Zinc Doped Hydroxyapatite Thin Films Prepared by Sol–Gel Spin Coating Procedure

Predoi, Daniela; Iconaru, Simona Liliana; Predoi, Mihai Valentin; Buton, Nicolas; Motelica-Heino, Mikaël

doi:10.3390/coatings9030156

Cited by 40 publications

(32 citation statements)

References 55 publications

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“…The maximum inhibitory concentration of Zn 2%-HA to Gram-positive bacteria and Gram-negative bacteria was 50 ± 5% and 77 ± 5%, respectively [44]. The antimicrobial effect of Zn-HA layer on the growth of candida albicans showed that even after 8 h of incubation, the growth of fungal cells was inhibited and weakened with time [45]. Therefore, the use of Zn-HA in bone tissue engineering scaffolds has the effect of antibacterial and regulating immune cells, which can increase the value of scaffold materials.…”

Section: Zinc Doped Hydroxyapatite (Zn-ha)mentioning

confidence: 97%

Hydroxyapatite Based Materials for Bone Tissue Engineering: A Brief and Comprehensive Introduction

et al. 2021

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Hydroxyapatite (HA) is widely used in bone tissue engineering for its bioactivity and biocompatibility, and a growing number of researchers are exploring ways to improve the physical properties and biological functions of hydroxyapatite. Up to now, HA has been used as inorganic building blocks for tissue engineering or as nanofillers to blend with polymers, furthermore, various methods such as ion doping or surface modification have been also reported to prepare functionalized HA. In this review, we try to give a brief and comprehensive introduction about HA-based materials, including ion-doped HA, HA/polymer composites and surface modified HA and their applications in bone tissue engineering. In addition, the prospective of HA is also discussed. This review may be helpful for researchers to get a general understanding about the development of hydroxyapatite based materials.

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Section: Zinc Doped Hydroxyapatite (Zn-ha)mentioning

confidence: 97%

Hydroxyapatite Based Materials for Bone Tissue Engineering: A Brief and Comprehensive Introduction

et al. 2021

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“…The quantitative assays of the antifungal activity of both 5SmHAp suspensions and coatings were done using an adapted method (E2149-10; ASTM International) [30,31], as previously described [32]. The experiments were performed in triplicate and the results of the results were expressed as mean ± SD.…”

Section: In Vitro Antifungal Activitymentioning

confidence: 99%

Physico-Chemical Properties and In Vitro Antifungal Evaluation of Samarium Doped Hydroxyapatite Coatings

et al. 2020

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Hydroxyapatite (HAp) and samarium doped hydroxyapatite, Ca10−xSmx(PO4)6(OH)2, xSm = 0.05, (5SmHAp), coatings were prepared by sol-gel process using the dip coating method. The stability of 5SmHAp suspension was evaluated by ultrasound measurements. Fourier transform infrared spectroscopy (FTIR) was used to examine the optical characteristics of HAp and 5SmHAp nanoparticles in suspension and coatings. The FTIR analysis revealed the presence of the functional groups specific to the structure of hydroxyapatite in the 5SmHAp suspensions and coatings. The morphology of 5SmHAp nanoparticles in suspension was evaluated by transmission electron microscopy (TEM). Moreover, scanning electron microscope (SEM) was used to evaluate the morphology of nanoparticle in suspension and the morphology of the surface on the coating. The SEM and TEM studies on 5SmHAp nanoparticles in suspension showed that our samples consist of nanometric particles with elongated morphology. The SEM micrographs of HAp and 5SmHAp coatings pointed out that the coatings are continuous and homogeneous. The surface morphology of the 5SmHAp coatings was also assessed by Atomic Force Microscopy (AFM) studies. The AFM results emphasized that the coatings presented the morphology of a uniformly deposited layer with no cracks and fissures. The crystal structure of 5SmHAp coating was characterized by X-ray diffraction (XRD). The surface composition of 5SmHAp coating was analyzed by X-ray photoelectron spectroscopy (XPS). The XRD and XPS analysis shown that the Sm3+ ions have been incorporated into the 5SmHAp synthesized material. The antifungal properties of the 5SmHAp suspensions and coatings were studied using Candida albicans ATCC 10231 (C. albicans) fungal strains. The quantitative results of the antifungal assay showed that colony forming unity development was inhibited from the early phase of adherence in the case of both suspensions and coatings. Furthermore, the adhesion, cell proliferation and biofilm formation of the C. albicans were also investigated by AFM, SEM and Confocal Laser Scanning Microscopy (CLSM) techniques. The results highlighted that the C. albicans adhesion and cell development was inhibited by the 5SmHAp coatings. Moreover, the data also revealed that the 5SmHAp coatings were effective in stopping the biofilm formation on their surface. The toxicity of the 5SmHap was also investigated in vitro using HeLa cell line.

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“…The antifungal activity of HApAg5 and HApAg20 suspensions and thin layers was also evaluated by a quantitative assay. The quantitative assays of the antifungal properties of HApAg5 and HApAg20 suspensions and thin layers after 24 and 48 h was performed using an adapted method (E2149-10; ASTM International) [29,30] previously described in Predoi et al [31]. The experiments were done in triplicate and the results were presented as mean ±SD.…”

Section: In Vitro Antifungal Assaysmentioning

confidence: 99%

Silver-Doped Hydroxyapatite Thin Layers Obtained by Sol-Gel Spin Coating Procedure

et al. 2019

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The main objective of this paper is to develop silver-doped hydroxyapatite suspensions (HApAg) with different concentrations of silver (?Ag = 0.05 and ?Ag = 0.2) in order to obtain uniform and homogenous layers by spin-coating procedure. The colloidal properties of HApAg suspensions are evaluated by dynamic light scattering (DLS) analysis, ζ-potential (ZP), and ultrasound measurements. The ultrasound studies show that the HApAg20 sample revealed better stability than the HApAg5 sample. The structural and morphological analysis on suspensions and thin layers is also conducted. It is observed that the particles of the two samples have a similar shape and are uniform. The layers obtained present a homogeneous appearance of the surface without evidence of cracks or interruption of the coatings. The in vitro antifungal studies conducted on the two thin layers at two different time intervals (24 and 48 h) show that both HApAg5 (?Ag = 0.05) and HApAg20 (?Ag = 0.05) nanoparticles suspensions and composite layers inhibit the development of colony forming units (CFU) even after 24 h of incubation comparative to the control, represented by the Candida albicans (C. albicans) culture in a proper medium. The fungicidal effect was evident after 48 h of incubation in the case of both HApAg20 nanoparticles suspensions and composite layers.

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Zinc Doped Hydroxyapatite Thin Films Prepared by Sol–Gel Spin Coating Procedure

Cited by 40 publications

References 55 publications

Hydroxyapatite Based Materials for Bone Tissue Engineering: A Brief and Comprehensive Introduction

Hydroxyapatite Based Materials for Bone Tissue Engineering: A Brief and Comprehensive Introduction

Physico-Chemical Properties and In Vitro Antifungal Evaluation of Samarium Doped Hydroxyapatite Coatings

Silver-Doped Hydroxyapatite Thin Layers Obtained by Sol-Gel Spin Coating Procedure

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