Abstract:Background Implant placement in defective anterior maxilla poses a great challenge regarding functional and aesthetic outcomes. Therefore, it requires predictable alveolar ridge augmentation. Deproteinized bovine bone mineral (DBBM) particle has commonly been used for bone grafting. However, it is associated with low resorption rates which potentially compromise the outcome of horizontal augmentation in conjunction with implant placement. Aims This study is aimed at evaluating the stability of tissue augmented… Show more
“…In a previous study, DBBMs showed a slow degradation rate and relatively low bone regeneration capacity [ 26 ]. Non-mineralized bone substitutes promote osteoblast differentiation by releasing bone morphogenic proteins.…”
Purpose
The aim of this study was to evaluate the preclinical results of 2 types of commercially available deproteinized bovine bone mineral (DBBM) when applied to alveolar bone defects in dogs.
Methods
This study was conducted using 6 beagles. Alveolar defects in the mandible were formed and filled with 2 DBBMs produced by a similar procedure. Defects were randomly assigned to be filled using DBBM 1 or 2. All defects were covered with a collagen membrane and had a healing period of 12 weeks. After the dogs were sacrificed, histological, histomorphometric, and linear/volumetric analyses were performed.
Results
Both DBBM groups showed similar histological findings, demonstrating that bone remodeling had occurred and new bone had formed. The residual bone particles were surrounded by newly formed vital bone. In the histomorphometric analysis, the ratio of the area of vital bone and residual bone substitute in DBBM 2 (38.18% and 3.47%, respectively) was higher than that of DBBM 1 (33.74% and 3.41%, respectively), although the difference was not statistically significant. There were also no statistically significant differences between both groups in linear and volumetric analyses using micro-computed tomography scans and digitized images of dental casts.
Conclusions
In the present study, DBBM 1and 2, which were produced by similar processes, showed similar results in histological, histomorphometric, and volumetric analyses. Further studies are needed to identify more specific differences between the 2 DBBMs.
“…In a previous study, DBBMs showed a slow degradation rate and relatively low bone regeneration capacity [ 26 ]. Non-mineralized bone substitutes promote osteoblast differentiation by releasing bone morphogenic proteins.…”
Purpose
The aim of this study was to evaluate the preclinical results of 2 types of commercially available deproteinized bovine bone mineral (DBBM) when applied to alveolar bone defects in dogs.
Methods
This study was conducted using 6 beagles. Alveolar defects in the mandible were formed and filled with 2 DBBMs produced by a similar procedure. Defects were randomly assigned to be filled using DBBM 1 or 2. All defects were covered with a collagen membrane and had a healing period of 12 weeks. After the dogs were sacrificed, histological, histomorphometric, and linear/volumetric analyses were performed.
Results
Both DBBM groups showed similar histological findings, demonstrating that bone remodeling had occurred and new bone had formed. The residual bone particles were surrounded by newly formed vital bone. In the histomorphometric analysis, the ratio of the area of vital bone and residual bone substitute in DBBM 2 (38.18% and 3.47%, respectively) was higher than that of DBBM 1 (33.74% and 3.41%, respectively), although the difference was not statistically significant. There were also no statistically significant differences between both groups in linear and volumetric analyses using micro-computed tomography scans and digitized images of dental casts.
Conclusions
In the present study, DBBM 1and 2, which were produced by similar processes, showed similar results in histological, histomorphometric, and volumetric analyses. Further studies are needed to identify more specific differences between the 2 DBBMs.
“…Osteoclasts could be associated with the resorption of the e quantification of protein expressions showed that osteoblastic markers were significantly higher in the DFDBBX group compared to the DBBM group (p < 0.05), except in ALP in 4 weeks (n � 5); * p < 0.05. 6 International Journal of Dentistry surrounding and the woven bone in the DFDBBX group during the late stage of healing. It is noteworthy, however, that the highest number of osteoclasts was seen in the control group indicating a consistent increase in resorption activities in 2 to 4 weeks.…”
Section: Discussionmentioning
confidence: 99%
“…eir high osteoconductive activity allows cell migration, attachment, and proliferation, osteoblastic differentiation, and extracellular bone matrix deposition [5]. However, DBBM particles have a slow degradation rate and, consequently, relatively low bone regeneration capacity [6]. Demineralized freeze-dried bovine bone xenograft (DFDBBX) has been developed to overcome DBBM limitations [7].…”
Background. Deproteinized bovine bone mineral (DBBM) particle is the commonly used bone graft substitute in implant surgery which is mainly osteoconductive and has very slow degradation. Demineralized freeze-dried bovine bone xenograft (DFDBBX) particle is being developed as a novel xenogeneic bone filler. Objectives. The study aimed to analyze osteogenic activity and bone-forming capacity of DFDBBX particles compared to DBBM particles in alveolar bone defects in rabbit mandibles models. Material and Methods. This study investigated bone defects whether filled with DBBM particles or DFDBBX particles or left unfilled in 30 rabbit mandibles. Specimens were processed for histology, immunohistochemistry, and micro-CT scanning. Statistical difference was set at a
p
value < 0.05. Results. The quantitative assessment showed a significantly lower number of osteoclasts and a higher number of osteoblasts in the DFDBBX group compared to the DBBM group in 2 and 4 weeks (
p
<
0.05
). Immunostaining analyses showed significantly higher expression of RUNX2, collagen type I, alkaline phosphatase, and osteocalcin in the DFDBBX group compared to the DBBM group in 2 and 4 weeks. Bone healing score in the DFDBBX group was comparable to the DBBM group. Micro-CT presented no significant difference in the volume percentage of the mineralized tissue in the DBBM and DFDBBX groups in spite of the different healing patterns in both groups. Conclusion. DFDBBX particles induced higher osteoblastic activities than DBBM particles at the early stage of healing. Meanwhile, the capacity of bone formation in DFDBBX particles was comparable with DBBM particles at the later stage of healing. Considering the limitation of this study, the results presented DFDBBX particles as potential bone filler candidates.
“… 14 However, it was associated with very slow degradation that might lower its bone regeneration capacity. 4 In order to overcome DBBM limitation, mineralized FDBX and DFDBXs were developed recapitulating the concept and processing of human FDBA and DFDBA. 15 Despite the concern over the potential xenogeneic response upon its clinical application, this organic forms of xenograft material did not show abnormal immune response in few in vitro 7 16 17 18 and in vivo studies 7 9 19 as well as in a clinical study.…”
Section: Discussionmentioning
confidence: 99%
“…The DBBM particle is purely inorganic that is considered osteoconductive and exhibits extremely slow degradation which may reduce the bone regeneration capacity and bone-implant contact area. 4 5 Limitation of DBBM particle has made way to the utilization of other forms of bovine bone particles, that is, freeze-dried bovine bone particles (FDBX) and demineralized freeze-dried bovine bone particle (DFDBX) recapitulating the processing in allograft production. 6 Despite concerns over the potential xenogeneic response upon its application, these organic forms of xenograft material is proven to be nontoxic and nonimmunogenic in in vitro and in vivo studies.…”
Objective This study aimed to evaluate bone regeneration capacity of FDBX granules compared to composite DBBM/DFDBX granules for filling of bone defect in rabbit mandible.
Material and Methods Critical size defects were created in 45 rabbits' mandible. The defect in the control group is left untreated, while in other groups the defects were filled with FDBX granules and composite DBBM/DFDBX granules, respectively. Specimens were collected at 2, 4, and 8 weeks for histology and immunohistochemical analyses. Significant difference is set at p-value < 0.05.
Results The osteoblast-osteoclast quantification, osteoblast expression of Runx2, alkaline phosphatase, collagen-I, and osteocalcin, and osteoclast expression of receptor activator of NF-kB ligand (RANKL) and osteoprotegerin (OPG) in FDBX groups were statistically comparable (p > 0.05) with the composite group, while OPG/RANKL ratio, bone healing scores, and trabecular area were significantly higher (p < 0.05) in the composite compared to FDBX group.
Conclusion Composite DBBM/DFDBX granules, within the limitation of this study, has better bone forming capacity than FDBX granules for filling of bone defects in the mandible.
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