Pyrosequencing based profiling of the bacterial community in the Chilika Lake, the largest lagoon of India

Abstract

Brackish water lake is the most extraordinary reservoir for bacterial community with an adaptability of tolerance to saline stress. In the present study, metagenomic approach was implemented utilising 454-pyrosequencing platform to gain deeper insights into the bacterial diversity profile of the soil sediment of Chilika Lake, Odisha, India. Metagenome contained 68,150 sequences with 31,896,430 bp and 56.79% G + C content. Metagenome sequences data are now available at NCBI under the Sequence Read Archive (SRA) database with accession no. SRX753382. Bacterial community metagenome sequences were analysed by MG-RAST server representing the presence of 16,212 species belonging to 45 different phyla. The dominating phyla were Proteobacteria, Chloroflexi, Firmicutes, Acidobacteria, Actinobacteria, Bacteroidetes and Planctomycetes. The analysis of bacterial community datasets obtained from two different saline soil sediments revealed significant differences in bacterial community composition and diversity value providing better understanding of the ecosystem dynamics of Chilika Lake.

Metagenomic analysis exploring taxonomic and functional diversity of bacterial communities of a Himalayan urban fresh water lake

“…Freshwater lakes consist of 0.26% of total fresh water and 0.007% of total water on earth. The diversity of unculturable lake microbiota provides vast insights for microbiologists to investigate metagenome ecology for taxonomic identification and to study ecological implications [ 3 , 4 ].…”

Spatial analysis of bacteria in brackish lake sediment

“…Sea (Herlemann et al, 2011), in sediment of Sundarbans Mangrove Wetland (Basak et al, 2014), in two samples in Chilika Lake (Pramanik et al, 2015), and in a Brazilian mangrove (Andreote et al, 2012). Salinity was the only variable that was identified as having a spatial gradient of distribution at the scale of the current sampling in the lagoon.…”

Bacterial diversity of Murlen National Park located in Indo-Burman Biodiversity hotspot region: A metagenomic approach

“…Next-generation sequencing technology has been successfully applied to study bacterial diversity in different environments such as bat guano, hot spring, lake, cave sediments, etc. [5] , [6] , [7] , [8] .…”