Structure of the lipopolysaccharide O-antigen of Actinobacillus (Haemophilus) pleuropneumoniae serotype 6 (ATCC 33590)

Altman, Eleonora; Brisson, Jean‐Robert; Perry, Malcolm B.

doi:10.1139/v89-107

Cited by 8 publications

(2 citation statements)

References 9 publications

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“…pleuropneumoniae serotypes 3 , 6 , and 8 have been reported (1 8). Our structural studies show that the apparent serotype cross-reactions could be due to the common epitopic features shared by the 0-polysaccharide chains of these serotypes (6,19). The study of the crossreactions at the ultrastructural level using immunostabilization technique (20) also suggested that the observed cross-reactivity may be due to the somatic antigens.…”

Section: Resultsmentioning

confidence: 59%

Structural characterization of the capsular polysaccharide and O-chain of the lipopolysaccharide of Actinobacilluspleuropneumoniae serotype 8 (strain 405)

Altman¹,

Brisson²,

Perry³

1990

Can. J. Chem.

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The capsular polysaccharide of Actinobacilluspleuropneumoniae serotype 8 (strain 405) was found to be a teichoic acid type polysaccharide composed of 2-acetamido-2-deoxy-D-galactose (two parts), glycerol (one part), and phosphate (one part). From hydrolysis, dephosphorylation, methylation, and 1H and 13C nuclear magnetic resonance studies, the polysaccharide was found to be a high molecular weight linear polymer having the structure:[Formula: see text]The lipopolysaccharide O-antigen of A. pleuropneumoniae serotype 8 was shown to be a high molecular weight unbranched linear polymer of a repeating pentasaccharide unit having the structure identical to that produced by A. pleuropneumoniae serotype 3, as follows:[Formula: see text]Keywords: Actinobacilluspleuropneumoniae polysaccharide, lipopolysaccharide, NMR.

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Section: Resultsmentioning

confidence: 59%

Structural characterization of the capsular polysaccharide and O-chain of the lipopolysaccharide of Actinobacilluspleuropneumoniae serotype 8 (strain 405)

Altman¹,

Brisson²,

Perry³

1990

Can. J. Chem.

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“…Toutefois, quelques riactions croisies subsistent (6). Par ailleurs, ALTMAN et al (1) ont montri qu'i chaque sirotype d'A. pleuropneumoniae itait associi un LPS unique et que la diffirence entre ces LPS riside dans la structure de la chaine polysaccharidique 0.…”

Section: Discussionunclassified

Comparaison de Deux Antigénes de Nature polysaccharidique pour le Diagnostic Sérologique par ELISA de la Pleuropneumonie Porcine (Sérotype 5)

Alambédji

Dubreuil

1993

Journal of Veterinary Medicine, Series B

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Summary Comparison of two polysaccharidic antigens for ELISA serodiagnosis of pig pleuropneumonia (serotype 5) Two antigens of polysaccharidic nature were evaluated for their specificity in serodiagnosis of porcine pleuropneumonia (serotype 5). Recently, long chains lipopolysaccharides (LC‐LPS) was shown to increase the specificity of the ELISA test for A. pleuropneumoniae serotype 5 compared to a saline extract of boiled‐formalinized whole cells. Nevertheless, some cross reactions were noticed with LC‐LPS. We have thus cleaved the LPS molecule at the ketosidic bond between the lipid A and the core. The lipid A was separated from the polysaccharide (PS) by centrifugation. With an indirect sandwich ELISA, it was possible to demonstrate that the PS yielded positive responses with homologous sera (serotype 5) and negative responses with heterologous sera (serotype 3 and undeterminated serotype). Overall, the use of the PS did not notably increase the specificity of the ELISA test. Since production of PS from LC‐LPS and its separation from the lipid A is not simple, we do not recommend its use in serodiagnosis. Résumé Deux antigènes de nature polysaccharidique ont été évalués pour leur spécificité dans le diagnostic sérologique de la pleuropneumonie porcine (sérotype 5). Récemment, les lipopolysaccharides à longues chaînes (LC‐LPS), ont permis une augmentation de la spécificité par rapport à l'utilisation d'un extrait salin à chaud de cellules totales formolées. Toutefois, certaines réactions croisées ont aussi été notées avec les LC‐LPS. Nous avons donc scindé les LC‐LPS au niveau du lien cétosidique présent entre le lipide A et le core. Le polysaccharide (PS), constitué du core et de la chaîne O, fut alors séparé du lipide A par centrifugation. Grâce à un test ELISA sandwich indirect, il a été possible de démontrer que le PS donnait des réponses positives avec les sérums homologues (antisérotype 5) et des réponses négatives avec des sérums hétérologues (sérotype 3 et indéterminé). En résumé, l'utilisation du PS n'a pas sensiblement amélioré la spécificité de l'épreuve ELISA. L'hydrolyse du LC‐LPS et la séparation du PS du lipide A étant laborieuse et complexe, nous ne recommandons pas son utilisation pour le diagnostic sérologique.

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Structure of the capsular polysaccharide from Actinobacillus pleuropneumoniae serotype 10

Beynon

Richards

Perry

1991

Carbohydrate Research

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The capsular polysaccharide of A. pleuropneumoniae serotype 10 was found to be composed of a linear disaccharide repeating unit. By use of methylation analysis, partial hydrolysis, g.l.c.--and f.a.b.- m.s., and 1D and 2D n.m.r. studies, the polysaccharide was determined to be a polymer of a repeating disaccharide unit composed of D-mannose and 3-deoxy-D-manno-octulosonic acid (Kdo) having the structure: [formula; see text]

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Structure of the lipopolysaccharide O-antigen of Actinobacillus (Haemophilus) pleuropneumoniae serotype 6 (ATCC 33590)

Cited by 8 publications

References 9 publications

Structural characterization of the capsular polysaccharide and O-chain of the lipopolysaccharide of Actinobacilluspleuropneumoniae serotype 8 (strain 405)

Structural characterization of the capsular polysaccharide and O-chain of the lipopolysaccharide of Actinobacilluspleuropneumoniae serotype 8 (strain 405)

Comparaison de Deux Antigénes de Nature polysaccharidique pour le Diagnostic Sérologique par ELISA de la Pleuropneumonie Porcine (Sérotype 5)

Structure of the capsular polysaccharide from Actinobacillus pleuropneumoniae serotype 10

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