Physicochemical and antimicrobial properties of silver‐doped hydroxyapatite collagen biocomposite

Abstract

Silver‐doped hydroxyapatite (AgHAp) was prepared by co‐precipitation method at room temperature. The obtained AgHAp was added in different amounts of collagen gel (AgHApC1 and AgHApC2). Afterward, the gel was lyophilized and the final AgHApC1 and AgHApC2 composite was achieved. The purity, crystallinity, and the phase composition of the AgHAp, AgHApC1, and AgHApC2 samples were evaluated by X‐ray diffraction (XRD). The planes corresponding to the 2θ values for hydroxyapatite were found in the three samples analyzed in agreement with the crystalline hydroxyapatite. In the Fourier transform infrared (FT‐IR) spectra of AgHApC1 and AgHApC2 samples the peak characteristic to the presence of ν2 phosphate mode at 472/cm was found. The peaks resulting from the ν4 vibration of the P–O mode, ν1 symmetric P–O stretching vibration and ν3 P–O stretching vibration of PO43− were also evidenced in all the samples. The formation of agglomerated particles with uniform particle size was evidenced by scanning electron microscope (SEM). The uniform distribution of the constituent elements was evidenced by mapping analysis. Furthermore, the strong antibacterial activity of AgHAp, AgHApC1, and AgHApC2 samples against Gram‐negative (Escherichia coli) and Gram‐positive (Staphylococcus aureus) bacterial strains was shown. The inhibition zone increased drastically with the increase of silver concentration. POLYM. ENG. SCI., 57:537–545, 2017. © 2017 Society of Plastics Engineers

Physico-Chemical Properties and In Vitro Antifungal Evaluation of Samarium Doped Hydroxyapatite Coatings

“…In addition, due to its hexagonal structure, HAp on the one hand has the capacity to incorporate in its structure various ions (Eu 3+ , Ag + , Mg 2+ , Zn 2+ , and Sm 3+ , etc.) [10][11][12] and on the other hand the HAp surface can be modified with various biopolymers/drugs [13], which leads to an improvement of the biological and physico-chemical properties, thus making HAp more efficient and useful for the medical sphere [2]. Hydroxyapatite can be obtained in various forms, from powder/gel and reaching to thin layers/coatings by various synthesis methods, among which we mention the sol-gel method, coprecipitation (these are often used to obtain powders/gels), dip coating, spin coating, magnetron sputering and so forth (the latter being used to obtain thin HAp coatings) [14].…”

Review of the Applications of Biomedical Compositions Containing Hydroxyapatite and Collagen Modified by Bioactive Components

“…Ternary systems based on collagen, hydroxyapatite, and silver have been proposed by Predoi et al [ 218 ] Silver-doped hydroxyapatite has been prepared via a coprecipitation at room temperature. Calcium nitrate and ammonium hydrogen phosphate have been used as HAp precursors, and silver nitrate has been used as a source of silver.…”

“…In both cases, an increase in the inhibition zone with an increasing concentration of the silver in the tested material was demonstrated. Due to the simplicity of the synthesis methodology applied, the preparation of a homogeneous distribution of Ag-HAp in the collagen, as well as the antibacterial activity of obtained materials of such conjugates, have been considered as interesting and promising for application in the regeneration of bone tissue [ 218 ].…”

In vitro characterization of novel nanostructured collagen-hydroxyapatite composite scaffolds doped with magnesium with improved biodegradation rate for hard tissue regeneration

“…The Ag containing compound exhibited enhanced mechanical properties and good antibacterial ability along with biocompatibility towards MG-63 and red blood cells. Predoi et al [ 16 ] reported a study where a composite of Ag-substituted HAp (0.1% Ag) was added to a collagen gel. Also in this case, the Ag-containing biocomposite showed antibacterial characteristics.…”