Structural characterization of the capsular polysaccharide and O-chain of the lipopolysaccharide of Actinobacilluspleuropneumoniae serotype 8 (strain 405)

Abstract

The capsular polysaccharide of Actinobacilluspleuropneumoniae serotype 8 (strain 405) was found to be a teichoic acid type polysaccharide composed of 2-acetamido-2-deoxy-D-galactose (two parts), glycerol (one part), and phosphate (one part). From hydrolysis, dephosphorylation, methylation, and 1H and 13C nuclear magnetic resonance studies, the polysaccharide was found to be a high molecular weight linear polymer having the structure:[Formula: see text]The lipopolysaccharide O-antigen of A. pleuropneumoniae serotype 8 was shown to be a high molecular weight unbranched linear polymer of a repeating pentasaccharide unit having the structure identical to that produced by A. pleuropneumoniae serotype 3, as follows:[Formula: see text]Keywords: Actinobacilluspleuropneumoniae polysaccharide, lipopolysaccharide, NMR.

Clinical Efficacy and Residue Depletion of 10% Enrofloxacin Enteric-Coated Granules in Pigs

“…There are 12 serotypes of biotype 1, and 6 serotypes of biotype 2, which are defined based on their surface polysaccharide antigens (Altman et al, 1990; Perry et al, 1990; Schaller et al, 2001; Bossé et al, 2002). All serotypes can be urease positive, and thus cause respiratory infection and serious economic loss.…”

“…Actinobacillus pleuropneumonia (APP) is an obligate parasite of the porcine respiratory tract that can infect nasal cavities and tonsils of pigs (Dom et al, 1994a ; Duff et al, 1996 ; Yoshimura et al, 2002 ; Jobert et al, 2010 ). There are 12 serotypes of biotype 1, and 6 serotypes of biotype 2, which are defined based on their surface polysaccharide antigens (Altman et al, 1990 ; Perry et al, 1990 ; Schaller et al, 2001 ; Bossé et al, 2002 ). All serotypes can be urease positive, and thus cause respiratory infection and serious economic loss.…”

Syntheses of β‐C(1→3)‐Glucopyranosides of 2‐ and 4‐Deoxy‐D‐hexoses

“…The latter operation was repeated twice and the residue purified by FC (silica gel, light petroleum ether/AcOEt 1 : 2): 138 mg of 9 (56%, 2 steps) (6)); 3.60 (m, HÀC(6')); 2.24 (m, HÀC (3)); 2.13 ± 1.99 (19 H, H eq ÀC(2), 6 Ac); 1.53 (dd, 2 J 14.8, H ax ÀC(2)). 1 (10). A mixture of 9 (130 mg, 0.23 mmol), Ac 2 O (4.7 ml), and CF 3 COOH (1.1 ml) was stirred at 208 for 2 h, then poured onto ice (10 ml), and neutralized with sat.…”

“…This motif, for which 1 is a mimetic, is also found in the glucan fragment responsible for triggering the defense of the soybean to infections by the mould Phytophthora megasperma [9]. The lipopolysaccharide O-antigens of Actinobacillus pleuropneumania serotype 8 contains the b-d-Glcp-(1 3 3)-b-d-Galf motif [10] for which 2' is a mimetic 1 …”