Streptomyces barkulensis sp. nov., isolated from an estuarine lake

Abstract

The taxonomic position of a novel actinomycete, strain RC 1831 T , isolated from the sediment of a fish dumping yard at Barkul village near Chilika Lake, Odisha, India, was determined by a polyphasic approach. Based on morphological and chemotaxonomic characteristics the isolate was determined to belong to the genus Streptomyces. The phylogenetic tree based on its nearly complete 16S rRNA gene sequence (1428 nt) with representative strains showed that the strain consistently falls into a distinct phyletic line together with Streptomyces glaucosporus DSM 41689 T (98.22 % similarity) and a subclade consisting of Streptomyces atacamensis DSM 42065 T (98.40 %), Streptomyces radiopugnans R97 DSM 41901 T (98.27 %), Streptomyces fenghuangensis GIMN4.003 T (98.33 %), Streptomyces nanhaiensis DSM 41926 T (98.13 %), Streptomyces megasporus NBRC 14749 T (97.37 %) and Streptomyces macrosporus NBRC 14748 T (98.22 %). However, the levels of DNA-DNA relatedness between strain RC 1831 T and phylogenetically related strains Streptomyces atacamensis DSM 42065 T (28.75±3.25 %) and Streptomyces glaucosporus DSM 41689 T (15±2.40 %) were significantly lower than the 70 % threshold value for delineation of genomic species. Furthermore, the isolate could be distinguished phenotypically on the basis of physiological, morphological and biochemical differences from its closest phylogenetic neighbours and other related reference strains. Strain RC 1831 T is therefore considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces barkulensis sp. nov. is proposed. The type strain is RC 1831 T (5JCM 18754 T 5DSM 42082 T ).

Streptomyces mangrovi sp. nov., isolated from mangrove forest sediment

“…1 Scanning electron micrograph of isolate GY1 T on yeast extract-malt extract agar after 14 days at 28°C showing rough to warty ornamented spores in spiral chains. Indeed, similar fatty acid profiles have been reported for the type strains of S. atacamensis, S. barkulensis and S. radiopugnans (Mao et al 2007;Santhanam et al 2012;Ray et al 2014). Similar fatty acid patterns were shown to be present in S. fenghuangensis NRRL B-24801 T and S. S. nanhaiensis KCTC 19401 T although quantitative differences were found in the proportions of the major components and small qualitative differences in some fatty acids found in minor and trace amounts (Supplementary Table S1), results in good agreement with previous fatty acid analyses of these organisms (Zhu et al 2011;Tian et al 2012).…”

“…Isolate GY1 T , unlike the type strain of S. fenghuangensis, its nearest phylogenetic neighbour, was found to form spores in spiral chains and to use sucrose, trehalose and xylose as sole carbon sources. Similarly, isolate GY1 T can be distinguished from the type strain of S. barkulensis using a combination of phenotypic features acquired using the same standard taxonomic methods (Ray et al 2014). Similarly, isolate GY1 T can be distinguished from the type strain of S. barkulensis using a combination of phenotypic features acquired using the same standard taxonomic methods (Ray et al 2014).…”

Streptomyces verrucosisporus sp. nov., isolated from marine sediments

“…In addition, Streptomyces fenghuangensis NRRL B-24801 T contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, one unidentified aminolipid, two unidentified phospholipids and an unidentified glycolipid (Fig. S1), which is similar to the polar lipids of 'Streptomyces mangrovicola' GY1 (=NCIMB 14980) (Yousif et al, 2015), Streptomyces barkulensis RC 1831 T (=JCM 18754 T ) (Ray et al, 2014) and Streptomyces radiopugnans R97…”

“…In addition, the presence of lysophosphatidylethanolamine in the phospholipid profile was distinctive from Streptomyces fenghuangensis NRRL B-24801 T and related type strains ( Fig. S1; Yousif et al, 2015;Ray et al, 2014;Mao et al, 2007). According to Ray et al (2014), growth of Streptomyces barkulensis RC 1831 T was not observed on ISP2 agar medium.…”

“…S1; Yousif et al, 2015;Ray et al, 2014;Mao et al, 2007). According to Ray et al (2014), growth of Streptomyces barkulensis RC 1831 T was not observed on ISP2 agar medium. However, in our present study this type strain grew well on ISP2 agar (Table S1).…”