Antimicrobial Activity of New Materials Based on Lavender and Basil Essential Oils and Hydroxyapatite

Abstract

This study presents, for the first-time, the results of a study on the hydrodynamic diameter of essential oils (EOs) of basil and lavender in water, and solutions of EOs of basil (B) and lavender (L) and hydroxyapatite (HAp). The possible influence of basil and lavender EOs on the size of hydroxyapatite nanoparticles was analyzed by Scanning Electron Microscopy (SEM). We also investigated the in vitro antimicrobial activity of plant EOs and plant EOs hydroxyapatite respectively, against Gram-positive bacteria (methicillin-resistant Staphylococcus aureus1144 (MRSA 1144) and S. aureus 1426) and Gram-negative bacteria (Escherichia coli ATCC 25922 and Escherichia coli ESBL 4493). From the autocorrelation function, obtained by Dynamic Light Scattering (DLS) measurements it was observed that basil yielded one peak at an average hydrodynamic diameter of 354.16 nm, while lavender yielded one peak at an average hydrodynamic diameter of 259.76 nm. In the case of HAp nanoparticles coated with basil (HApB) and lavender (HApL) essential oil, the aggregation was minimal. We found that the lavender EO exhibited a very good inhibitory growth activity (MIC values ranging from <0.1% for E. coli reference strain to 0.78% for S. aureus strains). The biological studies indicated that HapL material displayed an enhanced antimicrobial activity, indicating the potential use of HAp as vehicle for low concentrations of lavender EO with antibacterial properties. Flow cytometry analysis (FCM) allowed us to determine some of the potential mechanisms of the antimicrobial activities of EOs, suggesting that lavender EO was active against E. coli by interfering with membrane potential, the membrane depolarization effect being increased by incorporation of the EOs into the microporous structure of HAp. These findings could contribute to the development of new antimicrobial agents that are urgently needed for combating the antibiotic resistance phenomena.

Multifunctional Hydroxyapatite Coated with Arthemisia absinthium Composites

“…The results highlighted that the use of DMSO as solvent did not affect bacterial growth. The results regarding the antimicrobial activity of HAp solution and DMSO are in agreement with previously reported studies by the authors [20,25]. Even though, the pharmacologic effects of DMSO are diverse and it has been reported to present anti-inflammatory, cryopreservative, antiischemic, radioprotective qualities and even antiseptic properties [37] its effect on microorganisms depends on numerous factors such as microbial strain, concentration, pH, etc [38].…”

“…The measurements were conducted as previously described in Predoi et al [35]. Additionally, a Perkin-Elmer Turbomass Quadrupole mass spectrometer (PerkinElmer Inc., Waltham, MA, USA) was used for the GC/MS studies [20]. The chemical elements of the studies sample were identified using NIST and Wiley Registry 8 Edition mass database, n-alkalene (C 9 -C 22 ) hydrocarbon series (Nile, Italy).…”

“…Besides, wormwood oil and aqueous extracts hold worthy anti-inflammatory and antinociceptive properties-more like analgesic actions [19]. Being an excellent biocompatible and bioactive nanomaterial, hydroxyapatite (also known as HAp) is endorsed by biological studies as being a worthy carrier for low concentrations of essential oils, thus exhibiting enhanced antimicrobial actions [20].…”

Zinc Doped Hydroxyapatite Thin Films Prepared by Sol–Gel Spin Coating Procedure

“…These days, one of the most distressing complications that could appear after surgery is considered the apparition of a post operatory infection [5,6]. In most cases, the infection appears because of the bacterial cells, which adhere either to an implantable medical device (when is the case), either on the tissue surrounding the surface of the implant, or simply on the surface of the wound [7,8]. These types of bacterial adhesions on implantable devices are some of the most frightening problems that can occur in the dentistry, orthopedic, and cardiovascular field, because they usually lead to severe life treating infections due to the development The morphology of the samples was investigated by scanning electron microscopy (SEM), using a HITACHI S4500 microscope (Hitachi, Ltd., Tokyo, Japan) equipped with an X-ray energy dispersive spectroscopy (EDX) system.…”

Increased UV absorption properties of natural hydroxyapatite‐based sunscreen through laser ablation modification in liquid

“…This can be explained considering some agglomeration of the particles; indeed such agglomeration and the difference between SEM and DLS results was previously observed for HAp‐based nanomaterial. The agglomeration can be due to the OH groups present in the HAp molecule and the hydrogen bond‐type interactions; the presence of iron ion in the powders also seem to favor the agglomeration …”