Stereochemical studies on the microquantitative detection of vitamin D by gas-liquid chromatography

Nair, Padmanabhan P.; deLeon, Stella

doi:10.1016/0003-9861(68)90076-3

Cited by 23 publications

(3 citation statements)

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“…A 3% JXR column was used in the identification study. Stereochemical studies on the microquantitative detection have been made by Nair and deLeon (32), extending their previous study (23). It was confirmed that the 9,10-secosterols having a 5,6-cis configuration contribute to the structural features favoring cyclization at elevated GLC temperatures.…”

Section: Vl Tami N Dmentioning

confidence: 63%

“…The increased sensitivity reported for the heptafluorobutyrate esters allowed for the estimation of at least 10 ng of vitamins D z and D 3. It appears that the usable sensitivity of the methods of Nair and deLeon (32) and Wilson and coworkers (35) using the isotachysteryl heptafluorobutyrate esters are similar: between 1 and 10 ng with a 63Ni electron capture detector.…”

Section: 6-trans-vitamin D Undergoes Double-bond Rearrangementmentioning

confidence: 92%

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Gas chromatography of the fat‐soluble vitamins: A review

Sheppard

Prosser

Hubbard

1972

J Americ Oil Chem Soc

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The application of gas liquid chromatography (GLC) as an analytical tool for the determination of the fat-soluble vitamins A, D, E and K has yet to be utilized to its full potential. A review of the published work of many researchers in this field is presented. GLC methods to measure the vitamin A isomers have not been developed to any appreciable practical extent. Liquid liquid chromatography might well be the technique of choice. In the field of vitamin D there are indications that a practical GLC analysis is feasible for pharmaceutical preparations. The GLC applications for vitamin E are diverse, well defined and generally widely accepted in research laboratory situations and for regulatory and quality control usage. Vitamins K 1 and K 2 have been measured with limited success in a few research laboratories, but the GLC methods have not developed on a practical basis. However GLC is used for measuring vitamin K 3 (menadione and menadione sodium bisulfite) on a fairly routine basis in quality control laboratories.

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