Two actinomycete strains, KK1-2 T and CPB4-7, were isolated from marine sediments collected inChumphon province, Thailand. Chumphon province, Thailand. Their taxonomic positions were determined using a polyphasic approach. The morphological, cultural and chemotaxonomic characteristics of these isolates were consistent with the classification of the strains as representing a member of the genus Streptomyces. They contained LL-diaminopimelic acid in their cell wall peptidoglycan; the whole-cell sugars were ribose and glucose. The predominant menaquinones were MK9-(H 6 ) and MK9-(H 8 ). The major polar lipids were phosphatidylethanolamine, phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides. The predominant cellular fatty acids were anteiso-C 15 : 0 , iso-C 16 : 0 and iso-C 15 : 0 . On the basis of 16S rRNA gene sequence similarity studies, these isolates were determined to be closely related to
A novel actinomycete, strain RCU-197 T , was isolated from soil of a peat swamp forest in Rayong Province, Thailand. Using a polyphasic approach, the strain was classified in the genus Streptomyces. It contained LL-diaminopimelic acid in the cell-wall peptidoglycan. No diagnostic sugars were detected in whole-cell hydrolysates and there was a lack of mycolic acids. The major menaquinones were MK-9(H 6 ) and MK-9(H 8 ). The predominant cellular fatty acids were iso-C 14 : 0 , iso-C 15 : 0 , anteiso-C 15 : 0 and iso-C 16 : 0 . The polar lipids profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and phosphatidylinositol mannoside, an unknown aminolipid and two unknown phospholipids. Phylogenetic analysis of 16S rRNA gene sequences showed the strain formed distinct clade within the genus Streptomyces and was closely related to Streptomyces echinatus NBRC 12763 T (98.78 % 16S rRNA gene sequence similarity). According to the polyphasic approach as well as DNA-DNA relatedness, the strain could be clearly differentiated from closely related species and represents a novel species of the genus Streptomyces, for which the name Streptomyces actinomycinicus sp. nov. is proposed. The type strain is RCU-197 T (5JCM 30864 T 5TISTR 2208 T 5PCU 342 T ).
The novel actinomycete strain PWB-003 T , which produced fluostatins B and C antibiotics, was isolated from nearshore sediment collected from Panwa Cape, Phuket Province, Thailand. Data from the present polyphasic study indicated that strain PWB-003 T represented a member of the genus Micromonospora. It produced single spores on substrate mycelia and contained mesodiaminopimelic acid in the cell-wall peptidoglycan. Whole-cell hydrolysate contained ribose, xylose, arabinose, mannose and glucose. The predominant menaquinone was MK-10 (H 4 ). Cellular fatty acids comprised C 18 : 1 v9c, iso-C 16 : 0 , anteiso-C 17 : 0 , iso-C 15 : 0 and iso-C 17 : 0 . On the basis of 16S rRNA gene sequence similarity analysis, the novel strain was closely related to Micromonospora eburnea LK2-10 T (99.38 %), Micromonospora chaiyaphumensis
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