Studies on growth and cardenolide production of Digitalis lanata tissue cultures

Abstract

Digitalis lanata cell cultures grown as small undifferentiated aggregates in suspension culture can be redifferentiated into green embryos that produce cardenolides. The possibility of using a statistical (Box-Wilson) experimental design to study the effects of four different variables on growth, differentiation, and cardenolide production of D. lanata tissue cultures are investigated. The results of the analyses were processed by linear regression analysis. Mathematical models explaining the effects of the variables were developed. The concentration of maltose and the NO(3) (-)--NH(4) (+) ratio were found to be significant variables for both growth and cardenolide production. The size of the inoculum was also important.

Optimization of sucrose and inorganic nitrogen concentrations for somatic embryogenesis of birch (Betula pendula Roth.) callus cultures: A statistical approach

“…Systematic optimization of growth conditions is widely used with microbes and has also been used with plant cell cultures for optimization of Digitalis lanata culture media (Tuominen et al 1989). The aim of this work was to test systematic optimization as a means of determining the optimum sucrose and inorganic nitrogen concentrations for the somatic embryogenesis of birch.…”

Diseño de bioprocesos y bioproductos en ingeniería de células vegetales

“…El diseño del método ortogonal se usó para optimizar el crecimiento de Cassia didymobotrya, así como su producción de metabolitos secundarios (Botta et al, 1989). El método de respuesta superficial se adaptó para optimizar el crecimiento y producción de metabolitos secundarios de Digitalis lanata (Tuominen et al, 1989) y para la optimización de alcaloides indol producidos por Catharantus roseus (Schlatman et al, 1992). Los métodos de Plackett-Burman y el método de respuesta superficial se usan para optimizar el cultivo (glucosa y ácido acético-3-indol) y parámetros ambientales (tamaño del inoculo y pH) para el crecimiento de Podophyllum hexandrum y producción de podofilotoxina, de esta forma se incrementó la producción de este metabolito pasando de 4.6 mg/L a 13.8 mg/L en fermentadores mecánicamente agitados .…”

Cell and tissue cultures of Catharanthus roseus: A literature survey

“…Two different statistical experimental designs have been employed for medium optimisation with plant cell cultures, namely the orthogonal design method (Botta et al 1989) and the response surface method (Tuominen et al 1988). Statistical experimental design is a helpful tool for optimisation using a minimum of experiments.…”