods, Human breast cancer cell was purchased from the national centre for cell sciences. Cell line and cell culture were performed and cell viability was done by MTT assay. Total RNA isolation, cDNA conversion and gene expression analysis by real time PCR. This study clearly indicates that plant extract has a significant role in modulation of intrinsic apoptotic signaling in human breast cancer cells which might be due to the presence of cucurbitacin present in Citrullus colocynthis. It is conducted that Citrullus colocynthis extract inhibits the growth of the human breast cancer cells (MCF-7) by regulating the expression of pro apoptotic (Bax) and caspases -3 genes. Hence Citrullus colocynthis may be served as a potential drug for treatment of human breast cancer cells.

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“…R-PE can inhibit the growth of subcutaneous transplanted tumors, repair damaged mucosa to protect the intestinal barrier, and regulate the immune function of mice ( Qi et al, 2019 ). Regarding PC, studies have indicated that this pigment can induce apoptosis, one of the important mechanisms in the inhibition of cancer cell proliferation, of multiple non-small cell lung cancer cells and colorectal cancer cells ( Hao et al, 2019 ; Hamdan et al, 2021 ).…”

Section: Phycobiliproteins Applicationsmentioning

confidence: 99%

Extraction and purification of phycobiliproteins from algae and their applications

et al. 2022

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Microalgae, macroalgae and cyanobacteria are photosynthetic microorganisms, prokaryotic or eukaryotic, living in saline or freshwater environments. These have been recognized as valuable carbon sources, able to be used for food, feed, chemicals, and biopharmaceuticals. From the range of valuable compounds produced by these cells, some of the most interesting are the pigments, including chlorophylls, carotenoids, and phycobiliproteins. Phycobiliproteins are photosynthetic light-harvesting and water-soluble proteins. In this work, the downstream processes being applied to recover fluorescent proteins from marine and freshwater biomass are reviewed. The various types of biomasses, namely macroalgae, microalgae, and cyanobacteria, are highlighted and the solvents and techniques applied in the extraction and purification of the fluorescent proteins, as well as their main applications while being fluorescent/luminescent are discussed. In the end, a critical perspective on how the phycobiliproteins business may benefit from the development of cost-effective downstream processes and their integration with the final application demands, namely regarding their stability, will be provided.

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Synergistic anticancer effects of phycocyanin and Citrullus colocynthis extract against WiDr, HCT-15 and HCT-116 colon cancer cell lines

Cited by 14 publications

References 16 publications

Anti-Cancer Properties of Coix Seed Oil against HT-29 Colon Cells through Regulation of the PI3K/AKT Signaling Pathway

Anti-Cancer Properties of Coix Seed Oil against HT-29 Colon Cells through Regulation of the PI3K/AKT Signaling Pathway

Induction of Bax and Activation of Caspases by Hydro Ethanolic Leaf Extract of Citrullus colocynthis (L)-mediated Apoptosis in Breast Cancer Cell Line (MCF-7)

Extraction and purification of phycobiliproteins from algae and their applications

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