Synchronized electromechanical integration recording of cardiomyocytes

Hu, Ning; Wang, Tianxing; Wan, Hao; Zhuang, Liujing; Kettenhofen, Ralf; Zhang, Xiaoyu; Zhang, Yu Shrike; Xu, Wei; Goßmann, Matthias; Bohlen, Heribert; Hou, Xu; Wang, Ping

doi:10.1016/j.bios.2018.06.017

Cited by 43 publications

(18 citation statements)

References 53 publications

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“…[15] Therefore, molecular imprinting has been applied, as an alternative, in selective adsorption of small biomolecules or biomacromolecules. [16][17][18] However, the current adsorption/purification methods are restricted to the capture of single target, while simultaneous capture of multiple molecules, as is often the case with actual blood sample, remains challenging. In addition, most of the bulk adsorbent materials face the dilemma of insufficient contact with the target molecules, as well as a lack of fluidic environment where applications for blood cleansing should take into account.…”

Section: Doi: 101002/adma202005394mentioning

confidence: 99%

Hierarchically Molecular Imprinted Porous Particles for Biomimetic Kidney Cleaning

et al. 2020

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Blood purification by adsorption of excessive biomolecules is vital for maintaining human health. Here, inspired by kidney self‐purification, which removes a number of biomolecules with different sizes simultaneously, hierarchical molecular‐imprinted inverse opal particles integrated with a herringbone microfluidic chip for efficient biomolecules cleaning are presented. The particle possesses combinative porous structure with both surface and interior imprints for the specific recognition of small molecules and biomacromolecules. Additionally, the presence of the herringbone mixer largely improve the adsorption efficiency due to enhanced mixing. Moreover, the inverse opal framework of the particles give rise to optical sensing ability for self‐reporting of the adsorption states. These features, together with its reusability, biosafety, and biocompatibility, make the platform highly promising for clinical blood purification and artificial kidney construction.

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Section: Doi: 101002/adma202005394mentioning

confidence: 99%

Hierarchically Molecular Imprinted Porous Particles for Biomimetic Kidney Cleaning

et al. 2020

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“…[210] In our previous work, we designed a dual functional sensor chip integrated with IDEs and microelectrodes to monitor the physical state and electrophysiological function of cardiomyocytes simultaneously, which can be applied in drug-induced cardiotoxicity studies [211][212][213] and marine toxins detection. [214] Furthermore, by increasing the sampling rate of IDEs, this microelectrode-IDEs biosensor enables the synchronized electromechanical integration recording of cardiomyocytes over a long time, which can be applied to drug-induced cardiotoxicity screening (Figure 9A) [215] and ion channel blockers recognition. [216] Also, new materials and fabrication methods are introduced into cardiomyocyte biosensing technologies.…”

Section: Future Perspectivesmentioning

confidence: 99%

“…Adapted with permission. [ 215 ] Copyright 2018, Elsevier. B) 3D multifunctional integumentary membranes (3D‐MIMs) for spatiotemporal measurement and stimulation across the entire epicardial surface and the representative optical and electrical signals acquired simultaneously from a Langendorff‐perfused rabbit heart.…”

Section: Future Perspectivesmentioning

confidence: 99%

Advances in Multidimensional Cardiac Biosensing Technologies: From Electrophysiology to Mechanical Motion and Contractile Force

Wei

Zhuang

et al. 2020

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Cardiovascular disease (CVD) is the number one killer in the world. According to the World Health Organization (WHO) estimation, about 17.9 million people died from CVD each year, accounting for 31% of all deaths worldwide. [1] Among these deaths, about 7.4 million died from coronary heart disease, while 6.7 million from a stroke. According to China cardiovascular disease report in 2018, [2] around 290 million people suffer from CVD, including 13 million strokes, 11 million coronary heart disease, 5 million pulmonary heart disease, 4.5 million heart failure, 2.5 million rheumatic heart disease, 2 million congenital heart disease, and 270 million hypertension. Cardiovascular disease was also the leading cause of all death in rural and urban areas, with 45.01% in rural areas and 42.61% in urban areas. According to heart disease and stroke statistics from the American Heart Association (AHA) in 2018, [3] around 92.1 million American adults have a certain form of cardiovascular disease or suffer from stroke sequelae. CVD killed 836546 Americans in 2015. Coronary heart disease was the leading cause (43.8%) of death in CVD, followed by stroke (16.8%), heart failure (9.0%), hypertension (9.4%), arterial disease (3.1%) and other cardiovascular diseases (17.9%). According to the European cardiovascular disease statistics in 2017, [4] around 3.9 million people died from CVD each year, which accounts for 45% of all deaths in Europe. Ischemic heart disease (IHD) and stroke are the main forms of CVD in Europe. Among all deaths in Europe each year, IHD is the leading single cause of mortality, responsible for 19% and 20% among men and women, while stroke is the second most common single cause of death, accounting for 9% in men and 13% in women. Therefore, the prevention and treatment of CVD have aroused extensive public concern worldwide. Moreover, cardiac toxicity induced by drugs has been the main cause of the withdrawal from the market and drugs attrition during the last decades. [5,6] On the one hand, drug-induced cardiotoxicity will also threaten people's health, even life. For example, acute arrhythmia can lead to sudden death, while Cardiovascular disease is currently a leading killer to human, while druginduced cardiotoxicity remains the main cause of the withdrawal and attrition of drugs. Taking clinical correlation and throughput into account, cardiomyocyte is perfect as in vitro cardiac model for heart disease modeling, drug discovery, and cardiotoxicity assessment by accurately measuring the physiological multiparameters of cardiomyocytes. Remarkably, cardiomyocytes present both electrophysiological and biomechanical characteristics due to the unique excitation-contraction coupling, which plays a significant role in studying the cardiomyocytes. This review mainly focuses on the recent advances of biosensing technologies for the 2D and 3D cardiac models with three special properties: electrophysiology, mechanical motion, and contractile force. These high-performance multidimensional cardiac models are popular and ef...

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“…To counteract these methodological limitations, electrochemical impedance spectroscopy (EIS) has been widely applied for noninvasive, real-time, high-throughput analysis of cell viability, proliferation, and cytotoxicity 13,14 . Based on the EIS technique, electric cell-substrate impedance sensing (ECIS) has been used for real-time and label-free monitoring of 2D cell behaviors, such as cell viability, proliferation, and apoptosis [15][16][17] . The impedance changes detected by ECIS are determined by the viability and number of cells attached to 2D planar interdigitated electrodes (IDEs) 18 .…”

Section: Introductionmentioning

confidence: 99%

3D microgroove electrical impedance sensing to examine 3D cell cultures for antineoplastic drug assessment

Pan

Jiang

et al. 2020

Microsyst Nanoeng

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In recent decades, three-dimensional (3D) cancer cell models have attracted increasing interest in the field of drug screening due to their significant advantages in more accurate simulations of heterogeneous tumor behavior in vivo compared to two-dimensional models. Furthermore, drug sensitivity testing based on 3D cancer cell models can provide more reliable in vivo efficacy prediction. The gold standard fluorescence staining is hard to achieve real-time and label-free viability monitoring in 3D cancer cell models. In this study, a microgroove impedance sensor (MGIS) was specially developed for the dynamic and noninvasive monitoring of 3D cell viability. 3D cancer cells were trapped in microgrooves with gold electrodes on opposite walls for in situ impedance measurement. The change in the number of live cells caused inversely proportional changes to the impedance magnitude of the entire cell/Matrigel construct and reflected the proliferation and apoptosis of the 3D cells. It was confirmed that the 3D cell viability detected by the MGIS was highly consistent with the standard live/dead staining by confocal microscope characterization. Furthermore, the accuracy of the MGIS was validated quantitatively using a 3D lung cancer model and sophisticated drug sensitivity testing. In addition, the parameters of the MGIS in the measurement experiments were optimized in detail using simulations and experimental validation. The results demonstrated that the MGIS coupled with 3D cell culture would be a promising platform to improve the efficiency and accuracy of cell-based anticancer drug screening in vitro.

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Synchronized electromechanical integration recording of cardiomyocytes

Cited by 43 publications

References 53 publications

Hierarchically Molecular Imprinted Porous Particles for Biomimetic Kidney Cleaning

Hierarchically Molecular Imprinted Porous Particles for Biomimetic Kidney Cleaning

Advances in Multidimensional Cardiac Biosensing Technologies: From Electrophysiology to Mechanical Motion and Contractile Force

3D microgroove electrical impedance sensing to examine 3D cell cultures for antineoplastic drug assessment

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