Structure of the capsular polysaccharide from Actinobacillus pleuropneumoniae serotype 10

Abstract

The capsular polysaccharide of A. pleuropneumoniae serotype 10 was found to be composed of a linear disaccharide repeating unit. By use of methylation analysis, partial hydrolysis, g.l.c.--and f.a.b.- m.s., and 1D and 2D n.m.r. studies, the polysaccharide was determined to be a polymer of a repeating disaccharide unit composed of D-mannose and 3-deoxy-D-manno-octulosonic acid (Kdo) having the structure: [formula; see text]

Characterization of the capsular antigen of Streptococcus pneumoniae serotype 35B

“…Two dimensional homonuclear chemical shift correlated (COSY) experiments were camed out as previously described (19). The heteronuclear I3c-'~ chemical shift correlations were measured in the 'H-detected mode via multiple quantum coherence ('H('~c)HMQc) with a Bruker 5-mm inverse broadband probe using reverse electronics as previously described (20).…”

Polysaccharide biological response modifiers

“…In general, CPs are composed of single monosaccharide repeating units [18] or oligosaccharide repeating subunits. The oligosaccharide repeating unit can be disaccharide [19], trisaccharide [20][21][22], tetrasaccharide [23,24], pentasaccharide [25][26][27][28][29][30], and hexasaccharide [31][32][33]. The monosaccharides are usually modified with N-acetylation [31][32][33][34][35], carboxylation [31][32][33][34][35], and occasionally phosphorylation [32].…”