Streptomyces chilikensis sp. nov., a halophilic streptomycete isolated from brackish water sediment

Abstract

A novel actinobacterial strain, designated RC 1830 T , was isolated from the sediment of estuarine coastal brackish water lagoon of Chilika Lake, in Khurdha district of Odisha, India, and characterized using a polyphasic approach. Strain RC 1830 T was halophilic and alkali-tolerant and found to hydrolyse chitin, starch, tributyrin, lecithin, Tween 80, cellulose, gelatin and casein. The diagnostic presence of LL-diaminopimelic acid, iso-C 15 : 0 , anteiso-C 15 : 0 , iso-C 16 : 0 , C 16 : 0 , iso-C 17 : 0 , anteiso-C 17 : 0 as major cellular fatty acids and MK-9(H 4 and H 6 ) as major menaquinones noticeably associated the strain to the genus Streptomyces. After comparison and analysis of the near complete 16S rRNA gene sequence with representative strains of other streptomycetes, it was evident that strain RC 1830 T belonged to the genus Streptomyces, and exhibited the highest sequence similarities of 99.53 %, 99.25 %, 99.11 %, 99.10 % and 99. 06 % to Streptomyces fragilis DSM 40044 T , Streptomyces coelicoflavus NBRC 15399 T , Streptomyces flaveolus NBRC 3715 T , Streptomyces lavenduligrisesus NBRC 13405 T and Streptomyces eurythermus ATCC 14975 T , respectively. Reconstruction of a phylogenetic tree for the genus Streptomyces revealed that strain RC 1830 T formed a distinct phyletic line and clustered with its most closely related neighbour S. fragilis DSM 40044 T . The DNA-DNA relatedness values between strain RC 1830 T and the most closely related type strain S. fragilis DSM 40044 T were determined to be 17.7±4.55 %. Additionally, morphological, biochemical and physiological tests were able to distinguish the strain from the most closely related type strain S. fragilis DSM 40044 T and other closely related neighbours, S. coelicoflavus DSM 41471 T and Streptomyces flaveolus DSM 40061 T . Based on a range of phenotypic and genotypic properties, strain RC 1830 T is suggested to represent a novel species of the genus Streptomyces for which the name Streptomyces chilikensis sp. nov. is proposed. The type strain is RC 1830 T (5JCM 18411 T 5DSM 42072 T ).

Streptomyces barkulensis sp. nov., isolated from an estuarine lake

“…The pure strain was maintained routinely on agar plates of modified Luria–Bertini (LB) media fortified with seawater (SWLB; 10.0 g tryptone, 5.0 g yeast extract, 5.0 g NaCl, 500 ml seawater, 500 ml distilled water, 18 g agar, pH 7.0) at 4 °C and as a suspension of mycelia and spores in 50 % (v/v) glycerol at −80 °C. Biomass required for chemotaxonomic and molecular analyses was obtained by culturing isolate RC 1831 T in SWLB for 48 h at 30 °C and cells were harvested by centrifugation and resuspended in water/2-propanol (1 : 1, v/v) (Ray et al , 2013).…”

Pyrosequencing based profiling of the bacterial community in the Chilika Lake, the largest lagoon of India

“…nov., a moderately alkaliphilic gammaproteobacterium [7] , Streptomyces chilikensis sp. nov., a halophilic streptomycete [8] and Streptomyces barkulensis sp. nov [9] have also been isolated from the Chilika Lake.…”

Biodiversity and biotechnological applications of halophilic microbes for sustainable agriculture

“…The novel and efficient enzymes producing halophilic microbes may be used in different industrial processes. There are many novel species of halophilic microbes including Actinopolyspora mortivallis [3], Halothermothrix orenii [4], Natrinema versiforme [5], Halomonas marisflavae [6], Bacillus marisflavi [7], Arcobacter halophilus [8], Nesterenkonia aethiopica [9], Aquisalimonas asiatica [10], Amycolatopsis halophila [11], Pontibacillus yanchengensis [12], Streptomyces chilikensis [13], Prauserella isguenensis [14], Marinirhabdus gelatinilytica [15], and Haloprofundus marisrubri [16], which have been sorted out from crops growing under saline conditions, saline lakes, and hypersaline soils worldwide.…”