Justicia hansenii is described and illustrated from south-eastern Thailand. Justicia henicophylla and J. viridiflora are lectotypified, and four names are placed into synonymy for the first time.
During the preparation of Boraginaceae and related families for the Flora of Thailand, a collection from limestone habitat in Uthai Thani province in March 2013 has been identified as Ehretia silvana R.R.Mill, which represents a new record for Thailand.
Endophytic actinobacterial strain 3R004T was isolated from a root of Justicia subcoriacea collected in Thailand. In this report, the taxonomic position of this strain is described using a polyphasic approach. Based on the morphological characteristics and chemical composition of its cells, strain 3R004T was identified as a member of the genus
Streptomyces
. It produced a long chain of cylindrical spores on aerial mycelia. ll-Diaminopimelic acid was detected in the cell wall peptidoglycan. The menaquinones were MK-9(H4), MK-9(H6) and MK-9(H8). C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and iso-C15 : 0 were detected as the major cellular fatty acids. Polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and one unidentified lipid. Strain 3R004T showed the highest 16S rRNA gene similarity of 99.45 % to
Streptomyces cyaneochromogenes
MK-45T. The phylogenomic results indicated that strain 3R004T was close to
Streptomyces aquilus
GGCR-6T and
Streptomyces antibioticus
DSM 40234T. The DNA–DNA hybridization and average nucleotide identity values among strain 3R004T and closely related
Streptomyces
species were 35.5–63.1 % and 82.7–94.3 %, respectively. The type strain produced actinomycin D antibiotic as the major secondary metabolite. The maximum productivity of the actinomycin D (378 mg l−1) was observed when the strain was grown in 301 broth at 30 °C, 180 r.p.m. for 12 days. On the basis of phenotypic and genotypic evidence, strain 3R004T represents a novel species of the genus
Streptomyces
, for which the name Streptomyces justiciae is proposed. The type strain is 3R004T (=LMG 32138T=TBRC 13128T=NBRC 115065T).
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