Optimization of sucrose and inorganic nitrogen concentrations for somatic embryogenesis of birch (Betula pendula Roth.) callus cultures: A statistical approach

Nuutila, Anna-Maria; Kurten, Ulrika; Kauppinen, Veli

doi:10.1007/bf00039733

Cited by 23 publications

(13 citation statements)

References 8 publications

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“…ABA is another hormone which plays a major role in the embryo, by preventing precocious germination (Kermode 1995) and promoting the accumulation of storage compounds (Cailloux et al 1996), and thus, embryo maturation (Black 1991). In somatic embryogenesis ABA reduces the frequency of embryo malformation (Etienne et al 1993) and the occurrence of secondary or repetitive embryogenesis (Nuutila et al 1991).…”

Section: Introductionmentioning

confidence: 99%

Dynamics of biochemical and morphophysiological changes during zygotic embryogenesis in Acca sellowiana (Berg.) Burr.

et al. 2009

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Acca sellowiana (Berg.) Burr. is a native Myrtaceae from southern Brazil and Uruguay, now the subject of a domestication and breeding program. Biotechnological tools have been used to assist in this program. The establishment of a reliable protocol of somatic embryogenesis has been pursued, with a view to capturing and fixing genetic gains. The rationale behind this work relies on the fact that deepening comprehension of the general metabolism of zygotic embryogenesis may certainly improve the protocol for somatic embryogenesis. Thus, in the present work we studied the accumulation of protein, total sugars, starch, amino acids, polyamines (PAs), IAA and ABA, in different stages of A. sellowiana zygotic embryogenesis. Starch is the predominant storage compound during zygotic embryo development. Increased synthesis of amino acids in the cotyledonary stage, mainly of asparagine, was observed throughout development. Total free PAs showed increased synthesis, whereas total conjugated PAs were mainly observed in the early developmental stages. IAA decreased and ABA increased with the progression from early to late embryogenesis. Besides providing basic information on the morphophysiological and biochemical changes of zygotic embryogenesis, the results here obtained may provide adequate strategies towards the modulation of somatic embryogenesis in this species as well as in other woody angiosperms.

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Section: Introductionmentioning

confidence: 99%

Dynamics of biochemical and morphophysiological changes during zygotic embryogenesis in Acca sellowiana (Berg.) Burr.

et al. 2009

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“…This medium is later referred to as N73 medium. It was originally designed for embryo production with the 6/3 cell line in callus culture (Kurtdn et al 1990;Nuutila et al 1991).…”

Section: E D I U Mmentioning

confidence: 99%

“…plantlets are commercially produced by micropropagation (T6rmiil/i 1990), but the use of plant cell cultures for production of somatic plant embryos is a promising alternative to the traditional plant propagation methods. Somatic embryogenesis of birch is possible in callus culture on agar plates (Kurt6n et al 1990;Nuutila et al 1991).…”

Section: Introductionmentioning

confidence: 99%

Nutrient uptake and growth of an embryogenic and a non-embryogenic cell line of birch (Betula pendula Roth.) in suspension culture

Nuutila

Kauppinen

1992

Plant Cell Tiss Organ Cult

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The nutrient uptake of an embryogenic and of a non-embryogenic cell line of birch (Betula pendula Roth.) during cell growth and embryo production was studied in suspension culture. The embryogenic and non-embryogenic cell suspensions grew differently in the same medium. The non-embryogenic cell line started to grow without any lag period after the inoculation. It rapidly hydrolyzed sucrose in the medium to glucose and fructose and consumed the glucose as carbon source. The concentration of fructose in the medium decreased only after the depletion of glucose. The embryogenic cell line also rapidly hydrolyzed the sucrose to glucose and fructose, but the monosaccharides were consumed only after the embryos started to germinate after three weeks of culture. Both monosaccharides were then taken up at the same rate.

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“…), and N7 basal medium was used as the medium for initiation (Simola 1985). Nuutila et al (1991) studied effect of different sugar and inorganic nitrogen concentrations in in vitro cultures of birch. Embryogenic callus was initiated on N7 medium, on which somatic embryos were obtained in a later stage (Simola 1985).…”

Section: Short Review Of Economically Important Coniferous and Decidumentioning

confidence: 99%

Somatic Embryogenesis in Forest Plants

Chorabik¹

2012

Embryogenesis

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Optimization of sucrose and inorganic nitrogen concentrations for somatic embryogenesis of birch (Betula pendula Roth.) callus cultures: A statistical approach

Cited by 23 publications

References 8 publications

Dynamics of biochemical and morphophysiological changes during zygotic embryogenesis in Acca sellowiana (Berg.) Burr.

Dynamics of biochemical and morphophysiological changes during zygotic embryogenesis in Acca sellowiana (Berg.) Burr.

Nutrient uptake and growth of an embryogenic and a non-embryogenic cell line of birch (Betula pendula Roth.) in suspension culture

Somatic Embryogenesis in Forest Plants

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