Structural and Biological Assessment of Zinc Doped Hydroxyapatite Nanoparticles

Popa, Claudia; Aur,; Deniaud, lien; Michaud‐Soret, Isabelle; Gu, gis; Gan, Mohan; Motelica-Heino, Mikaël; Predoi, Daniela

doi:10.1155/2016/1062878

Cited by 63 publications

(58 citation statements)

References 41 publications

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“…The crystallite size of the prepared HAp obtained from Scherrer’s equation was 83.5 ± 1 nm. Similar results were also obtained in our previous studies [ 61 , 62 ]. According to A. Bigi et al [ 62 ], the values of the parameters of the sides “a” and “c” are influenced both by the synthesis method, and the pH at which synthesis was achieved.…”

Section: Resultssupporting

confidence: 93%

Antimicrobial Activity of New Materials Based on Lavender and Basil Essential Oils and Hydroxyapatite

et al. 2018

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This study presents, for the first-time, the results of a study on the hydrodynamic diameter of essential oils (EOs) of basil and lavender in water, and solutions of EOs of basil (B) and lavender (L) and hydroxyapatite (HAp). The possible influence of basil and lavender EOs on the size of hydroxyapatite nanoparticles was analyzed by Scanning Electron Microscopy (SEM). We also investigated the in vitro antimicrobial activity of plant EOs and plant EOs hydroxyapatite respectively, against Gram-positive bacteria (methicillin-resistant Staphylococcus aureus1144 (MRSA 1144) and S. aureus 1426) and Gram-negative bacteria (Escherichia coli ATCC 25922 and Escherichia coli ESBL 4493). From the autocorrelation function, obtained by Dynamic Light Scattering (DLS) measurements it was observed that basil yielded one peak at an average hydrodynamic diameter of 354.16 nm, while lavender yielded one peak at an average hydrodynamic diameter of 259.76 nm. In the case of HAp nanoparticles coated with basil (HApB) and lavender (HApL) essential oil, the aggregation was minimal. We found that the lavender EO exhibited a very good inhibitory growth activity (MIC values ranging from <0.1% for E. coli reference strain to 0.78% for S. aureus strains). The biological studies indicated that HapL material displayed an enhanced antimicrobial activity, indicating the potential use of HAp as vehicle for low concentrations of lavender EO with antibacterial properties. Flow cytometry analysis (FCM) allowed us to determine some of the potential mechanisms of the antimicrobial activities of EOs, suggesting that lavender EO was active against E. coli by interfering with membrane potential, the membrane depolarization effect being increased by incorporation of the EOs into the microporous structure of HAp. These findings could contribute to the development of new antimicrobial agents that are urgently needed for combating the antibiotic resistance phenomena.

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Section: Resultssupporting

confidence: 93%

Antimicrobial Activity of New Materials Based on Lavender and Basil Essential Oils and Hydroxyapatite

et al. 2018

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“…Certain studies have examined the effect of other thin layers on C. albicans [ 14 ]. Recent research regarding the effect of antimicrobial activity of Zn:HAp nanoparticles is available [ 25 , 26 ]. In recent studies on textural, structural and biological evaluation of hydroxyapatite doped with zinc at low concentrations, Predoi et al [ 25 ] assessed the effect of Zn:HAp on cell viability on prokaryote and eukaryote cells.…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, the cytotoxic effects were intense in the case of hepatic cells. Furthermore, the studies on structural and biological assessment of zinc doped hydroxyapatite nanoparticles conducted by Popa et al [ 26 ] concluded that that “Zn doping at the tested concentrations did not induce a specific prokaryote or eukaryote toxicity in HAp compounds” and clearly showed that the activity of Zn:HAp nanoparticles against HepG2 cells was meaningfully dependent on the particle size. Another study on flower-shaped ZnO nanoparticles synthesized by a novel approach at near-room temperatures with antibacterial and antifungal properties conducted by Khan et al [ 35 ] showed that the antimicrobial effects can be influenced by the shape of nanoparticles.…”

Section: Resultsmentioning

confidence: 99%

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Structural Characterization and Antifungal Studies of Zinc-Doped Hydroxyapatite Coatings

et al. 2017

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The present study is focused on the synthesis, characterization and antifungal evaluation of zinc-doped hydroxyapatite (Zn:HAp) coatings. The Zn:HAp coatings were deposited on a pure Si (Zn:HAp_Si) and Ti (Zn:HAp_Ti) substrate by a sol-gel dip coating method using a zinc-doped hydroxyapatite nanogel. The nature of the crystal phase was determined by X-ray diffraction (XRD). The crystalline phase of the prepared Zn:HAp composite was assigned to hexagonal hydroxyapatite in the P63/m space group. The colloidal properties of the resulting Zn:HAp (xZn = 0.1) nanogel were analyzed by Dynamic Light Scattering (DLS) and zeta potential. Scanning Electron Microscopy (SEM) was used to investigate the morphology of the zinc-doped hydroxyapatite (Zn:HAp) nanogel composite and Zn:HAp coatings. The elements Ca, P, O and Zn were found in the Zn:HAp composite. According to the EDX results, the degree of Zn substitution in the structure of Zn:HAp composite was 1.67 wt%. Moreover, the antifungal activity of Zn:HAp_Si and Zn:HAp_Ti against Candida albicans (C. albicans) was evaluated. A decrease in the number of surviving cells was not observed under dark conditions, whereas under daylight and UV light illumination a major decrease in the number of surviving cells was observed.

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“…Metal ions such as Ag + , Zn 2+ , and Cu 2+ can be transported by HAp through ionic substitution. The anterior results showed that the Ag + and Zn 2+ affect the development of microorganisms . In contrast, in the precedent studies conducted on bacterial resistance to silver in wound care revealed that the Ag + show oligodynamic effect with minimum development of resistant microorganisms.…”

Section: Resultsmentioning

confidence: 89%

Physicochemical and antimicrobial properties of silver‐doped hydroxyapatite collagen biocomposite

Predoi

Iconaru

Albu

et al. 2017

Polymer Engineering & Sci

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Silver‐doped hydroxyapatite (AgHAp) was prepared by co‐precipitation method at room temperature. The obtained AgHAp was added in different amounts of collagen gel (AgHApC1 and AgHApC2). Afterward, the gel was lyophilized and the final AgHApC1 and AgHApC2 composite was achieved. The purity, crystallinity, and the phase composition of the AgHAp, AgHApC1, and AgHApC2 samples were evaluated by X‐ray diffraction (XRD). The planes corresponding to the 2θ values for hydroxyapatite were found in the three samples analyzed in agreement with the crystalline hydroxyapatite. In the Fourier transform infrared (FT‐IR) spectra of AgHApC1 and AgHApC2 samples the peak characteristic to the presence of ν2 phosphate mode at 472/cm was found. The peaks resulting from the ν4 vibration of the P–O mode, ν1 symmetric P–O stretching vibration and ν3 P–O stretching vibration of PO43− were also evidenced in all the samples. The formation of agglomerated particles with uniform particle size was evidenced by scanning electron microscope (SEM). The uniform distribution of the constituent elements was evidenced by mapping analysis. Furthermore, the strong antibacterial activity of AgHAp, AgHApC1, and AgHApC2 samples against Gram‐negative (Escherichia coli) and Gram‐positive (Staphylococcus aureus) bacterial strains was shown. The inhibition zone increased drastically with the increase of silver concentration. POLYM. ENG. SCI., 57:537–545, 2017. © 2017 Society of Plastics Engineers

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Structural and Biological Assessment of Zinc Doped Hydroxyapatite Nanoparticles

Cited by 63 publications

References 41 publications

Antimicrobial Activity of New Materials Based on Lavender and Basil Essential Oils and Hydroxyapatite

Antimicrobial Activity of New Materials Based on Lavender and Basil Essential Oils and Hydroxyapatite

Structural Characterization and Antifungal Studies of Zinc-Doped Hydroxyapatite Coatings

Physicochemical and antimicrobial properties of silver‐doped hydroxyapatite collagen biocomposite

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