Background. Deproteinized bovine bone mineral (DBBM) particle is the commonly used bone graft substitute in implant surgery which is mainly osteoconductive and has very slow degradation. Demineralized freeze-dried bovine bone xenograft (DFDBBX) particle is being developed as a novel xenogeneic bone filler. Objectives. The study aimed to analyze osteogenic activity and bone-forming capacity of DFDBBX particles compared to DBBM particles in alveolar bone defects in rabbit mandibles models. Material and Methods. This study investigated bone defects whether filled with DBBM particles or DFDBBX particles or left unfilled in 30 rabbit mandibles. Specimens were processed for histology, immunohistochemistry, and micro-CT scanning. Statistical difference was set at a p value < 0.05. Results. The quantitative assessment showed a significantly lower number of osteoclasts and a higher number of osteoblasts in the DFDBBX group compared to the DBBM group in 2 and 4 weeks ( p < 0.05 ). Immunostaining analyses showed significantly higher expression of RUNX2, collagen type I, alkaline phosphatase, and osteocalcin in the DFDBBX group compared to the DBBM group in 2 and 4 weeks. Bone healing score in the DFDBBX group was comparable to the DBBM group. Micro-CT presented no significant difference in the volume percentage of the mineralized tissue in the DBBM and DFDBBX groups in spite of the different healing patterns in both groups. Conclusion. DFDBBX particles induced higher osteoblastic activities than DBBM particles at the early stage of healing. Meanwhile, the capacity of bone formation in DFDBBX particles was comparable with DBBM particles at the later stage of healing. Considering the limitation of this study, the results presented DFDBBX particles as potential bone filler candidates.

Autogenous bone graft is the gold standard for bone defects treatment, however due to their limitation and the donor site morbidity may caused many surgeons use a xenograft type of bone grafting to cope the problem. Demineralized Freeze Dried Bone Xenograft (DFDBBX) which contains of growth factors, have a good biocompatibility. The aim of this study is observed the difference in bone healing processes between DFDBBX and Bovine Bone Hydroxyapatite Xenograft (BBHAX). Bicortical bone defects were created in the mandibular corpus of 30 New Zealand White Rabbits. The groups were divided into 3 groups which the first group were treated with DFDBBX into the hole and the negative control group was left perforated. The other group was treated with BBHAX. All group were evaluated after second and fourth weeks to count the ammount of osteoblast, osteoclast cells, Collagen-1 (Coll-1) and alkalin phosphatase (ALP). The second week of observation showed a significant difference of mean 12,45, SD 2,97 (p<0,05) in osteoblast cells. In Coll-1 showed with mean 13,2 SD 2,68 (p<0,05). The result of ALP showed with mean 14,6 SD 2,70 (p<0,05). In the the fourth week observation showed increased of osteoclast cells with mean 7,043, SD 2,77 (p< 0,05) and for Coll-1 with mean 17,6, SD 2,30 (p< 0,05). DFDBBX showed more effective in treating bone defects of mandible of new zealand white rabbits in second week of observation.

Objective:Present serial case management of temporomandibular joint dislocation in emergency unit and to provide information on emergency handling in the case of temporomandibula joint especially for dentist.Method: Datas of temporomandibular joint dislocation cases were collected from October 2016 to March 2017 with a cross sectional study presented by Oral and Maxillofacial Resident in RSUD Dr Soetomo Surabaya.Results: Nine patients were managed. In totalmales accounted for 66,67% of the patients, and yawning was the most frequent etiological factor. Acute TMJ dislocation had the highest frequency in this study. The management conducted was giving muscle relaxant, manual repositioning and head bandage. Manual reduction of TMJ dislocation can be divided into three approaches, intraorally, extraorally and a combination between the two approaches; intraorally by using Hippocrates’ technique and Wrist Pivot technique. The extraoral approach does not involve fingers in the oral cavity. Applying these three approaches could be distinguished by clinical examination and patient history.Conclusion: Temporomandibular joint dislocation is one of the problem in dentistry that requires direct management and could cause complications if immediate treatment is not givenKeywords: Dislocations, Manual Reduction , Temporomandibular joint