Differential sugar uptake by cell suspension cultures of Rudgea jasminoides, a tropical woody Rubiaceae

Kretzschmar, Fernanda S.; Júnior, Clovis José Fernandes de Oliveira; Braga, Márcia R.

doi:10.1007/s11627-006-9001-x

Cited by 17 publications

(12 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, in the present study, glucose by itself was not promotory for bud-break and shoot length, whereas combined presence of glucose and fructose, derived through sucrose hydrolysis, was noted as being essential for the highest percentage of bud-break and shoot length. In the combined presence of glucose and fructose, cells exhibit a preference for glucose whereas fructose is utilized as a substrate only after glucose is depleted from the medium (Kretzschmar et al 2007). Thus, the presence of sucrose felicitated the constant availability of utilizable forms of carbohydrates in the medium, throughout the culture period.…”

Section: Discussionmentioning

confidence: 99%

Improved clonal propagation of Spilanthes acmella Murr. for production of scopoletin

Singh

Chaturvedi

2010

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

A reproducible protocol for clonal propagation of Spilanthes acmella has been established. Routinely, the cultures were established in spring (January-April) season because of the highest aseptic culture establishment and high frequency shoot proliferation. Incorporation of 5 lM N 6 -benzyladenine (BA) to Murashige and Skoog (MS) basal medium showed 100% bud-break and promoted multiple shoot proliferation in cultures. Interestingly, a higher concentration of BA (7-15 lM) promoted stunted shoots with pale leaves while a lower concentration (1-3 lM) resulted in shoots with long internodes and excessive adventitious root proliferation from all over their surface. For recurrent shoot multiplication, single node segments from in vitro-developed shoots were excised and cultured on MS ? BA (5 lM) medium where 20.3-fold shoot multiplication was achieved every 5 weeks. Finally, these shoots were successfully rooted on half-strength MS medium (major salts reduced to half-strength) with 50 g l -1 sucrose, with a frequency of 100%. Transplantation survival of micropropagated plants was 88.9%. Additionally, accumulation of scopoletin, a phytoalexin, was revealed for the first time in the uninfected leaves of Spilanthes. Further, the quantitative estimation by HPLC with a fluorescence detector showed that the amounts of scopoletin content (0.10 lg g -1 DW) in the leaves of micropropagated plants are comparable to those of field-grown mother plants. The study thus signifies the effectiveness of in vitro methodology for true-to-type plant regeneration of Spilanthes and their later utility for biosynthesis and constant production of scopoletin throughout the year.

show abstract

Section: Discussionmentioning

confidence: 99%

Improved clonal propagation of Spilanthes acmella Murr. for production of scopoletin

Singh

Chaturvedi

2010

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…Differential affinity for glucose and fructose has been reported for many plant cell cultures (Sherson et al 2003;Kretzschmar et al 2007 and references therein). These monosaccharides represent the principal source of carbon and energy in most cells, and a significantly higher affinity of the membrane transporters for glucose is almost the rule.…”

Section: Discussionmentioning

confidence: 99%

“…However, some plants have preferential requirements for glucose or sucrose (Kato et al 2007). Sucrose administered externally to plant cell suspensions is usually rapidly hydrolyzed to glucose and fructose by cell wall invertases (b-fructofuranosidases; EC 3.2.1.26) (Goetz and Roitsch 2000;Kretzschmar et al 2007). The role of invertases in sucrose uptake is regarded as prominent in sink tissues in which there is no symplastic continuity between cells (i.e.…”

Section: Introductionmentioning

confidence: 99%

“…The role of invertases in sucrose uptake is regarded as prominent in sink tissues in which there is no symplastic continuity between cells (i.e. maternal and embryo tissues of developing seeds) (Jain et al 2008;Privat et al 2008), and in the case of cultured cells (cell suspensions and calluses) where cell wall connections are very loose (Koch 2004;Kretzschmar et al 2007). In the case of cultured tissues, however, invertases may be secreted into the medium also (Shin et al 2003) and a novel isoform of acid invertase, which is not bound to the cell wall, has been characterized (Kim et al 2000).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sugar metabolism ofVetiveria zizanioidescells cultured with Amberlite XAD-4. Uptake of radiolabelled sugars, cell wall invertase activity and gene expression

Mucciarelli¹,

Bertea

Camusso³

2009

Journal of Plant Interactions

View full text Add to dashboard Cite

Activated and non-activated Amberlite XAD-4 was added to the cell cultures of vetiver (Vetiveria zizanioides Stapf.) in order to study its effects as abiotic growth promoter. Amberlite XAD-4 increased vetiver cell viability, especially when used in the activated form. Results from labelling experiments in cell suspensions showed that XAD-4 prompted a significant decrease of sucrose Km, accompanied by an increased Vmax. Glucose uptake from the medium was almost doubled, as demonstrated by the increase of the radiolabelled fraction (25-fold the controls) in vetiver cells cultured with XAD-4. Affinity for fructose uptake by cells was not altered by the use of activated XAD-4, even though its uptake and Vmax increased. These results were further supported by a significant 7.5-fold increase in cell wall invertases (CWI) activity in XAD-4 elicited cells. Vetiver CWI gene expression was characterized by transcript RT-PCR analysis. The identity of the transcripts was confirmed by sequencing analysis. These results indicated the use of XAD-4 as an effective tool for vetiver cell biomass optimization.

show abstract

“…Since then, suspension-cultured cells of R. jasminoides have been used as a model system to study the biological activity of cell wall fragments (Oliveira Júnior et al 2006), to evaluate the production of constitutive fungitoxic compounds and anthraquinones (Oliveira et al , 2009 and to understand the differential uptake of sugars by cells of a tree Rubiaceae species (Kretzschmar et al 2007). The homogeneous culture of R. jasminoides consists of predominantly undifferentiated cells with mainly primary cell walls (Stella and Braga 2002).…”

Section: Introductionmentioning

confidence: 99%

Cell wall polysaccharides from cell suspension cultures of the Atlantic Forest tree Rudgea jasminoides (Rubiaceae)

Júnior

Cavalari

Carpita

et al. 2010

Trees

Self Cite

View full text Add to dashboard Cite

Rudgea jasminoides (Rubiaceae) is a tropical tree species native of the Atlantic Forest in the south of Brazil. Previous studies with leaf cell walls of R. jasminoides showed a different proportion of cross-linked glycans compared to what is usually reported for eudicots. However, due to the difficulties of working with whole plant organs, cell suspensions of R. jasminoides, consisting of predominantly undifferentiated cells with mainly primary cell walls, were used to examine cell walls and extracellular soluble polysaccharides (EP) released into the culture medium. Sugar composition and linkage analysis showed homogalacturonans, xylogalacturonans and arabinogalactans to be the predominant EP. In the cell wall, homogalacturonans and arabinogalactans are the major pectins, and xyloglucans and xylans are the major crosslinking glycans. The presence of xylogalacturonans in the R. jasminoides cell cultures seems to be related to the occurrence of a homogeneous cell suspension with loosely attached cells. Although all alkali extractions from the cell walls yielded amounts of xyloglucan that exceed those of the xylans, the latter was found in a proportion that is higher than what has been usually reported for primary cell walls of most eudicots. The xyloglucan from cell walls of cell suspension cultures of R. jasminoides has low fucosylation levels and high proportion of galactosyl residues, a branching pattern commonly found in storage cellwall xyloglucans.

show abstract

Differential sugar uptake by cell suspension cultures of Rudgea jasminoides, a tropical woody Rubiaceae

Cited by 17 publications

References 31 publications

Improved clonal propagation of Spilanthes acmella Murr. for production of scopoletin

Improved clonal propagation of Spilanthes acmella Murr. for production of scopoletin

Sugar metabolism ofVetiveria zizanioidescells cultured with Amberlite XAD-4. Uptake of radiolabelled sugars, cell wall invertase activity and gene expression

Cell wall polysaccharides from cell suspension cultures of the Atlantic Forest tree Rudgea jasminoides (Rubiaceae)

Contact Info

Product

Resources

About