Nutrient uptake and growth of an embryogenic and a non-embryogenic cell line of birch (Betula pendula Roth.) in suspension culture

Nuutila, Anna-Maria; Kauppinen, V.

doi:10.1007/bf00039996

Cited by 11 publications

(4 citation statements)

References 13 publications

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“…Culture of somatic embryos of Populus (Michler & Bauer, 1991) on solid medium arrested their development at the torpedo stage and, since this effect was not observed in liquid medium, the authors claimed that nutrient availability was a possible reason for that result. Starvation also improved the embryogenic potential of birch cells in suspension culture (Nuutila & Kauppinen, 1992).…”

Section: Maintaining and Controlling Repetitive Embryogenesismentioning

confidence: 99%

Influence of external factors on secondary embryogenesis and germination in somatic embryos from leaves of Quercus suber

Fernández-Guijarro¹,

Celestino

Toribio

1995

Plant Cell Tiss Organ Cult

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Somatic embryogenesis was obtained in cultures of leaves from young seedlings of Quercus suber L. A twostage process, in which benzyladenine and naphthaleneacetic acid were added first at high and then at low concentrations, was required to initiate the process. Somatic embryos arose when the explants were subsequently placed on medium lacking plant growth regulators. The embryogenic lines remained productive, by means of secondary embryogenesis, on medium without growth regulators. However, this repetitive induction was influenced by the macronutrient composition of the culture medium. Both low total nitrogen content and high reduced nitrogen concentration decreased the percentage of somatic embryos that showed secondary embryogenesis. Our results suggest that alternate culture on medium that increases embryo proliferation and a low salt medium prohibiting embryo formation will partially synchronize embryo development. Chilling slightly reduced secondary embryogenesis but gave a modest increase in germination. Maturation under light followed by storage at 4 °C for at least 30 days gave the best results in switching embryos from an embryogenic pathway to a germinative one. Under these conditions 15% of embryos showed coordinated root and shoot growth and 35% formed either shoots or mostly roots. These percentages were higher than those of embryos matured in darkness. This result indicates that a specific treatment is required after maturation and before chilling to activate the switch from secondary embryo formation to germination.

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Section: Maintaining and Controlling Repetitive Embryogenesismentioning

confidence: 99%

Influence of external factors on secondary embryogenesis and germination in somatic embryos from leaves of Quercus suber

Fernández-Guijarro¹,

Celestino

Toribio

1995

Plant Cell Tiss Organ Cult

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“…During the bioreactor cultivations of lines V2 and C23, sucrose was hydrolyzed to glucose and fructose during the first and second week of cultivation, but the monomer sugars were not consumed until during the third and fourth week of cultivation. Nuutila and Kauppinen (21) reported similar results for embryogenic birch cells in suspension culture. Of the two nitrogen sources, ammonium was consumed first.…”

Section: Discussionmentioning

confidence: 59%

Heterologous Expression of Vitreoscilla Hemoglobin (VHb) and Cultivation Conditions Affect the Alkaloid Profile of Hyoscyamus muticus Hairy Roots

et al. 2006

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Fast-growing hairy root cultures of Hyoscyamus muticus induced by Agrobacterium rhizogenes offer a potential production system for tropane alkaloids. Oxygen deficiency has been shown to limit growth and biomass accumulation of hairy roots, whereas little experimental data is available on the effect of oxygen on alkaloid production. We have investigated the effect of Vitreoscilla hemoglobin (VHb) expression and cultivation conditions on the complete alkaloid profile of H. muticus hairy roots in shake flasks and in a laboratory scale bioreactor. We optimized the growth medium composition and studied the effects of sucrose, ammonium, nitrate, and phosphate on growth and alkaloid production. Maximum biomass accumulation was achieved with the highest and maximum hyoscyamine content with the lowest sucrose concentration. The optimum nitrate concentration for growth was higher for the VHb line than the control. Neither VHb expression nor aeration improved the hyoscyamine content significantly, thus suggesting that hyoscyamine biosynthesis is not limited by oxygen availability. Interestingly, the effect of VHb expression on the alkaloid profile was slightly different from that of aeration. VHb expression did not affect the concentrations of cuscohygrine, which was increased by aeration. Therefore, the effect of VHb is probably not related only to its ability to increase the intracellular effective oxygen concentration.

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“…These findings suggest that the accumulation of F in the culture medium of R. jasminoides is not linked to an inability to utilize this monosaccharide, but rather to the fact that G is a better substrate for cell respiration, as reported by Sagishima et al (1989) for cell suspension cultures of Catharanthus roseus. The preferential uptake of G has also been described for cell suspension cultures of carrot (Kanabus et al, 1986;Bender et al, 1987), soybean (Spilatro and Anderson, 1988), birch (Nuutila and Kauppinen, 1992), and bean (Botha and O_Kennedy, 1998) and has been attributed to a higher affinity of the sugar transport proteins for this monosaccharide when compared to F (Tubbe and Buckhout, 1992;Sherson et al, 2003). Therefore, the behavior of cell suspension cultures of R. jasminoides regarding sugar utilization seems to be typical for undifferentiated cell suspension cultures of dicotyledons.…”

Section: Resultsmentioning

confidence: 82%

Differential sugar uptake by cell suspension cultures of Rudgea jasminoides, a tropical woody Rubiaceae

Kretzschmar

Júnior

Braga

2007

In Vitro Cell.Dev.Biol.-Plant

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The primary utilization of carbohydrates by cell suspension cultures of Rudgea jasminoides, a native woody Rubiaceae from tropical forests, was investigated. Sucrose, glucose + fructose, glucose, or fructose were supplied as carbon sources. The growth curves of R. jasminoides cultured in glucose + fructose, glucose, or fructose showed similar patterns to that observed when sucrose was supplied to the cells, except that an increase in dry mass was observed at the beginning of the stationary growth phase in the media containing only one monosaccharide. The increase in hexose levels in the media during the early stages of the cultures indicated extracellular hydrolysis of sucrose, which was further supported by the increase in the activity of acid invertase bound to the cell wall. Glucose was preferentially taken up, whereas uptake of fructose was delayed until glucose was nearly depleted from the medium. Measurements of intracellular sucrose content and cytoplasmatic and vacuolar invertases indicate that the enzymatic activity seems to be correlated with a decrease in the hexose flux into the cells of R. jasminoides. Our results indicate that the behavior of cell suspension cultures of R. jasminoides regarding sugar utilization seems to be similar to other dicotyledonous undifferentiated cell suspension cultures.

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Nutrient uptake and growth of an embryogenic and a non-embryogenic cell line of birch (Betula pendula Roth.) in suspension culture

Cited by 11 publications

References 13 publications

Influence of external factors on secondary embryogenesis and germination in somatic embryos from leaves of Quercus suber

Influence of external factors on secondary embryogenesis and germination in somatic embryos from leaves of Quercus suber

Heterologous Expression of Vitreoscilla Hemoglobin (VHb) and Cultivation Conditions Affect the Alkaloid Profile of Hyoscyamus muticus Hairy Roots

Differential sugar uptake by cell suspension cultures of Rudgea jasminoides, a tropical woody Rubiaceae

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