Cell and tissue cultures of Catharanthus roseus: A literature survey

Abstract

The literature concerning the regulation and the biosynthesis of secondary metabolites in cell and tissue cultures of Catharanthus roseus is reviewed. The aim of this review is to summarise the progress achieved since the previous review of this subject from 1988 to December 1993. Several factors influencing the production of indole alkaloids are discussed. Special attention is given to large-scale cultivation methods. Some economic considerations on the production of ajmalicine are also discussed. ranth~ ro~eu~ cited in ~ C~c~ Abs~acts ~tween 1950 ~d 1993.most valuable components, the dimeric alkaloids vincristine and vinblastine, have so far only been detected in callus or organ cultures of C. roseus (Miura & Hirata 1982; Hoffmann et al. 1982;Miura et al. 1988).

Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Indole Alkaloids Isolated from <i>Catharanthus roseus </i> (L.) G. Don Cultivated Conventionally and Derived from In vitro Cultures

“…Vitamins and organic components had slight effect on the alkaloids production. Only in case of glucose positive effect on ajmalicine production in hairy-root culture was noted by Moreno et al (1995). On the other hand, Zhao et al (2001) showed that the addition of succinic acid, tryptamine and tryptophan to the culture medium caused significant growth of ajmalicine and catharanthine levels.…”

Application of carborundum abrasion for investigating the leaf epidermis: molecular cloning of Catharanthus roseus 16‐hydroxytabersonine‐16‐O‐methyltransferase

“…VBL and VCR are produced as a result of a condensation of the Iboga alkaloid catharanthine and the aspidosperma alkaloid vindoline. While catharanthine-accumulating cell suspension cultures have been successfully produced (Moreno et al, 1995), cultures that make vindoline have never been reported. The complex regulation of vindoline biosynthesis, including the requirement for light and the involvement of several cell types in its production (St Pierre et al, 1999), could not be reproduced in cell culture systems.…”

Zinc Finger Proteins Act as Transcriptional Repressors of Alkaloid Biosynthesis Genes in Catharanthus roseus

“…Terpenoid indole alkaloid biosynthesis in Catharanthus roseus is one of the best studied elicitorinduced secondary metabolic pathways. In suspension cells, the perception of yeast extract (YE) leads to the activation of terpenoid indole alkaloid biosynthesis (5). Two genes involved in terpenoid indole alkaloid biosynthesis, encoding strictosidine synthase (STR) and tryptophan decarboxylase (TDC), are coordinately regulated and their mRNAs accumulate transiently after YE treatment (6,7).…”