Gas chromatography of the fat‐soluble vitamins: A review

Abstract

The application of gas liquid chromatography (GLC) as an analytical tool for the determination of the fat-soluble vitamins A, D, E and K has yet to be utilized to its full potential. A review of the published work of many researchers in this field is presented. GLC methods to measure the vitamin A isomers have not been developed to any appreciable practical extent. Liquid liquid chromatography might well be the technique of choice. In the field of vitamin D there are indications that a practical GLC analysis is feasible for pharmaceutical preparations. The GLC applications for vitamin E are diverse, well defined and generally widely accepted in research laboratory situations and for regulatory and quality control usage. Vitamins K 1 and K 2 have been measured with limited success in a few research laboratories, but the GLC methods have not developed on a practical basis. However GLC is used for measuring vitamin K 3 (menadione and menadione sodium bisulfite) on a fairly routine basis in quality control laboratories.

Column fractionation of polymers. XXXI. Gel permeation chromatography. Mechanism of separation by flow

“…Horta (3) has suggested that polymer flow separation by molecular weight should be more effective in good polymer solvents. Yau, Malone, and Suchan (4) show peak separation in conventional GPC is mainly dominated by the pro-13) A. Horta, J. Macromol.…”

Analysis of vitamin K1 in some green leafy vegetables by gas chromatography

“…Schilling and Dam's (1958) colorimetric method was applied only to alfalfa, and interfering substances could affect the accuracy of the method. Aaron and Winefordner (1972), in their publication of a phosphorimetry method for pure K1( reviewed the literature and found few quantitative determinations of naturally occurring Kv The comprehensive review of gas chromatography (GLC) of the fat-soluble vitamins by Sheppard et al (1972) discussed the results of various GLC assays for Kj and analogues.…”

“…Initially, Nair and Turner (1963), working with lipid extracts and synthetic mixtures, and later Carroll and Herting (1964) (1967), using synthetic mixtures, showed the feasibility of separating Kx by GLC. A number Western Regional Research Center, Science and Education Administration, U.S. Department of Agriculture, Berkeley, California 94710. of other workers, reviewed by Sheppard et al (1972) and cited by Aaron and Winefordner (1972), explored the possibilities of GLC assay. Sheppard and Hubbard (1971) described a GLC method for K4 in liver tissue but did not report any results.…”

Competitive Protein-Binding Assay for 25-Hydroxycholecalciferol

“…The assay of cholecalciferol levels in animal tissues by physicochemical methods has not hitherto been possible, due in part to the problems of purification and in part to the need for a method with high sensitivity (for review see Kodicek & Lawson, 1967;Sheppard, Proser & Hubbard, 1972). The antimony trichloride colorimetric reaction, which is widely used for the estimation of cholecalciferol, particularly in high-potency preparations such as fish oils, lacks sensitivity and specificity.…”