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Eleven strains of homofermentative, rod-shaped lactic acid bacteria and five strains of heterofermentative, sphere-shaped lactic acid bacteria were isolated from fermented fish (pla-ra and pla-chom) in Thailand. They were identified as new species and named Lactobacillus acidipiscis sp. nov. and Weissella thailandensis sp. nov., respectively, on the basis of phylogenetic analysis of the 16S rRNA gene sequences, DNA relatedness and phenotypic characteristics. The type strain of L. acidipiscis is FS60-1

A Gram-stain-negative, aerobic, non-spore-forming, rod-shaped bacterium, designed strain 201-F6 T , was isolated from a microbial consortium that degrades poly(ethylene terephthalate) (PET) collected in Sakai city, Japan, and was characterized on the basis of a polyphasic taxonomic study. The cells were motile with a polar flagellum. The strain contained cytochrome oxidase and catalase. It grew within the pH range 5.5-9.0 (optimally at pH 7-7.5) and at 15-42 ºC (optimally at 30-37 ºC). The major isoprenoid quinone was ubiquinone with eight isoprene units (Q-8). C 16 : 0 , C 17 : 0 cyclo, C 18 :1 !7c and C 12 : 0 2-OH were the predominant cellular fatty acids. The major polar lipids were phosphatidylethanolamine, lyso-phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The G+C content of genomic DNA was 70.4 mol%. Phylogenetic analysis using the 16S rRNA gene sequences showed that strain 201-F6 T was affiliated to the genus Ideonella, and was closely related to Ideonella dechloratans LMG 28178T (97.7 %) and Ideonella azotifigens JCM 15503 T (96.6 %). Strain 201-F6 T could be clearly distinguished from the related species of the genus Ideonella by its physiological and biochemical characteristics as well as by its phylogenetic position and DNA-DNA relatedness. Therefore, the strain represents a novel species of the genus Ideonella, for which the name Ideonella sakaiensis sp.

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Three bacterial strains were isolated from flowers collected in Bangkok, Thailand, by an enrichment-culture approach for acetic acid bacteria. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates were located in the lineage of the genus Asaia but constituted a cluster separate from the type strains of Asaia bogorensis and Asaia siamensis. The DNA base composition of the isolates was 60?2-60?5 mol% G+C, with a range of 0?3 mol%. The isolates constituted a taxon separate from Asaia bogorensis and Asaia siamensis on the basis of DNA-DNA relatedness. The isolates had morphological, physiological, biochemical and chemotaxonomic characteristics similar to those of the type strains of Asaia bogorensis and Asaia siamensis, but the isolates grew on maltose. The major ubiquinone was Q 10 . On the basis of the results obtained, the name Asaia krungthepensis sp. nov. is proposed for the isolates. The type strain is isolate AA08 T (=BCC 12978 T =TISTR 1524 T =NBRC 100057 T =NRIC 0535 T ), which had a DNA G+C content of 60?3 mol% and was isolated from a heliconia flower ('paksaasawan' in Thai; Heliconia sp.) collected in Bangkok, Thailand.The genus Asaia was introduced with a single species, Asaia bogorensis, as the fifth genus of the family Acetobacteraceae (Yamada et al., 2000). Asaia siamensis, the second species, was described by Katsura et al. (2001). Strains assigned to the genus Asaia are characterized by poor or non-existent production of acetic acid from ethanol and by the absence of growth in the presence of 0?35 % acetic acid (v/v). On the other hand, strains of the genera Acetobacter, Gluconobacter, Acidomonas, Gluconacetobacter and Kozakia produce large amounts of acetic acid from ethanol and grow in the presence of 0?35 % acetic acid. According to Yamada et al. (2000) and Katsura et al. (2001), strains of the genus Asaia have been isolated mostly from tropical flowers. During the course of our studies on acetic acid bacteria isolated from flowers collected in Thailand, we found that three isolates constitute a novel species; this paper describes Asaia krungthepensis sp. nov.Three bacterial strains were isolated from heliconia flowers ('paksaasawan' in Thai; Heliconia sp.) collected in Bangkok, Thailand, by an enrichment-culture approach using a sucrose medium, which was composed of 2?0 % sucrose, 0?3 % peptone, 0?3 % yeast extract and 0?3 % acetic acid (v/v) and adjusted to pH 3?5. When bacterial growth was found in the sucrose medium, micro-organisms were streaked on agar plates containing 2?0 % D-glucose, 0?5 % ethanol (v/v), 0?3 % peptone, 0?3 % yeast extract, 0?7 % CaCO 3 and 1?2 % agar (w/v). These acetic acid bacteria were selected as acid-producing bacterial strains that formed clear zones around colonies on agar plates. The three isolates, AA08 T (=BCC 12978 T ), AA09 (=BCC 12979) and AA06 (=BCC 12977), were cultured at 30 uC on agar slants comprising glucose/ethanol/CaCO 3 /agar medium (GECA medium) that contained 2?5 % D-glucose, 0?5 % ethanol, 3Present address: 2-3-21 Seinan...